Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth

Abstract Mushrooms are the most conspicuous fungal structures. Transcription factors (TFs) Bri1 and Hom1 of the model fungus Schizophyllum commune are involved in late stages of mushroom development, while Wc-2, Hom2, and Fst4 function early in development. Here, it is shown that Bri1 and Hom1 also...

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Autores principales:	Jordi F. Pelkmans, Mohini B. Patil, Thies Gehrmann, Marcel J. T. Reinders, Han A. B. Wösten, Luis G. Lugones
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56cc077c1cc14608817652411e4e29eb
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spelling	oai:doaj.org-article:56cc077c1cc14608817652411e4e29eb2021-12-02T15:06:25ZTranscription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth10.1038/s41598-017-00483-32045-2322https://doaj.org/article/56cc077c1cc14608817652411e4e29eb2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00483-3https://doaj.org/toc/2045-2322Abstract Mushrooms are the most conspicuous fungal structures. Transcription factors (TFs) Bri1 and Hom1 of the model fungus Schizophyllum commune are involved in late stages of mushroom development, while Wc-2, Hom2, and Fst4 function early in development. Here, it is shown that Bri1 and Hom1 also stimulate vegetative growth, while biomass formation is repressed by Wc-2, Hom2, and Fst4. The Δbri1Δbri1 and the Δhom1Δhom1 strains formed up to 0.6 fold less biomass when compared to wild-type, while Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains formed up to 2.8 fold more biomass. Inactivation of TF gene tea1, which was downregulated in the Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains, resulted in a strain that was severely affected in mushroom development and that produced 1.3 fold more biomass than the wild-type. In contrast, introducing a constitutive active version of hom2 that had 4 predicted phosphorylation motifs eliminated resulted in radial growth inhibition and prompt fructification in both Δhom2 and wild-type strains, even in sterile monokaryons. Together, it is concluded that TFs involved in mushroom formation also modulate vegetative growth. Among these TFs is the homeodomain protein Hom2, being the first time that this class of regulatory proteins is implicated in repression of vegetative growth in a eukaryote.Jordi F. PelkmansMohini B. PatilThies GehrmannMarcel J. T. ReindersHan A. B. WöstenLuis G. LugonesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jordi F. Pelkmans Mohini B. Patil Thies Gehrmann Marcel J. T. Reinders Han A. B. Wösten Luis G. Lugones Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
description	Abstract Mushrooms are the most conspicuous fungal structures. Transcription factors (TFs) Bri1 and Hom1 of the model fungus Schizophyllum commune are involved in late stages of mushroom development, while Wc-2, Hom2, and Fst4 function early in development. Here, it is shown that Bri1 and Hom1 also stimulate vegetative growth, while biomass formation is repressed by Wc-2, Hom2, and Fst4. The Δbri1Δbri1 and the Δhom1Δhom1 strains formed up to 0.6 fold less biomass when compared to wild-type, while Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains formed up to 2.8 fold more biomass. Inactivation of TF gene tea1, which was downregulated in the Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains, resulted in a strain that was severely affected in mushroom development and that produced 1.3 fold more biomass than the wild-type. In contrast, introducing a constitutive active version of hom2 that had 4 predicted phosphorylation motifs eliminated resulted in radial growth inhibition and prompt fructification in both Δhom2 and wild-type strains, even in sterile monokaryons. Together, it is concluded that TFs involved in mushroom formation also modulate vegetative growth. Among these TFs is the homeodomain protein Hom2, being the first time that this class of regulatory proteins is implicated in repression of vegetative growth in a eukaryote.
format	article
author	Jordi F. Pelkmans Mohini B. Patil Thies Gehrmann Marcel J. T. Reinders Han A. B. Wösten Luis G. Lugones
author_facet	Jordi F. Pelkmans Mohini B. Patil Thies Gehrmann Marcel J. T. Reinders Han A. B. Wösten Luis G. Lugones
author_sort	Jordi F. Pelkmans
title	Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
title_short	Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
title_full	Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
title_fullStr	Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
title_full_unstemmed	Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
title_sort	transcription factors of schizophyllum commune involved in mushroom formation and modulation of vegetative growth
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/56cc077c1cc14608817652411e4e29eb
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Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth

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