Regulation of T cell development and activation by creatine kinase B.

Creatine kinase catalyzes the reversible transfer of the N-phosphoryl group from phosphocreatine to ADP to generate ATP and plays a key role in highly energy-demanding processes such as muscle contraction and flagellar motility; however, its role in signal transduction (which frequently involves ATP...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yafeng Zhang, Hai Li, Xiaoming Wang, Xiang Gao, Xiaolong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5697f63c6d504ea391cc16c1523cc85e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5697f63c6d504ea391cc16c1523cc85e
record_format	dspace
spelling	oai:doaj.org-article:5697f63c6d504ea391cc16c1523cc85e2021-11-25T06:16:24ZRegulation of T cell development and activation by creatine kinase B.1932-620310.1371/journal.pone.0005000https://doaj.org/article/5697f63c6d504ea391cc16c1523cc85e2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19337362/?tool=EBIhttps://doaj.org/toc/1932-6203Creatine kinase catalyzes the reversible transfer of the N-phosphoryl group from phosphocreatine to ADP to generate ATP and plays a key role in highly energy-demanding processes such as muscle contraction and flagellar motility; however, its role in signal transduction (which frequently involves ATP-consuming phosphorylation) and consequent cell-fate decisions remains largely unknown. Here we report that creatine kinase B was significantly up-regulated during the differentiation of double-positive thymocytes into single-positive thymocytes. Ectopic expression of creatine kinase B led to increased ATP level and enhanced phosphorylation of the TCR signaling proteins. Consequentially, transgenic expression of creatine kinase B promoted the expression of Nur77 and Bim proteins and the cell death of TCR signaled thymocyte. In addition, the activation, proliferation and cytokine secretion of T cells were also enhanced by the expression of creatine kinase B transgene. In contrast, treatment of T cells with specific creatine kinase inhibitor or creatine kinase B shRNA resulted in severely impaired T cell activation. Taken together, our results indicate that creatine kinase B plays an unexpected role in modulating TCR-mediated signaling and critically regulates thymocyte selection and T cell activation.Yafeng ZhangHai LiXiaoming WangXiang GaoXiaolong LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5000 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yafeng Zhang Hai Li Xiaoming Wang Xiang Gao Xiaolong Liu Regulation of T cell development and activation by creatine kinase B.
description	Creatine kinase catalyzes the reversible transfer of the N-phosphoryl group from phosphocreatine to ADP to generate ATP and plays a key role in highly energy-demanding processes such as muscle contraction and flagellar motility; however, its role in signal transduction (which frequently involves ATP-consuming phosphorylation) and consequent cell-fate decisions remains largely unknown. Here we report that creatine kinase B was significantly up-regulated during the differentiation of double-positive thymocytes into single-positive thymocytes. Ectopic expression of creatine kinase B led to increased ATP level and enhanced phosphorylation of the TCR signaling proteins. Consequentially, transgenic expression of creatine kinase B promoted the expression of Nur77 and Bim proteins and the cell death of TCR signaled thymocyte. In addition, the activation, proliferation and cytokine secretion of T cells were also enhanced by the expression of creatine kinase B transgene. In contrast, treatment of T cells with specific creatine kinase inhibitor or creatine kinase B shRNA resulted in severely impaired T cell activation. Taken together, our results indicate that creatine kinase B plays an unexpected role in modulating TCR-mediated signaling and critically regulates thymocyte selection and T cell activation.
format	article
author	Yafeng Zhang Hai Li Xiaoming Wang Xiang Gao Xiaolong Liu
author_facet	Yafeng Zhang Hai Li Xiaoming Wang Xiang Gao Xiaolong Liu
author_sort	Yafeng Zhang
title	Regulation of T cell development and activation by creatine kinase B.
title_short	Regulation of T cell development and activation by creatine kinase B.
title_full	Regulation of T cell development and activation by creatine kinase B.
title_fullStr	Regulation of T cell development and activation by creatine kinase B.
title_full_unstemmed	Regulation of T cell development and activation by creatine kinase B.
title_sort	regulation of t cell development and activation by creatine kinase b.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/5697f63c6d504ea391cc16c1523cc85e
work_keys_str_mv	AT yafengzhang regulationoftcelldevelopmentandactivationbycreatinekinaseb AT haili regulationoftcelldevelopmentandactivationbycreatinekinaseb AT xiaomingwang regulationoftcelldevelopmentandactivationbycreatinekinaseb AT xianggao regulationoftcelldevelopmentandactivationbycreatinekinaseb AT xiaolongliu regulationoftcelldevelopmentandactivationbycreatinekinaseb
_version_	1718413982982733824

Regulation of T cell development and activation by creatine kinase B.

Ejemplares similares