Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>

ABSTRACT Metabolic turnover of mRNA is fundamental to the control of gene expression in all organisms, notably in fast-adapting prokaryotes. In many bacteria, RNase Y initiates global mRNA decay via an endonucleolytic cleavage, as shown in the Gram-positive model organism Bacillus subtilis. This enz...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lina Hamouche, Cyrille Billaudeau, Anna Rocca, Arnaud Chastanet, Saravuth Ngo, Soumaya Laalami, Harald Putzer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Bacillus subtilis RNA degradation RNA processing RNase Y membrane proteins Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/56d807b040944a519243fead5a93f385
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:56d807b040944a519243fead5a93f385
record_format	dspace
spelling	oai:doaj.org-article:56d807b040944a519243fead5a93f3852021-11-15T15:56:58ZDynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>10.1128/mBio.03337-192150-7511https://doaj.org/article/56d807b040944a519243fead5a93f3852020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.03337-19https://doaj.org/toc/2150-7511ABSTRACT Metabolic turnover of mRNA is fundamental to the control of gene expression in all organisms, notably in fast-adapting prokaryotes. In many bacteria, RNase Y initiates global mRNA decay via an endonucleolytic cleavage, as shown in the Gram-positive model organism Bacillus subtilis. This enzyme is tethered to the inner cell membrane, a pseudocompartmentalization coherent with its task of initiating mRNA cleavage/maturation of mRNAs that are translated at the cell periphery. Here, we used total internal reflection fluorescence microscopy (TIRFm) and single-particle tracking (SPT) to visualize RNase Y and analyze its distribution and dynamics in living cells. We find that RNase Y diffuses rapidly at the membrane in the form of dynamic short-lived foci. Unlike RNase E, the major decay-initiating RNase in Escherichia coli, the formation of foci is not dependent on the presence of RNA substrates. On the contrary, RNase Y foci become more abundant and increase in size following transcription arrest, suggesting that they do not constitute the most active form of the nuclease. The Y-complex of three proteins (YaaT, YlbF, and YmcA) has previously been shown to play an important role for RNase Y activity in vivo. We demonstrate that Y-complex mutations have an effect similar to but much stronger than that of depletion of RNA in increasing the number and size of RNase Y foci at the membrane. Our data suggest that the Y-complex shifts the assembly status of RNase Y toward fewer and smaller complexes, thereby increasing cleavage efficiency of complex substrates like polycistronic mRNAs. IMPORTANCE All living organisms must degrade mRNA to adapt gene expression to changing environments. In bacteria, initiation of mRNA decay generally occurs through an endonucleolytic cleavage. In the Gram-positive model organism Bacillus subtilis and probably many other bacteria, the key enzyme for this task is RNase Y, which is anchored at the inner cell membrane. While this pseudocompartmentalization appears coherent with translation occurring primarily at the cell periphery, our knowledge on the distribution and dynamics of RNase Y in living cells is very scarce. Here, we show that RNase Y moves rapidly along the membrane in the form of dynamic short-lived foci. These foci become more abundant and increase in size following transcription arrest, suggesting that they do not constitute the most active form of the nuclease. This contrasts with RNase E, the major decay-initiating RNase in E. coli, where it was shown that formation of foci is dependent on the presence of RNA substrates. We also show that a protein complex (Y-complex) known to influence the specificity of RNase Y activity in vivo is capable of shifting the assembly status of RNase Y toward fewer and smaller complexes. This highlights fundamental differences between RNase E- and RNase Y-based degradation machineries.Lina HamoucheCyrille BillaudeauAnna RoccaArnaud ChastanetSaravuth NgoSoumaya LaalamiHarald PutzerAmerican Society for MicrobiologyarticleBacillus subtilisRNA degradationRNA processingRNase Ymembrane proteinsMicrobiologyQR1-502ENmBio, Vol 11, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus subtilis RNA degradation RNA processing RNase Y membrane proteins Microbiology QR1-502
spellingShingle	Bacillus subtilis RNA degradation RNA processing RNase Y membrane proteins Microbiology QR1-502 Lina Hamouche Cyrille Billaudeau Anna Rocca Arnaud Chastanet Saravuth Ngo Soumaya Laalami Harald Putzer Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
