Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS

Abstract The decrease of gelsolin (GSN) in the blood has been reported in multiple sclerosis (MS) patients and experimental allergic encephalomyelitis (EAE) animals, but the protective effect of GSN on EAE/MS lacks of evidence. In our study, we increased the GSN level in EAE by injecting GSN-overexp...

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Autores principales:	Jifang Gao, Zhaoyu Qin, Xinyuan Guan, Juanjuan Guo, Huaqing Wang, Shilian Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56916c6e9c2443ec8fa7ad319e515f72
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spelling	oai:doaj.org-article:56916c6e9c2443ec8fa7ad319e515f722021-12-02T11:53:03ZOverexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS10.1038/s41598-017-00684-w2045-2322https://doaj.org/article/56916c6e9c2443ec8fa7ad319e515f722017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00684-whttps://doaj.org/toc/2045-2322Abstract The decrease of gelsolin (GSN) in the blood has been reported in multiple sclerosis (MS) patients and experimental allergic encephalomyelitis (EAE) animals, but the protective effect of GSN on EAE/MS lacks of evidence. In our study, we increased the GSN level in EAE by injecting GSN-overexpress lentivirus (LV-GSN) into the lateral ventricle and caudal vein and found that GSN administration can delay the onset and decrease the severity of EAE. Vitamin D is proven to have a therapeutic effect on MS/EAE; however, we previously found that vitamin D caused a downregulation of GSN, which might limit vitamin D efficacy. In our current research, we obtained a better symptom and a slowing down progression in EAE after combining vitamin D treatment with a proper increase of GSN. Furthermore, we discovered that the mediation of vitamin D on GSN might occur through the vitamin D receptor (VDR) by using gene interruption and overexpression to regulate the level of VDR in PC12 cells (a rat sympathetic nerve cell line). We also confirmed the anti-apoptotic function of GSN by GSN RNA interference in PC12. Collectively, these results support the therapeutic effect of GSN in EAE, which might enhance Vitamin D therapy in EAE/MS.Jifang GaoZhaoyu QinXinyuan GuanJuanjuan GuoHuaqing WangShilian LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jifang Gao Zhaoyu Qin Xinyuan Guan Juanjuan Guo Huaqing Wang Shilian Liu Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
description	Abstract The decrease of gelsolin (GSN) in the blood has been reported in multiple sclerosis (MS) patients and experimental allergic encephalomyelitis (EAE) animals, but the protective effect of GSN on EAE/MS lacks of evidence. In our study, we increased the GSN level in EAE by injecting GSN-overexpress lentivirus (LV-GSN) into the lateral ventricle and caudal vein and found that GSN administration can delay the onset and decrease the severity of EAE. Vitamin D is proven to have a therapeutic effect on MS/EAE; however, we previously found that vitamin D caused a downregulation of GSN, which might limit vitamin D efficacy. In our current research, we obtained a better symptom and a slowing down progression in EAE after combining vitamin D treatment with a proper increase of GSN. Furthermore, we discovered that the mediation of vitamin D on GSN might occur through the vitamin D receptor (VDR) by using gene interruption and overexpression to regulate the level of VDR in PC12 cells (a rat sympathetic nerve cell line). We also confirmed the anti-apoptotic function of GSN by GSN RNA interference in PC12. Collectively, these results support the therapeutic effect of GSN in EAE, which might enhance Vitamin D therapy in EAE/MS.
format	article
author	Jifang Gao Zhaoyu Qin Xinyuan Guan Juanjuan Guo Huaqing Wang Shilian Liu
author_facet	Jifang Gao Zhaoyu Qin Xinyuan Guan Juanjuan Guo Huaqing Wang Shilian Liu
author_sort	Jifang Gao
title	Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
title_short	Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
title_full	Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
title_fullStr	Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
title_full_unstemmed	Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS
title_sort	overexpression of gsn could decrease inflammation and apoptosis in eae and may enhance vitamin d therapy on eae/ms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/56916c6e9c2443ec8fa7ad319e515f72
work_keys_str_mv	AT jifanggao overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems AT zhaoyuqin overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems AT xinyuanguan overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems AT juanjuanguo overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems AT huaqingwang overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems AT shilianliu overexpressionofgsncoulddecreaseinflammationandapoptosisineaeandmayenhancevitamindtherapyoneaems
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Overexpression of GSN could decrease inflammation and apoptosis in EAE and may enhance vitamin D therapy on EAE/MS

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