Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy

Background: Galectin-3 (Gal-3) is a multifunctional glycan-binding protein shown to be linked to chronic inflammation and fibrogenesis. Plasma Gal-3 is associated with proteinuria and renal dysfunction, but its role has never been confirmed with kidney biopsy results. In our study, we aimed to explo...

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Autores principales:	Shuo-Ming Ou, Ming-Tsun Tsai, Huan-Yuan Chen, Fu-An Li, Wei-Cheng Tseng, Kuo-Hua Lee, Fu-Pang Chang, Yao-Ping Lin, Ruey-Bing Yang, Der-Cherng Tarng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	galectin-3 chronic kidney disease tubular atrophy interstitial fibrosis kidney biopsy RNA-sequencing analysis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/56cb477fab1946c390ed0f1710c1d258
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spelling	oai:doaj.org-article:56cb477fab1946c390ed0f1710c1d2582021-11-12T06:50:44ZIdentification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy2296-858X10.3389/fmed.2021.748225https://doaj.org/article/56cb477fab1946c390ed0f1710c1d2582021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.748225/fullhttps://doaj.org/toc/2296-858XBackground: Galectin-3 (Gal-3) is a multifunctional glycan-binding protein shown to be linked to chronic inflammation and fibrogenesis. Plasma Gal-3 is associated with proteinuria and renal dysfunction, but its role has never been confirmed with kidney biopsy results. In our study, we aimed to explore the expression of Gal-3 in biopsy-proven patients, and we tested the hypothesis that chronic kidney disease (CKD) leads to upregulation of plasma Gal-3 expression in corresponding biopsy findings and RNA sequencing analysis.Method: In 249 patients (male/female: 155/94, age: 57.2 ± 16.3 years) who underwent kidney biopsy, plasma levels of Gal-3 were measured to estimate the association of renal fibrosis. Relationships between plasma Gal-3 levels, estimated glomerular filtration rate (eGFR) and renal histology findings were also assessed. We further examined the gene expression of Gal-3 in RNA-sequencing analysis in biopsy-proven patients.Results: Compared to patients without CKD, CKD patients had higher levels of plasma Gal-3 (1,016.3 ± 628.1 pg/mL vs. 811.6 ± 369.6 pg/ml; P = 0.010). Plasma Gal-3 was inversely correlated with eGFR (P = 0.005) but not with proteinuria. Higher Gal-3 levels were associated with interstitial fibrosis, tubular atrophy and vascular intimal fibrosis. RNA-sequencing analysis showed the upregulation of Gal-3 in fibrotic kidney biopsy samples, and the differentially expressed genes were mainly enhanced in immune cell activation and the regulation of cell-cell adhesion.Conclusions: Plasma Gal-3 levels are inverse correlated with eGFR but positively correlated with renal fibrosis, which may be involved in the immune response and associated pathways. These findings support the role of Gal-3 as a predictive marker of renal fibrosis.Shuo-Ming OuShuo-Ming OuShuo-Ming OuShuo-Ming OuShuo-Ming OuShuo-Ming OuMing-Tsun TsaiMing-Tsun TsaiMing-Tsun TsaiMing-Tsun TsaiMing-Tsun TsaiMing-Tsun TsaiHuan-Yuan ChenFu-An LiWei-Cheng TsengWei-Cheng TsengWei-Cheng TsengWei-Cheng TsengWei-Cheng TsengWei-Cheng TsengKuo-Hua LeeKuo-Hua LeeKuo-Hua LeeKuo-Hua LeeKuo-Hua LeeKuo-Hua LeeFu-Pang ChangFu-Pang ChangYao-Ping LinYao-Ping LinYao-Ping LinYao-Ping LinYao-Ping LinYao-Ping LinRuey-Bing YangDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngDer-Cherng TarngFrontiers Media S.A.articlegalectin-3chronic kidney diseasetubular atrophyinterstitial fibrosiskidney biopsyRNA-sequencing analysisMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	galectin-3 chronic kidney disease tubular atrophy interstitial fibrosis kidney biopsy RNA-sequencing analysis Medicine (General) R5-920
spellingShingle	galectin-3 chronic kidney disease tubular atrophy interstitial fibrosis kidney biopsy RNA-sequencing analysis Medicine (General) R5-920 Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Huan-Yuan Chen Fu-An Li Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Fu-Pang Chang Fu-Pang Chang Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Ruey-Bing Yang Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
description	Background: Galectin-3 (Gal-3) is a multifunctional glycan-binding protein shown to be linked to chronic inflammation and fibrogenesis. Plasma Gal-3 is associated with proteinuria and renal dysfunction, but its role has never been confirmed with kidney biopsy results. In our study, we aimed to explore the expression of Gal-3 in biopsy-proven patients, and we tested the hypothesis that chronic kidney disease (CKD) leads to upregulation of plasma Gal-3 expression in corresponding biopsy findings and RNA sequencing analysis.Method: In 249 patients (male/female: 155/94, age: 57.2 ± 16.3 years) who underwent kidney biopsy, plasma levels of Gal-3 were measured to estimate the association of renal fibrosis. Relationships between plasma Gal-3 levels, estimated glomerular filtration rate (eGFR) and renal histology findings were also assessed. We further examined the gene expression of Gal-3 in RNA-sequencing analysis in biopsy-proven patients.Results: Compared to patients without CKD, CKD patients had higher levels of plasma Gal-3 (1,016.3 ± 628.1 pg/mL vs. 811.6 ± 369.6 pg/ml; P = 0.010). Plasma Gal-3 was inversely correlated with eGFR (P = 0.005) but not with proteinuria. Higher Gal-3 levels were associated with interstitial fibrosis, tubular atrophy and vascular intimal fibrosis. RNA-sequencing analysis showed the upregulation of Gal-3 in fibrotic kidney biopsy samples, and the differentially expressed genes were mainly enhanced in immune cell activation and the regulation of cell-cell adhesion.Conclusions: Plasma Gal-3 levels are inverse correlated with eGFR but positively correlated with renal fibrosis, which may be involved in the immune response and associated pathways. These findings support the role of Gal-3 as a predictive marker of renal fibrosis.
format	article
author	Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Huan-Yuan Chen Fu-An Li Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Fu-Pang Chang Fu-Pang Chang Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Ruey-Bing Yang Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng
author_facet	Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Shuo-Ming Ou Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Ming-Tsun Tsai Huan-Yuan Chen Fu-An Li Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Wei-Cheng Tseng Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Kuo-Hua Lee Fu-Pang Chang Fu-Pang Chang Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Yao-Ping Lin Ruey-Bing Yang Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng Der-Cherng Tarng
author_sort	Shuo-Ming Ou
title	Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
title_short	Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
title_full	Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
title_fullStr	Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
title_full_unstemmed	Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy
title_sort	identification of galectin-3 as potential biomarkers for renal fibrosis by rna-sequencing and clinicopathologic findings of kidney biopsy
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/56cb477fab1946c390ed0f1710c1d258
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Identification of Galectin-3 as Potential Biomarkers for Renal Fibrosis by RNA-Sequencing and Clinicopathologic Findings of Kidney Biopsy

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