Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.

We developed a label-free method for a determination of the number of biomolecules attached to individual cells by measuring the electrophoretic mobility of the cells in a microchannel. The surface of a biological cell, which is dispersed in aqueous solution, is normally electrically charged and the...

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Autores principales:	Atsushi Aki, Baiju G Nair, Hisao Morimoto, D Sakthi Kumar, Toru Maekawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56911b36caf34976ac27c3dba709810f
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spelling	oai:doaj.org-article:56911b36caf34976ac27c3dba709810f2021-11-18T07:01:06ZLabel-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.1932-620310.1371/journal.pone.0015641https://doaj.org/article/56911b36caf34976ac27c3dba709810f2010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21206908/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We developed a label-free method for a determination of the number of biomolecules attached to individual cells by measuring the electrophoretic mobility of the cells in a microchannel. The surface of a biological cell, which is dispersed in aqueous solution, is normally electrically charged and the charge quantity at the cell's surface is slightly changed once antibody molecules are attached to the cell, based on which we detect the attachment of antibody molecules to the surface of individual red blood cells by electrophoretic mobility measurement. We also analyzed the number of antibody molecules attached to the cell's surface using a flow cytometer. We found that there is a clear correlation between the number of antibody molecules attached to the individual cells and the electrophoretic mobility of the cells. The present technique may well be utilized not only in the field of cell biology but also in the medical and pharmaceutical industries.Atsushi AkiBaiju G NairHisao MorimotoD Sakthi KumarToru MaekawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15641 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsushi Aki Baiju G Nair Hisao Morimoto D Sakthi Kumar Toru Maekawa Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
description	We developed a label-free method for a determination of the number of biomolecules attached to individual cells by measuring the electrophoretic mobility of the cells in a microchannel. The surface of a biological cell, which is dispersed in aqueous solution, is normally electrically charged and the charge quantity at the cell's surface is slightly changed once antibody molecules are attached to the cell, based on which we detect the attachment of antibody molecules to the surface of individual red blood cells by electrophoretic mobility measurement. We also analyzed the number of antibody molecules attached to the cell's surface using a flow cytometer. We found that there is a clear correlation between the number of antibody molecules attached to the individual cells and the electrophoretic mobility of the cells. The present technique may well be utilized not only in the field of cell biology but also in the medical and pharmaceutical industries.
format	article
author	Atsushi Aki Baiju G Nair Hisao Morimoto D Sakthi Kumar Toru Maekawa
author_facet	Atsushi Aki Baiju G Nair Hisao Morimoto D Sakthi Kumar Toru Maekawa
author_sort	Atsushi Aki
title	Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
title_short	Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
title_full	Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
title_fullStr	Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
title_full_unstemmed	Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
title_sort	label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/56911b36caf34976ac27c3dba709810f
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Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.

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