Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels

Background: Many of plant-derived compounds inhibiting nerve conduction enhance glutamatergic spontaneous excitatory transmission by activating transient receptor potential (TRP) channels in spinal substantia gelatinosa (SG) neurons that play a crucial role in regulating nociceptive transmission. Al...

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Autores principales:	Chong Wang, Tsugumi Fujita, Hiroki Yasuda, Eiichi Kumamoto
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Linalool Geraniol Transient receptor potential channel Spinal dorsal horn Spontaneous excitatory transmission Membrane hyperpolarization Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/56c7ad4611a84f69a07753915c4d4a32
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spelling	oai:doaj.org-article:56c7ad4611a84f69a07753915c4d4a322021-11-24T04:35:26ZSpontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels2667-031310.1016/j.phyplu.2021.100155https://doaj.org/article/56c7ad4611a84f69a07753915c4d4a322022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667031321001378https://doaj.org/toc/2667-0313Background: Many of plant-derived compounds inhibiting nerve conduction enhance glutamatergic spontaneous excitatory transmission by activating transient receptor potential (TRP) channels in spinal substantia gelatinosa (SG) neurons that play a crucial role in regulating nociceptive transmission. Although (±)-linalool and its isomer geraniol having antinociceptive effects inhibit nerve conduction, it has not been examined yet how they affect excitatory transmission in SG neurons. We examined the effects of the compounds on action potential-independent spontaneous excitatory transmission with a focus on an involvement of TRP channels. Methods: The whole-cell patch-clamp technique was applied to SG neurons in adult rat spinal cord slices. Results: (±)-Linalool increased the frequency of spontaneous excitatory postsynaptic current (sEPSC) with a small increase in its amplitude. The (±)-linalool activity was sensitive to TRP vanilloid-1 (TRPV1) antagonist capsazepine and TRP ankyrin-1 (TRPA1) antagonist HC-030,031 while resistant to BCTC that is antagonist for cloned TRPV1 and TRP melastatin-8 (TRPM8) channels and for TRPM8 channels in the SG, indicating TRPV1 and TRPA1 activation. In 73% of the neurons tested, (±)-linalool produced an outward current at -70 mV. In SG neurons sensitive to (±)-linalool, geraniol produced a quantitatively similar effect to (±)-linalool. Geraniol-induced sEPSC frequency increase was sensitive to BCTC but resistant to capsazepine and HC-030,031, indicating TRPM8 activation. Outward currents produced by (±)-linalool and geraniol were unaffected by all of the TRP antagonists. Conclusion: In SG neurons, (±)-linalool and geraniol presynaptically enhanced spontaneous excitatory transmission by activating different types of TRP channel while hyperpolarizing membranes in a manner independent of TRP channels. Such a TRP channel modulation and hyperpolarization could contribute to the antinociceptive effects of (±)-linalool and geraniol.Chong WangTsugumi FujitaHiroki YasudaEiichi KumamotoElsevierarticleLinaloolGeraniolTransient receptor potential channelSpinal dorsal hornSpontaneous excitatory transmissionMembrane hyperpolarizationOther systems of medicineRZ201-999ENPhytomedicine Plus, Vol 2, Iss 1, Pp 100155- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Linalool Geraniol Transient receptor potential channel Spinal dorsal horn Spontaneous excitatory transmission Membrane hyperpolarization Other systems of medicine RZ201-999
spellingShingle	Linalool Geraniol Transient receptor potential channel Spinal dorsal horn Spontaneous excitatory transmission Membrane hyperpolarization Other systems of medicine RZ201-999 Chong Wang Tsugumi Fujita Hiroki Yasuda Eiichi Kumamoto Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
description	Background: Many of plant-derived compounds inhibiting nerve conduction enhance glutamatergic spontaneous excitatory transmission by activating transient receptor potential (TRP) channels in spinal substantia gelatinosa (SG) neurons that play a crucial role in regulating nociceptive transmission. Although (±)-linalool and its isomer geraniol having antinociceptive effects inhibit nerve conduction, it has not been examined yet how they affect excitatory transmission in SG neurons. We examined the effects of the compounds on action potential-independent spontaneous excitatory transmission with a focus on an involvement of TRP channels. Methods: The whole-cell patch-clamp technique was applied to SG neurons in adult rat spinal cord slices. Results: (±)-Linalool increased the frequency of spontaneous excitatory postsynaptic current (sEPSC) with a small increase in its amplitude. The (±)-linalool activity was sensitive to TRP vanilloid-1 (TRPV1) antagonist capsazepine and TRP ankyrin-1 (TRPA1) antagonist HC-030,031 while resistant to BCTC that is antagonist for cloned TRPV1 and TRP melastatin-8 (TRPM8) channels and for TRPM8 channels in the SG, indicating TRPV1 and TRPA1 activation. In 73% of the neurons tested, (±)-linalool produced an outward current at -70 mV. In SG neurons sensitive to (±)-linalool, geraniol produced a quantitatively similar effect to (±)-linalool. Geraniol-induced sEPSC frequency increase was sensitive to BCTC but resistant to capsazepine and HC-030,031, indicating TRPM8 activation. Outward currents produced by (±)-linalool and geraniol were unaffected by all of the TRP antagonists. Conclusion: In SG neurons, (±)-linalool and geraniol presynaptically enhanced spontaneous excitatory transmission by activating different types of TRP channel while hyperpolarizing membranes in a manner independent of TRP channels. Such a TRP channel modulation and hyperpolarization could contribute to the antinociceptive effects of (±)-linalool and geraniol.
format	article
author	Chong Wang Tsugumi Fujita Hiroki Yasuda Eiichi Kumamoto
author_facet	Chong Wang Tsugumi Fujita Hiroki Yasuda Eiichi Kumamoto
author_sort	Chong Wang
title	Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
title_short	Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
title_full	Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
title_fullStr	Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
title_full_unstemmed	Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
title_sort	spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/56c7ad4611a84f69a07753915c4d4a32
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Spontaneous excitatory transmission enhancement produced by linalool and its isomer geraniol in rat spinal substantia gelatinosa neurons - involvement of transient receptor potential channels

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