The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy

Abstract Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation i...

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Autores principales:	Maren Anna Brandsrud, Reinhold Blümel, Johanne Heitmann Solheim, Achim Kohler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56cf3381ffd9411aac3309c9faabaaea
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spelling	oai:doaj.org-article:56cf3381ffd9411aac3309c9faabaaea2021-12-02T13:35:05ZThe effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy10.1038/s41598-021-84064-52045-2322https://doaj.org/article/56cf3381ffd9411aac3309c9faabaaea2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84064-5https://doaj.org/toc/2045-2322Abstract Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation is of the same order as the size of the scatterer. By use of approximations to the Mie solutions for spheres, iterative algorithms have been developed to retrieve pure absorbance spectra. However, the question remains to what extent the Mie solutions, and approximations thereof, describe the extinction efficiency in practical situations where the shapes of scatterers deviate considerably from spheres. The aim of the current study is to investigate how deviations from a spherical scatterer can change the extinction properties of the scatterer in the context of chaos in wave systems. For this purpose, we investigate a chaotic scatterer and compare it with an elliptically shaped scatterer, which exhibits only regular scattering. We find that chaotic scattering has an accelerating effect on the disappearance of Mie ripples. We further show that the presence of absorption and the high numerical aperture of infrared microscopes does not explain the absence of ripples in most measurements of biological samples.Maren Anna BrandsrudReinhold BlümelJohanne Heitmann SolheimAchim KohlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maren Anna Brandsrud Reinhold Blümel Johanne Heitmann Solheim Achim Kohler The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
description	Abstract Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation is of the same order as the size of the scatterer. By use of approximations to the Mie solutions for spheres, iterative algorithms have been developed to retrieve pure absorbance spectra. However, the question remains to what extent the Mie solutions, and approximations thereof, describe the extinction efficiency in practical situations where the shapes of scatterers deviate considerably from spheres. The aim of the current study is to investigate how deviations from a spherical scatterer can change the extinction properties of the scatterer in the context of chaos in wave systems. For this purpose, we investigate a chaotic scatterer and compare it with an elliptically shaped scatterer, which exhibits only regular scattering. We find that chaotic scattering has an accelerating effect on the disappearance of Mie ripples. We further show that the presence of absorption and the high numerical aperture of infrared microscopes does not explain the absence of ripples in most measurements of biological samples.
format	article
author	Maren Anna Brandsrud Reinhold Blümel Johanne Heitmann Solheim Achim Kohler
author_facet	Maren Anna Brandsrud Reinhold Blümel Johanne Heitmann Solheim Achim Kohler
author_sort	Maren Anna Brandsrud
title	The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
title_short	The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
title_full	The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
title_fullStr	The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
title_full_unstemmed	The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
title_sort	effect of deformation of absorbing scatterers on mie-type signatures in infrared microspectroscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/56cf3381ffd9411aac3309c9faabaaea
work_keys_str_mv	AT marenannabrandsrud theeffectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT reinholdblumel theeffectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT johanneheitmannsolheim theeffectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT achimkohler theeffectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT marenannabrandsrud effectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT reinholdblumel effectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT johanneheitmannsolheim effectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy AT achimkohler effectofdeformationofabsorbingscatterersonmietypesignaturesininfraredmicrospectroscopy
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The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy

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