Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma

Abstract Aberrant DNA modifications affect the tumorigenesis and progression of lung cancer. However, the global methylation status of tumor cells and the heterogeneous methylation status of cells within the same tumor need further study. We used publicly available single-cell RNAseq data to investi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Quan-Fang Chen, Han Gao, Qing-Yun Pan, Ying-Ju Wang, Xiao-Ning Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56ccb5b3517e4e4187a760697307ad60
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:56ccb5b3517e4e4187a760697307ad60
record_format	dspace
spelling	oai:doaj.org-article:56ccb5b3517e4e4187a760697307ad602021-12-05T12:14:49ZAnalysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma10.1038/s41598-021-02786-y2045-2322https://doaj.org/article/56ccb5b3517e4e4187a760697307ad602021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02786-yhttps://doaj.org/toc/2045-2322Abstract Aberrant DNA modifications affect the tumorigenesis and progression of lung cancer. However, the global methylation status of tumor cells and the heterogeneous methylation status of cells within the same tumor need further study. We used publicly available single-cell RNAseq data to investigate the impact and diversity of global methylation status on lung adenocarcinoma. Clustering cells into subgroups and cell differentiation pseudotime analysis, based on expression profile, demonstrated that the global methylation status was crucial to lung adenocarcinoma function and progression. Hypermethylated tumor cells had increased activity related to the hypoxia response. Hyper- and hypomethylated cells indicated upregulation in pathways involving focal adhesion and cell junctions. Pseudotime analysis showed that cell clusters with unique methylation activities were located at the ends of the putative trajectories, suggesting that DNA methylation and demethylation activities were essential to tumor cell progression. Expression of SPP1 was associated with the global methylation status of tumor cells and with patient prognosis. Our study identified the importance and diversity of global DNA methylation status by analysis at the single-cell level. Our findings provide new information about the global DNA methylation status of tumor cells and suggest new approaches for precision medical treatments for lung adenocarcinoma.Quan-Fang ChenHan GaoQing-Yun PanYing-Ju WangXiao-Ning ZhongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quan-Fang Chen Han Gao Qing-Yun Pan Ying-Ju Wang Xiao-Ning Zhong Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
description	Abstract Aberrant DNA modifications affect the tumorigenesis and progression of lung cancer. However, the global methylation status of tumor cells and the heterogeneous methylation status of cells within the same tumor need further study. We used publicly available single-cell RNAseq data to investigate the impact and diversity of global methylation status on lung adenocarcinoma. Clustering cells into subgroups and cell differentiation pseudotime analysis, based on expression profile, demonstrated that the global methylation status was crucial to lung adenocarcinoma function and progression. Hypermethylated tumor cells had increased activity related to the hypoxia response. Hyper- and hypomethylated cells indicated upregulation in pathways involving focal adhesion and cell junctions. Pseudotime analysis showed that cell clusters with unique methylation activities were located at the ends of the putative trajectories, suggesting that DNA methylation and demethylation activities were essential to tumor cell progression. Expression of SPP1 was associated with the global methylation status of tumor cells and with patient prognosis. Our study identified the importance and diversity of global DNA methylation status by analysis at the single-cell level. Our findings provide new information about the global DNA methylation status of tumor cells and suggest new approaches for precision medical treatments for lung adenocarcinoma.
format	article
author	Quan-Fang Chen Han Gao Qing-Yun Pan Ying-Ju Wang Xiao-Ning Zhong
author_facet	Quan-Fang Chen Han Gao Qing-Yun Pan Ying-Ju Wang Xiao-Ning Zhong
author_sort	Quan-Fang Chen
title	Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
title_short	Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
title_full	Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
title_fullStr	Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
title_full_unstemmed	Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma
title_sort	analysis at the single-cell level indicates an important role of heterogeneous global dna methylation status on the progression of lung adenocarcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/56ccb5b3517e4e4187a760697307ad60
work_keys_str_mv	AT quanfangchen analysisatthesinglecelllevelindicatesanimportantroleofheterogeneousglobaldnamethylationstatusontheprogressionoflungadenocarcinoma AT hangao analysisatthesinglecelllevelindicatesanimportantroleofheterogeneousglobaldnamethylationstatusontheprogressionoflungadenocarcinoma AT qingyunpan analysisatthesinglecelllevelindicatesanimportantroleofheterogeneousglobaldnamethylationstatusontheprogressionoflungadenocarcinoma AT yingjuwang analysisatthesinglecelllevelindicatesanimportantroleofheterogeneousglobaldnamethylationstatusontheprogressionoflungadenocarcinoma AT xiaoningzhong analysisatthesinglecelllevelindicatesanimportantroleofheterogeneousglobaldnamethylationstatusontheprogressionoflungadenocarcinoma
_version_	1718372133089837056

Analysis at the single-cell level indicates an important role of heterogeneous global DNA methylation status on the progression of lung adenocarcinoma

Ejemplares similares