Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)

Abstract. Nelly N, Khairul U, Putri AY, Hamid H, Syahrawati MY. 2020. Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura F (Lepidoptera: Noctuidae). Biodiversitas 21: 753-758. Spodoptera litura is a polyphagous pest that can be cont...

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Autores principales:	Novri Nelly, Ujang Khairul, Ade Yulia Lestari, Hasmiandy Hamid, My Syahrawati
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	control, corn rhizosphere, entomopathogen, rhizobacteria, spodoptera litura Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5691dbc14cf44e139474665e0814c325
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spelling	oai:doaj.org-article:5691dbc14cf44e139474665e0814c3252021-11-21T22:01:10ZIsolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)1412-033X2085-472210.13057/biodiv/d210243https://doaj.org/article/5691dbc14cf44e139474665e0814c3252020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4534https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nelly N, Khairul U, Putri AY, Hamid H, Syahrawati MY. 2020. Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura F (Lepidoptera: Noctuidae). Biodiversitas 21: 753-758. Spodoptera litura is a polyphagous pest that can be controlled with entomopathogen. Furthermore, Rhizobacteria derived from the rhizosphere of corn plants are expected to control this pest. This research, therefore, was conducted to obtain the entomopathogenic rhizobacteria to control S. litura derived from the rhizosphere of corn plants. Data sampling was carried out at corn plantations in West Pasaman Regency, while Isolation, selection, and testing of S. litura were conducted at the Biological Control Laboratory of the Plant Protection Department, Faculty of Agriculture, Andalas University. The method uses serial glowing techniques cultured on Nutrient Agar (NA) media, with the selected isolates tested for their ability as an entomopathogen against S. litura. The feed dip method given to S. litura larvae was used to test for rhizobacteria virulence determination with entomopathogen, used to observe the larvae mortality rate, percentage of pupae, and adult formed. Furthermore, observation of morphological and physiological characters was conducted on entomopathogenic rhizobacteria, with hypersensitive testing conducted on Mirabilis jalapa plants. The results of rhizobacterial isolation from corn rhizosphere obtained 12 entomopathogenic isolates. Deadly larvae of S. litura 51-91.11%, 0.00-8.89% pupae, and 0.00- 6.67% adult were formed. The gram test results obtained approximately 8;4 positive (+) and four negative isolates (-), respectively. Hypersensitivity test results found that one of 12 isolates caused necrotic reactions in Mirabilis jalapa. Therefore, a total of 11 rhizobacteria isolates are entomopathogenic and safe to be used as S. litura biological control agents.Novri NellyUjang KhairulAde Yulia LestariHasmiandy HamidMy SyahrawatiMBI & UNS Soloarticlecontrol, corn rhizosphere, entomopathogen, rhizobacteria, spodoptera lituraBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	control, corn rhizosphere, entomopathogen, rhizobacteria, spodoptera litura Biology (General) QH301-705.5
spellingShingle	control, corn rhizosphere, entomopathogen, rhizobacteria, spodoptera litura Biology (General) QH301-705.5 Novri Nelly Ujang Khairul Ade Yulia Lestari Hasmiandy Hamid My Syahrawati Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
description	Abstract. Nelly N, Khairul U, Putri AY, Hamid H, Syahrawati MY. 2020. Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura F (Lepidoptera: Noctuidae). Biodiversitas 21: 753-758. Spodoptera litura is a polyphagous pest that can be controlled with entomopathogen. Furthermore, Rhizobacteria derived from the rhizosphere of corn plants are expected to control this pest. This research, therefore, was conducted to obtain the entomopathogenic rhizobacteria to control S. litura derived from the rhizosphere of corn plants. Data sampling was carried out at corn plantations in West Pasaman Regency, while Isolation, selection, and testing of S. litura were conducted at the Biological Control Laboratory of the Plant Protection Department, Faculty of Agriculture, Andalas University. The method uses serial glowing techniques cultured on Nutrient Agar (NA) media, with the selected isolates tested for their ability as an entomopathogen against S. litura. The feed dip method given to S. litura larvae was used to test for rhizobacteria virulence determination with entomopathogen, used to observe the larvae mortality rate, percentage of pupae, and adult formed. Furthermore, observation of morphological and physiological characters was conducted on entomopathogenic rhizobacteria, with hypersensitive testing conducted on Mirabilis jalapa plants. The results of rhizobacterial isolation from corn rhizosphere obtained 12 entomopathogenic isolates. Deadly larvae of S. litura 51-91.11%, 0.00-8.89% pupae, and 0.00- 6.67% adult were formed. The gram test results obtained approximately 8;4 positive (+) and four negative isolates (-), respectively. Hypersensitivity test results found that one of 12 isolates caused necrotic reactions in Mirabilis jalapa. Therefore, a total of 11 rhizobacteria isolates are entomopathogenic and safe to be used as S. litura biological control agents.
format	article
author	Novri Nelly Ujang Khairul Ade Yulia Lestari Hasmiandy Hamid My Syahrawati
author_facet	Novri Nelly Ujang Khairul Ade Yulia Lestari Hasmiandy Hamid My Syahrawati
author_sort	Novri Nelly
title	Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
title_short	Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
title_full	Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
title_fullStr	Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
title_full_unstemmed	Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
title_sort	isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against spodoptera litura (lepidoptera: noctuidae)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/5691dbc14cf44e139474665e0814c325
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Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)

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