Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance

Yu Xia, Yanxia Zhang, Huiyun Wang Department of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, Weifang, Shandong, People’s Republic of ChinaCorrespondence: Yu XiaDepartment of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, No. 4138, Linglongshan Road, Weifang,...

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Autores principales:	Xia Y, Zhang Y, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	hcg18 mir-197-3p diagnosis insulin resistance lipid accumulation Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/56c372f1bd264b328f5a870aa989e53b
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spelling	oai:doaj.org-article:56c372f1bd264b328f5a870aa989e53b2021-12-02T19:17:36ZUpregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance1178-7007https://doaj.org/article/56c372f1bd264b328f5a870aa989e53b2021-12-01T00:00:00Zhttps://www.dovepress.com/upregulated-lncrna-hcg18-in-patients-with-non-alcoholic-fatty-liver-di-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Yu Xia, Yanxia Zhang, Huiyun Wang Department of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, Weifang, Shandong, People’s Republic of ChinaCorrespondence: Yu XiaDepartment of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, No. 4138, Linglongshan Road, Weifang, Shandong, 262500, People’s Republic of ChinaTel +86-536-3279993Email xiayuvsrain@163.comPurpose: Non-alcoholic fatty liver disease (NAFLD) is a disease associated with genetic-environmental-metabolic stress, which severely damages the liver function of patients. This study aimed to explore the significance and probable functions of HCG18 in NAFLD.Patients and Methods: The expression of HCG18 and miR-197-3p was tested by qRT-PCR. The clinical signification of HCG18 was provided by the ROC curve and Pearson correlation. The corresponding mechanism was punctuated by the luciferase reporter assay and HFD-managed mice.Results: HCG18 expression was higher in the patients with NAFLD than in controls and in individuals with HOMA-IR score ≥ 2.5 than those with HOMA-IR score < 2.5. HCG18 expression in NAFLD patients was related to BMI, HOMA-IR, ALT, FBG, TC, and TG. HCG18 showed satisfactory predictive accuracy in differentiating NAFLD patients and patients with HOMA-IR ≥ 2.5. Besides, HCG18 had protective impacts on blood glucose and fat deposition but not on body weight. MiR-197-3p is a direct gene of HCG18, and a reverse correlation was found between miR-197-3p and HCG18. Furthermore, miR-197-3p regulated the influence of HCG18 on insulin resistance and lipid accumulation.Conclusion: Increased levels of HCG18 might be an alternate indicator for NAFLD patients. The HCG18-miR-197-3p axis exerted effects on the progression of fat sedimentation and glucose disorder in NAFLD.Keywords: HCG18 miR-197-3p, diagnosis, insulin resistance, lipid accumulationXia YZhang YWang HDove Medical Pressarticlehcg18 mir-197-3pdiagnosisinsulin resistancelipid accumulationSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 4747-4756 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hcg18 mir-197-3p diagnosis insulin resistance lipid accumulation Specialties of internal medicine RC581-951
spellingShingle	hcg18 mir-197-3p diagnosis insulin resistance lipid accumulation Specialties of internal medicine RC581-951 Xia Y Zhang Y Wang H Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
description	Yu Xia, Yanxia Zhang, Huiyun Wang Department of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, Weifang, Shandong, People’s Republic of ChinaCorrespondence: Yu XiaDepartment of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, No. 4138, Linglongshan Road, Weifang, Shandong, 262500, People’s Republic of ChinaTel +86-536-3279993Email xiayuvsrain@163.comPurpose: Non-alcoholic fatty liver disease (NAFLD) is a disease associated with genetic-environmental-metabolic stress, which severely damages the liver function of patients. This study aimed to explore the significance and probable functions of HCG18 in NAFLD.Patients and Methods: The expression of HCG18 and miR-197-3p was tested by qRT-PCR. The clinical signification of HCG18 was provided by the ROC curve and Pearson correlation. The corresponding mechanism was punctuated by the luciferase reporter assay and HFD-managed mice.Results: HCG18 expression was higher in the patients with NAFLD than in controls and in individuals with HOMA-IR score ≥ 2.5 than those with HOMA-IR score < 2.5. HCG18 expression in NAFLD patients was related to BMI, HOMA-IR, ALT, FBG, TC, and TG. HCG18 showed satisfactory predictive accuracy in differentiating NAFLD patients and patients with HOMA-IR ≥ 2.5. Besides, HCG18 had protective impacts on blood glucose and fat deposition but not on body weight. MiR-197-3p is a direct gene of HCG18, and a reverse correlation was found between miR-197-3p and HCG18. Furthermore, miR-197-3p regulated the influence of HCG18 on insulin resistance and lipid accumulation.Conclusion: Increased levels of HCG18 might be an alternate indicator for NAFLD patients. The HCG18-miR-197-3p axis exerted effects on the progression of fat sedimentation and glucose disorder in NAFLD.Keywords: HCG18 miR-197-3p, diagnosis, insulin resistance, lipid accumulation
format	article
author	Xia Y Zhang Y Wang H
author_facet	Xia Y Zhang Y Wang H
author_sort	Xia Y
title	Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
title_short	Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
title_full	Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
title_fullStr	Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
title_full_unstemmed	Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance
title_sort	upregulated lncrna hcg18 in patients with non-alcoholic fatty liver disease and its regulatory effect on insulin resistance
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/56c372f1bd264b328f5a870aa989e53b
work_keys_str_mv	AT xiay upregulatedlncrnahcg18inpatientswithnonalcoholicfattyliverdiseaseanditsregulatoryeffectoninsulinresistance AT zhangy upregulatedlncrnahcg18inpatientswithnonalcoholicfattyliverdiseaseanditsregulatoryeffectoninsulinresistance AT wangh upregulatedlncrnahcg18inpatientswithnonalcoholicfattyliverdiseaseanditsregulatoryeffectoninsulinresistance
_version_	1718376851482607616

Upregulated lncRNA HCG18 in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance

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