Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)

Frank C Barone,1 Cezary Marcinkiewicz,2,3 Jie Li,1 Yi Feng,4 Mark Sternberg,2 Peter I Lelkes,3 David Rosenbaum-Halevi,5 Jonathan A Gerstenhaber,3 Giora Z Feuerstein2 1SUNY Downstate Medical Center, Department of Neurology, Brooklyn, NY, USA; 2Debina Diagnostic Inc., Newtown Square, PA, USA; 3Depart...

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Autores principales:	Barone FC, Marcinkiewicz C, Li J, Feng Y, Sternberg M, Lelkes PI, Rosenbaum-Halevi D, Gerstenhaber JA, Feuerstein GZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Fluorescent Diamond Particles Carbon Nanoparticles Bio-compatibility Near Infra-Red imaging Particles Bio-Distribution Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/56ed253a29c64713bd47fd61a5c80dea
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spelling	oai:doaj.org-article:56ed253a29c64713bd47fd61a5c80dea2021-12-02T07:45:58ZLong-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)1178-2013https://doaj.org/article/56ed253a29c64713bd47fd61a5c80dea2019-02-01T00:00:00Zhttps://www.dovepress.com/long-term-biocompatibility-of-fluorescent-diamonds-nv-z800-nm-in-rats--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Frank C Barone,1 Cezary Marcinkiewicz,2,3 Jie Li,1 Yi Feng,4 Mark Sternberg,2 Peter I Lelkes,3 David Rosenbaum-Halevi,5 Jonathan A Gerstenhaber,3 Giora Z Feuerstein2 1SUNY Downstate Medical Center, Department of Neurology, Brooklyn, NY, USA; 2Debina Diagnostic Inc., Newtown Square, PA, USA; 3Department of Bioengineering, Temple University, College of Engineering, Philadelphia, PA, USA; 4WuXi AppTec (Suzhou) Co., Ltd., China; 5Texas University Health Center, TMC, Houston, TX, USA Background: Thromboembolic events are a major cause of heart attacks and strokes. However, diagnosis of the location of high risk vascular clots is hampered by lack of proper technologies for their detection .We recently reported on bio-engineered fluorescent diamond-(NV)-Z~800nm (FNDP-(NV)) conjugated with bitistatin (Bit) and proven its ability to identify iatrogenic blood clots in the rat carotid artery in vivo by Near Infra-Red (NIR) monitored by In Vivo Imaging System (IVIS). Purpose: The objective of the present research was to assess the in vivo biocompatibility of FNDP-(NV)-Z~800nm infused intravenously to rats. Multiple biological variables were assessed along this 12 week study commissioned in anticipation of regulatory requirements for a long-term safety assessment. Methods: Rats were infused under anesthesia with aforementioned dose of the FNDP-(NV), while equal number of animals served as control (vehicle treated). Over the 12 week observation period rats were tested for thriving, motor, sensory and cognitive functions. At the termination of study, blood samples were obtained under anesthesia for comprehensive hematology and biochemical assays. Furthermore, 6 whole organs (liver, spleen, brain, heart, lung and kidney) were collected and examined ex vivo for FNDP-NV) via NIR monitored by IVIS and histochemical inspection. Results: All animals survived, thrived (no change in body and organ growth). Neuro-behavioral functions remain intact. Hematology and biochemistry (including liver and kidney functions) were normal. Preferential FNDP-(NV) distribution identified the liver as the main long- term repository. Certified pathology reports indicated no outstanding of finding in all organs. Conclusion: The present study suggests outstanding biocompatibility of FNDP-(NV)-Z~800nm after long-term exposure in the rat. Keywords: nanocarbon particles, biocompatibility, liver toxicology, ex vivo IR organ imagingBarone FCMarcinkiewicz CLi JFeng YSternberg MLelkes PIRosenbaum-Halevi DGerstenhaber JAFeuerstein GZDove Medical PressarticleFluorescent Diamond ParticlesCarbon NanoparticlesBio-compatibilityNear Infra-Red imagingParticles Bio-DistributionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1163-1175 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fluorescent Diamond Particles Carbon Nanoparticles Bio-compatibility Near Infra-Red imaging Particles Bio-Distribution Medicine (General) R5-920