description	ABSTRACT Metabolic turnover of mRNA is fundamental to the control of gene expression in all organisms, notably in fast-adapting prokaryotes. In many bacteria, RNase Y initiates global mRNA decay via an endonucleolytic cleavage, as shown in the Gram-positive model organism Bacillus subtilis. This enzyme is tethered to the inner cell membrane, a pseudocompartmentalization coherent with its task of initiating mRNA cleavage/maturation of mRNAs that are translated at the cell periphery. Here, we used total internal reflection fluorescence microscopy (TIRFm) and single-particle tracking (SPT) to visualize RNase Y and analyze its distribution and dynamics in living cells. We find that RNase Y diffuses rapidly at the membrane in the form of dynamic short-lived foci. Unlike RNase E, the major decay-initiating RNase in Escherichia coli, the formation of foci is not dependent on the presence of RNA substrates. On the contrary, RNase Y foci become more abundant and increase in size following transcription arrest, suggesting that they do not constitute the most active form of the nuclease. The Y-complex of three proteins (YaaT, YlbF, and YmcA) has previously been shown to play an important role for RNase Y activity in vivo. We demonstrate that Y-complex mutations have an effect similar to but much stronger than that of depletion of RNA in increasing the number and size of RNase Y foci at the membrane. Our data suggest that the Y-complex shifts the assembly status of RNase Y toward fewer and smaller complexes, thereby increasing cleavage efficiency of complex substrates like polycistronic mRNAs. IMPORTANCE All living organisms must degrade mRNA to adapt gene expression to changing environments. In bacteria, initiation of mRNA decay generally occurs through an endonucleolytic cleavage. In the Gram-positive model organism Bacillus subtilis and probably many other bacteria, the key enzyme for this task is RNase Y, which is anchored at the inner cell membrane. While this pseudocompartmentalization appears coherent with translation occurring primarily at the cell periphery, our knowledge on the distribution and dynamics of RNase Y in living cells is very scarce. Here, we show that RNase Y moves rapidly along the membrane in the form of dynamic short-lived foci. These foci become more abundant and increase in size following transcription arrest, suggesting that they do not constitute the most active form of the nuclease. This contrasts with RNase E, the major decay-initiating RNase in E. coli, where it was shown that formation of foci is dependent on the presence of RNA substrates. We also show that a protein complex (Y-complex) known to influence the specificity of RNase Y activity in vivo is capable of shifting the assembly status of RNase Y toward fewer and smaller complexes. This highlights fundamental differences between RNase E- and RNase Y-based degradation machineries.
format	article
author	Lina Hamouche Cyrille Billaudeau Anna Rocca Arnaud Chastanet Saravuth Ngo Soumaya Laalami Harald Putzer
author_facet	Lina Hamouche Cyrille Billaudeau Anna Rocca Arnaud Chastanet Saravuth Ngo Soumaya Laalami Harald Putzer
author_sort	Lina Hamouche
title	Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
title_short	Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
title_full	Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
title_fullStr	Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
title_full_unstemmed	Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>
title_sort	dynamic membrane localization of rnase y in <named-content content-type="genus-species">bacillus subtilis</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/56d807b040944a519243fead5a93f385
work_keys_str_mv	AT linahamouche dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT cyrillebillaudeau dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT annarocca dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT arnaudchastanet dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT saravuthngo dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT soumayalaalami dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent AT haraldputzer dynamicmembranelocalizationofrnaseyinnamedcontentcontenttypegenusspeciesbacillussubtilisnamedcontent
_version_	1718427088485089280

Dynamic Membrane Localization of RNase Y in <named-content content-type="genus-species">Bacillus subtilis</named-content>

Ejemplares similares