A New PNA-FISH Probe Targeting Fannyhessea vaginae

Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth...

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Autores principales:	Lúcia G. V. Sousa, Joana Castro, Angela França, Carina Almeida, Christina A. Muzny, Nuno Cerca
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Fannyhessea vaginae Gardnerella vaginalis bacterial vaginosis fluorescence in situ hybridization (FISH) peptide nucleic acid (PNA) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/56ac04350bad4a72b65af79ab6352dac
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id	oai:doaj.org-article:56ac04350bad4a72b65af79ab6352dac
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spelling	oai:doaj.org-article:56ac04350bad4a72b65af79ab6352dac2021-11-18T09:35:59ZA New PNA-FISH Probe Targeting Fannyhessea vaginae2235-298810.3389/fcimb.2021.779376https://doaj.org/article/56ac04350bad4a72b65af79ab6352dac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.779376/fullhttps://doaj.org/toc/2235-2988Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood. Gardnerella spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless, Fannyhessea vaginae (previously known as Atopobium vaginae) is a highly specific species for BV, and co-colonization with Gardnerella is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting F. vaginae. This probe was further validated in a multiplex assay, which included a Gardnerella-specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate F. vaginae-Gardnerella biofilms, confirming the potential to be used for the detection of BV-associated pathogens.Lúcia G. V. SousaJoana CastroAngela FrançaCarina AlmeidaCarina AlmeidaChristina A. MuznyNuno CercaFrontiers Media S.A.articleFannyhessea vaginaeGardnerella vaginalisbacterial vaginosisfluorescence in situ hybridization (FISH)peptide nucleic acid (PNA)MicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fannyhessea vaginae Gardnerella vaginalis bacterial vaginosis fluorescence in situ hybridization (FISH) peptide nucleic acid (PNA) Microbiology QR1-502
spellingShingle	Fannyhessea vaginae Gardnerella vaginalis bacterial vaginosis fluorescence in situ hybridization (FISH) peptide nucleic acid (PNA) Microbiology QR1-502 Lúcia G. V. Sousa Joana Castro Angela França Carina Almeida Carina Almeida Christina A. Muzny Nuno Cerca A New PNA-FISH Probe Targeting Fannyhessea vaginae
description	Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood. Gardnerella spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless, Fannyhessea vaginae (previously known as Atopobium vaginae) is a highly specific species for BV, and co-colonization with Gardnerella is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting F. vaginae. This probe was further validated in a multiplex assay, which included a Gardnerella-specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate F. vaginae-Gardnerella biofilms, confirming the potential to be used for the detection of BV-associated pathogens.
format	article
author	Lúcia G. V. Sousa Joana Castro Angela França Carina Almeida Carina Almeida Christina A. Muzny Nuno Cerca
author_facet	Lúcia G. V. Sousa Joana Castro Angela França Carina Almeida Carina Almeida Christina A. Muzny Nuno Cerca
author_sort	Lúcia G. V. Sousa
title	A New PNA-FISH Probe Targeting Fannyhessea vaginae
title_short	A New PNA-FISH Probe Targeting Fannyhessea vaginae
title_full	A New PNA-FISH Probe Targeting Fannyhessea vaginae
title_fullStr	A New PNA-FISH Probe Targeting Fannyhessea vaginae
title_full_unstemmed	A New PNA-FISH Probe Targeting Fannyhessea vaginae
title_sort	new pna-fish probe targeting fannyhessea vaginae
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/56ac04350bad4a72b65af79ab6352dac
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A New PNA-FISH Probe Targeting Fannyhessea vaginae

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