Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.

<h4>Background</h4>Intercellular communication can occur via the release of membrane vesicles. Exosomes are nanovesicles released from the endosomal compartment of cells. Depending on their cell of origin and their cargo they can exert different immunoregulatory functions. Recently, fung...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ulf Gehrmann, Khaleda Rahman Qazi, Catharina Johansson, Kjell Hultenby, Maria Karlsson, Lena Lundeberg, Susanne Gabrielsson, Annika Scheynius
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56d8503ebf084446b22665ca3461c8bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:56d8503ebf084446b22665ca3461c8bc
record_format	dspace
spelling	oai:doaj.org-article:56d8503ebf084446b22665ca3461c8bc2021-11-18T06:49:40ZNanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.1932-620310.1371/journal.pone.0021480https://doaj.org/article/56d8503ebf084446b22665ca3461c8bc2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21799736/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Intercellular communication can occur via the release of membrane vesicles. Exosomes are nanovesicles released from the endosomal compartment of cells. Depending on their cell of origin and their cargo they can exert different immunoregulatory functions. Recently, fungi were found to produce extracellular vesicles that can influence host-microbe interactions. The yeast Malassezia sympodialis which belongs to our normal cutaneous microbial flora elicits specific IgE- and T-cell reactivity in approximately 50% of adult patients with atopic eczema (AE). Whether exosomes or other vesicles contribute to the inflammation has not yet been investigated.<h4>Objective</h4>To investigate if M. sympodialis can release nanovesicles and whether they or endogenous exosomes can activate PBMC from AE patients sensitized to M. sympodialis.<h4>Methods</h4>Extracellular nanovesicles isolated from M. sympodialis, co-cultures of M. sympodialis and dendritic cells, and from plasma of patients with AE and healthy controls (HC) were characterised using flow cytometry, sucrose gradient centrifugation, Western blot and electron microscopy. Their ability to stimulate IL-4 and TNF-alpha responses in autologous CD14, CD34 depleted PBMC was determined using ELISPOT and ELISA, respectively.<h4>Results</h4>We show for the first time that M. sympodialis releases extracellular vesicles carrying allergen. These vesicles can induce IL-4 and TNF-α responses with a significantly higher IL-4 production in patients compared to HC. Exosomes from dendritic cell and M. sympodialis co-cultures induced IL-4 and TNF-α responses in autologous CD14, CD34 depleted PBMC of AE patients and HC while plasma exosomes induced TNF-α but not IL-4 in undepleted PBMC.<h4>Conclusions</h4>Extracellular vesicles from M. sympodialis, dendritic cells and plasma can contribute to cytokine responses in CD14, CD34 depleted and undepleted PBMC of AE patients and HC. These novel observations have implications for understanding host-microbe interactions in the pathogenesis of AE.Ulf GehrmannKhaleda Rahman QaziCatharina JohanssonKjell HultenbyMaria KarlssonLena LundebergSusanne GabrielssonAnnika ScheyniusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21480 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulf Gehrmann Khaleda Rahman Qazi Catharina Johansson Kjell Hultenby Maria Karlsson Lena Lundeberg Susanne Gabrielsson Annika Scheynius Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
description	<h4>Background</h4>Intercellular communication can occur via the release of membrane vesicles. Exosomes are nanovesicles released from the endosomal compartment of cells. Depending on their cell of origin and their cargo they can exert different immunoregulatory functions. Recently, fungi were found to produce extracellular vesicles that can influence host-microbe interactions. The yeast Malassezia sympodialis which belongs to our normal cutaneous microbial flora elicits specific IgE- and T-cell reactivity in approximately 50% of adult patients with atopic eczema (AE). Whether exosomes or other vesicles contribute to the inflammation has not yet been investigated.<h4>Objective</h4>To investigate if M. sympodialis can release nanovesicles and whether they or endogenous exosomes can activate PBMC from AE patients sensitized to M. sympodialis.<h4>Methods</h4>Extracellular nanovesicles isolated from M. sympodialis, co-cultures of M. sympodialis and dendritic cells, and from plasma of patients with AE and healthy controls (HC) were characterised using flow cytometry, sucrose gradient centrifugation, Western blot and electron microscopy. Their ability to stimulate IL-4 and TNF-alpha responses in autologous CD14, CD34 depleted PBMC was determined using ELISPOT and ELISA, respectively.<h4>Results</h4>We show for the first time that M. sympodialis releases extracellular vesicles carrying allergen. These vesicles can induce IL-4 and TNF-α responses with a significantly higher IL-4 production in patients compared to HC. Exosomes from dendritic cell and M. sympodialis co-cultures induced IL-4 and TNF-α responses in autologous CD14, CD34 depleted PBMC of AE patients and HC while plasma exosomes induced TNF-α but not IL-4 in undepleted PBMC.<h4>Conclusions</h4>Extracellular vesicles from M. sympodialis, dendritic cells and plasma can contribute to cytokine responses in CD14, CD34 depleted and undepleted PBMC of AE patients and HC. These novel observations have implications for understanding host-microbe interactions in the pathogenesis of AE.
format	article
author	Ulf Gehrmann Khaleda Rahman Qazi Catharina Johansson Kjell Hultenby Maria Karlsson Lena Lundeberg Susanne Gabrielsson Annika Scheynius
author_facet	Ulf Gehrmann Khaleda Rahman Qazi Catharina Johansson Kjell Hultenby Maria Karlsson Lena Lundeberg Susanne Gabrielsson Annika Scheynius
author_sort	Ulf Gehrmann
title	Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
title_short	Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
title_full	Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
title_fullStr	Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
title_full_unstemmed	Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
title_sort	nanovesicles from malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/56d8503ebf084446b22665ca3461c8bc
work_keys_str_mv	AT ulfgehrmann nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT khaledarahmanqazi nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT catharinajohansson nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT kjellhultenby nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT mariakarlsson nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT lenalundeberg nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT susannegabrielsson nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema AT annikascheynius nanovesiclesfrommalasseziasympodialisandhostexosomesinducecytokineresponsesnovelmechanismsforhostmicrobeinteractionsinatopiceczema
_version_	1718424352239648768

Nanovesicles from Malassezia sympodialis and host exosomes induce cytokine responses--novel mechanisms for host-microbe interactions in atopic eczema.

Ejemplares similares