Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density

X-ray micro-computed tomography (μ-CT) can be used to provide both qualitative and quantitative information on the structure of three-dimensional (3D) bioactive scaffolds. When performed in a dry state, μ-CT accurately reflects the structure of collagen-based scaffolds, but imaging in a wet state of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kyung-Ah Kwon, Daniel V. Bax, Jennifer H. Shepherd, Ruth E. Cameron, Serena M. Best
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/56a137cec150474b81baacf6e7145fea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:56a137cec150474b81baacf6e7145fea
record_format	dspace
spelling	oai:doaj.org-article:56a137cec150474b81baacf6e7145fea2021-11-04T04:35:15ZAvoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density2452-199X10.1016/j.bioactmat.2021.06.012https://doaj.org/article/56a137cec150474b81baacf6e7145fea2022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X2100298Xhttps://doaj.org/toc/2452-199XX-ray micro-computed tomography (μ-CT) can be used to provide both qualitative and quantitative information on the structure of three-dimensional (3D) bioactive scaffolds. When performed in a dry state, μ-CT accurately reflects the structure of collagen-based scaffolds, but imaging in a wet state offers challenges with radiolucency. Here we have used phosphotungstic acid (PTA) as a contrast agent to visualise fully hydrated collagen scaffolds in a physiologically relevant environment. A systematic investigation was performed to understand the effects of PTA on the results of μ-CT imaging by varying sample processing variables such as crosslinking density, hydration medium and staining duration.Immersing samples in 0.3% PTA solution overnight completely stained the samples and the treatment provided a successful route for μ-CT analysis of crosslinked samples. However, significant structural artefacts were observed for samples which were either non-crosslinked or had low levels of crosslinking, which had a heterogeneous interior architecture with collapsed pores at the scaffold periphery. This work highlights the importance of optimising the choice of processing and staining conditions to ensure accurate visualisation for hydrated 3D collagen scaffolds in an aqueous medium.Kyung-Ah KwonDaniel V. BaxJennifer H. ShepherdRuth E. CameronSerena M. BestKeAi Communications Co., Ltd.articleMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 210-219 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Kyung-Ah Kwon Daniel V. Bax Jennifer H. Shepherd Ruth E. Cameron Serena M. Best Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
description	X-ray micro-computed tomography (μ-CT) can be used to provide both qualitative and quantitative information on the structure of three-dimensional (3D) bioactive scaffolds. When performed in a dry state, μ-CT accurately reflects the structure of collagen-based scaffolds, but imaging in a wet state offers challenges with radiolucency. Here we have used phosphotungstic acid (PTA) as a contrast agent to visualise fully hydrated collagen scaffolds in a physiologically relevant environment. A systematic investigation was performed to understand the effects of PTA on the results of μ-CT imaging by varying sample processing variables such as crosslinking density, hydration medium and staining duration.Immersing samples in 0.3% PTA solution overnight completely stained the samples and the treatment provided a successful route for μ-CT analysis of crosslinked samples. However, significant structural artefacts were observed for samples which were either non-crosslinked or had low levels of crosslinking, which had a heterogeneous interior architecture with collapsed pores at the scaffold periphery. This work highlights the importance of optimising the choice of processing and staining conditions to ensure accurate visualisation for hydrated 3D collagen scaffolds in an aqueous medium.
format	article
author	Kyung-Ah Kwon Daniel V. Bax Jennifer H. Shepherd Ruth E. Cameron Serena M. Best
author_facet	Kyung-Ah Kwon Daniel V. Bax Jennifer H. Shepherd Ruth E. Cameron Serena M. Best
author_sort	Kyung-Ah Kwon
title	Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
title_short	Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
title_full	Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
title_fullStr	Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
title_full_unstemmed	Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density
title_sort	avoiding artefacts in microct imaging of collagen scaffolds: effect of phosphotungstic acid (pta)-staining and crosslink density
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/56a137cec150474b81baacf6e7145fea
work_keys_str_mv	AT kyungahkwon avoidingartefactsinmicroctimagingofcollagenscaffoldseffectofphosphotungsticacidptastainingandcrosslinkdensity AT danielvbax avoidingartefactsinmicroctimagingofcollagenscaffoldseffectofphosphotungsticacidptastainingandcrosslinkdensity AT jenniferhshepherd avoidingartefactsinmicroctimagingofcollagenscaffoldseffectofphosphotungsticacidptastainingandcrosslinkdensity AT ruthecameron avoidingartefactsinmicroctimagingofcollagenscaffoldseffectofphosphotungsticacidptastainingandcrosslinkdensity AT serenambest avoidingartefactsinmicroctimagingofcollagenscaffoldseffectofphosphotungsticacidptastainingandcrosslinkdensity
_version_	1718445273131253760

Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density

Ejemplares similares