spellingShingle	Fluorescent Diamond Particles Carbon Nanoparticles Bio-compatibility Near Infra-Red imaging Particles Bio-Distribution Medicine (General) R5-920 Barone FC Marcinkiewicz C Li J Feng Y Sternberg M Lelkes PI Rosenbaum-Halevi D Gerstenhaber JA Feuerstein GZ Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
description	Frank C Barone,1 Cezary Marcinkiewicz,2,3 Jie Li,1 Yi Feng,4 Mark Sternberg,2 Peter I Lelkes,3 David Rosenbaum-Halevi,5 Jonathan A Gerstenhaber,3 Giora Z Feuerstein2 1SUNY Downstate Medical Center, Department of Neurology, Brooklyn, NY, USA; 2Debina Diagnostic Inc., Newtown Square, PA, USA; 3Department of Bioengineering, Temple University, College of Engineering, Philadelphia, PA, USA; 4WuXi AppTec (Suzhou) Co., Ltd., China; 5Texas University Health Center, TMC, Houston, TX, USA Background: Thromboembolic events are a major cause of heart attacks and strokes. However, diagnosis of the location of high risk vascular clots is hampered by lack of proper technologies for their detection .We recently reported on bio-engineered fluorescent diamond-(NV)-Z~800nm (FNDP-(NV)) conjugated with bitistatin (Bit) and proven its ability to identify iatrogenic blood clots in the rat carotid artery in vivo by Near Infra-Red (NIR) monitored by In Vivo Imaging System (IVIS). Purpose: The objective of the present research was to assess the in vivo biocompatibility of FNDP-(NV)-Z~800nm infused intravenously to rats. Multiple biological variables were assessed along this 12 week study commissioned in anticipation of regulatory requirements for a long-term safety assessment. Methods: Rats were infused under anesthesia with aforementioned dose of the FNDP-(NV), while equal number of animals served as control (vehicle treated). Over the 12 week observation period rats were tested for thriving, motor, sensory and cognitive functions. At the termination of study, blood samples were obtained under anesthesia for comprehensive hematology and biochemical assays. Furthermore, 6 whole organs (liver, spleen, brain, heart, lung and kidney) were collected and examined ex vivo for FNDP-NV) via NIR monitored by IVIS and histochemical inspection. Results: All animals survived, thrived (no change in body and organ growth). Neuro-behavioral functions remain intact. Hematology and biochemistry (including liver and kidney functions) were normal. Preferential FNDP-(NV) distribution identified the liver as the main long- term repository. Certified pathology reports indicated no outstanding of finding in all organs. Conclusion: The present study suggests outstanding biocompatibility of FNDP-(NV)-Z~800nm after long-term exposure in the rat. Keywords: nanocarbon particles, biocompatibility, liver toxicology, ex vivo IR organ imaging
format	article
author	Barone FC Marcinkiewicz C Li J Feng Y Sternberg M Lelkes PI Rosenbaum-Halevi D Gerstenhaber JA Feuerstein GZ
author_facet	Barone FC Marcinkiewicz C Li J Feng Y Sternberg M Lelkes PI Rosenbaum-Halevi D Gerstenhaber JA Feuerstein GZ
author_sort	Barone FC
title	Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
title_short	Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
title_full	Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
title_fullStr	Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
title_full_unstemmed	Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)
title_sort	long-term biocompatibility of fluorescent diamonds-(nv)-z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part iv)
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/56ed253a29c64713bd47fd61a5c80dea
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Long-term biocompatibility of fluorescent diamonds-(NV)-Z~800 nm in rats: survival, morbidity, histopathology, and particle distribution and excretion studies (part IV)

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