Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains

Background: Cytochrome P450-dependent monooxygenases are a very important metabolic system involve in insecticide resistance. This study was conducted to find the association between the expression level of cytochrome P450 (CYP450) and permethrin-resistance level among four strains of the German c...

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Autores principales:	Azimeh Ghaderi, Mozghan Baniardalani, Hamid Reza Basseri
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	Cytochrome P450; German cockroach; Insecticide resistance; Permethrin Pathology RB1-214
Acceso en línea:	https://doaj.org/article/56caac154a87445492e441609b859425
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spelling	oai:doaj.org-article:56caac154a87445492e441609b8594252021-12-02T17:09:55ZLevel of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains10.18502/jad.v15i2.74841735-71792322-2271https://doaj.org/article/56caac154a87445492e441609b8594252021-07-01T00:00:00Zhttps://jad.tums.ac.ir/index.php/jad/article/view/1472https://doaj.org/toc/1735-7179https://doaj.org/toc/2322-2271 Background: Cytochrome P450-dependent monooxygenases are a very important metabolic system involve in insecticide resistance. This study was conducted to find the association between the expression level of cytochrome P450 (CYP450) and permethrin-resistance level among four strains of the German cockroach Blattella germanica (L) (Blattodea: Ectobiidae). Methods: Three field strains of German cockroach with different frequency of exposure to pesticides, and a laboratory susceptible strain were used in the present study. Insecticide susceptibility bioassays were carried out to detect resistance to permethrin. The concentration of CYP450 in each strain was determined using ion-exchange HPLC chromatography. Biochemical assays was performed to analyse CYP450 activities. Results: The resistance ratios (RR) to permethrin among three field strains were 3.29, 4.10 and 6.17-fold comping with the susceptible stain. The CYP450 activity of three field strains was 1.6, 2.4 and 2.7 times higher than in the susceptible stain. The amount of CYP450 per mg of protein was significantly different between the susceptible and the three resistant strains. The resistant cockroaches showed a relatively high expression of CYP450 enzymes. A strong correlation was found between permethrin resistance level and total concentration of CYP450 enzymes. Conclusion: The results of current study show that more frequent usage of a pyrethroid insecticide cause the metabolic insecticide resistance to rise in German cockroach. Therefore, there is a ratio correlation between resistance level and monooxygenases activities in insect. Thus, the control program must be managed according to history of pesticide usage. Azimeh GhaderiMozghan BaniardalaniHamid Reza BasseriTehran University of Medical SciencesarticleCytochrome P450; German cockroach; Insecticide resistance; PermethrinPathologyRB1-214ENJournal of Arthropod-Borne Diseases , Vol 15, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytochrome P450; German cockroach; Insecticide resistance; Permethrin Pathology RB1-214
spellingShingle	Cytochrome P450; German cockroach; Insecticide resistance; Permethrin Pathology RB1-214 Azimeh Ghaderi Mozghan Baniardalani Hamid Reza Basseri Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
description	Background: Cytochrome P450-dependent monooxygenases are a very important metabolic system involve in insecticide resistance. This study was conducted to find the association between the expression level of cytochrome P450 (CYP450) and permethrin-resistance level among four strains of the German cockroach Blattella germanica (L) (Blattodea: Ectobiidae). Methods: Three field strains of German cockroach with different frequency of exposure to pesticides, and a laboratory susceptible strain were used in the present study. Insecticide susceptibility bioassays were carried out to detect resistance to permethrin. The concentration of CYP450 in each strain was determined using ion-exchange HPLC chromatography. Biochemical assays was performed to analyse CYP450 activities. Results: The resistance ratios (RR) to permethrin among three field strains were 3.29, 4.10 and 6.17-fold comping with the susceptible stain. The CYP450 activity of three field strains was 1.6, 2.4 and 2.7 times higher than in the susceptible stain. The amount of CYP450 per mg of protein was significantly different between the susceptible and the three resistant strains. The resistant cockroaches showed a relatively high expression of CYP450 enzymes. A strong correlation was found between permethrin resistance level and total concentration of CYP450 enzymes. Conclusion: The results of current study show that more frequent usage of a pyrethroid insecticide cause the metabolic insecticide resistance to rise in German cockroach. Therefore, there is a ratio correlation between resistance level and monooxygenases activities in insect. Thus, the control program must be managed according to history of pesticide usage.
format	article
author	Azimeh Ghaderi Mozghan Baniardalani Hamid Reza Basseri
author_facet	Azimeh Ghaderi Mozghan Baniardalani Hamid Reza Basseri
author_sort	Azimeh Ghaderi
title	Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
title_short	Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
title_full	Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
title_fullStr	Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
title_full_unstemmed	Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains
title_sort	level of pyrethroid-resistance associated with cytochrome p450 expression in german cockroach blattella germanica (blattodea: ectobiidae) in the field collected strains
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/56caac154a87445492e441609b859425
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Level of Pyrethroid-resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains

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