A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis

While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by olig...

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Autores principales:	Matthew J. Amicucci, Eshani Nandita, Ace G. Galermo, Juan Jose Castillo, Siyu Chen, Dayoung Park, Jennifer T. Smilowitz, J. Bruce German, David A. Mills, Carlito B. Lebrilla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/56e858fbf541481aa828e4e37d9df50a
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spelling	oai:doaj.org-article:56e858fbf541481aa828e4e37d9df50a2021-12-02T16:36:37ZA nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis10.1038/s41467-020-17778-12041-1723https://doaj.org/article/56e858fbf541481aa828e4e37d9df50a2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17778-1https://doaj.org/toc/2041-1723While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by oligosaccharide fingerprinting.Matthew J. AmicucciEshani NanditaAce G. GalermoJuan Jose CastilloSiyu ChenDayoung ParkJennifer T. SmilowitzJ. Bruce GermanDavid A. MillsCarlito B. LebrillaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Matthew J. Amicucci Eshani Nandita Ace G. Galermo Juan Jose Castillo Siyu Chen Dayoung Park Jennifer T. Smilowitz J. Bruce German David A. Mills Carlito B. Lebrilla A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
description	While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by oligosaccharide fingerprinting.
format	article
author	Matthew J. Amicucci Eshani Nandita Ace G. Galermo Juan Jose Castillo Siyu Chen Dayoung Park Jennifer T. Smilowitz J. Bruce German David A. Mills Carlito B. Lebrilla
author_facet	Matthew J. Amicucci Eshani Nandita Ace G. Galermo Juan Jose Castillo Siyu Chen Dayoung Park Jennifer T. Smilowitz J. Bruce German David A. Mills Carlito B. Lebrilla
author_sort	Matthew J. Amicucci
title	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
title_short	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
title_full	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
title_fullStr	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
title_full_unstemmed	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
title_sort	nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/56e858fbf541481aa828e4e37d9df50a
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A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis

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