Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

Masahiro Yamada*, Takeshi Ueno*, Naoki Tsukimura, Takayuki Ikeda, Kaori Nakagawa, Norio Hori, Takeo Suzuki, Takahiro OgawaLaboratory of Bone and Implant Sciences, The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA Sch...

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Autores principales:	Suzuki T, Hori N, Nakagawa K, Ikeda T, Tsukimura N, Ueno T, Yamada M, Ogawa T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/56f15cbaa70745e98138eb11efc97d94
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spelling	oai:doaj.org-article:56f15cbaa70745e98138eb11efc97d942021-12-02T00:12:16ZBone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants1176-91141178-2013https://doaj.org/article/56f15cbaa70745e98138eb11efc97d942012-02-01T00:00:00Zhttp://www.dovepress.com/bone-integration-capability-of-nanopolymorphic-crystalline-hydroxyapat-a9295https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Masahiro Yamada, Takeshi Ueno, Naoki Tsukimura, Takayuki Ikeda, Kaori Nakagawa, Norio Hori, Takeo Suzuki, Takahiro OgawaLaboratory of Bone and Implant Sciences, The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, USA *These authors contributed equally to this workAbstract: The mechanism by which hydroxyapatite (HA)-coated titanium promotes bone&ndash;implant integration is largely unknown. Furthermore, refining the fabrication of nanostructured HA to the level applicable to the mass production process for titanium implants is challenging. This study reports successful creation of nanopolymorphic crystalline HA on microroughened titanium surfaces using a combination of flame spray and low-temperature calcination and tests its biological capability to enhance bone&ndash;implant integration. Sandblasted microroughened titanium implants and sandblasted + HA-coated titanium implants were subjected to biomechanical and histomorphometric analyses in a rat model. The HA was 55% crystallized and consisted of nanoscale needle-like architectures developed in various diameters, lengths, and orientations, which resulted in a 70% increase in surface area compared to noncoated microroughened surfaces. The HA was free from impurity contaminants, with a calcium/phosphorus ratio of 1.66 being equivalent to that of stoichiometric HA. As compared to microroughened implants, HA-coated implants increased the strength of bone&ndash;implant integration consistently at both early and late stages of healing. HA-coated implants showed an increased percentage of bone&ndash;implant contact and bone volume within 50 &micro;m proximity of the implant surface, as well as a remarkably reduced percentage of soft tissue intervention between bone and the implant surface. In contrast, bone volume outside the 50 &micro;m border was lower around HA-coated implants. Thus, this study demonstrated that the addition of pure nanopolymorphic crystalline HA to microroughened titanium not only accelerates but also enhances the level of bone&ndash;implant integration and identified the specific tissue morphogenesis parameters modulated by HA coating. In particular, the nanocrystalline HA was proven to be drastic in increasing osteoconductivity and inhibiting soft tissue infiltration, but the effect was limited to the immediate microenvironment surrounding the implant.Keywords: osseointegration, dental and orthopedic implant, nanotechnology, bone&ndash;implant integration, HA, calcium phosphateSuzuki THori NNakagawa KIkeda TTsukimura NUeno TYamada MOgawa TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 859-873 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Suzuki T Hori N Nakagawa K Ikeda T Tsukimura N Ueno T Yamada M Ogawa T Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
description	Masahiro Yamada, Takeshi Ueno, Naoki Tsukimura, Takayuki Ikeda, Kaori Nakagawa, Norio Hori, Takeo Suzuki, Takahiro OgawaLaboratory of Bone and Implant Sciences, The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, USA *These authors contributed equally to this workAbstract: The mechanism by which hydroxyapatite (HA)-coated titanium promotes bone&ndash;implant integration is largely unknown. Furthermore, refining the fabrication of nanostructured HA to the level applicable to the mass production process for titanium implants is challenging. This study reports successful creation of nanopolymorphic crystalline HA on microroughened titanium surfaces using a combination of flame spray and low-temperature calcination and tests its biological capability to enhance bone&ndash;implant integration. Sandblasted microroughened titanium implants and sandblasted + HA-coated titanium implants were subjected to biomechanical and histomorphometric analyses in a rat model. The HA was 55% crystallized and consisted of nanoscale needle-like architectures developed in various diameters, lengths, and orientations, which resulted in a 70% increase in surface area compared to noncoated microroughened surfaces. The HA was free from impurity contaminants, with a calcium/phosphorus ratio of 1.66 being equivalent to that of stoichiometric HA. As compared to microroughened implants, HA-coated implants increased the strength of bone&ndash;implant integration consistently at both early and late stages of healing. HA-coated implants showed an increased percentage of bone&ndash;implant contact and bone volume within 50 &micro;m proximity of the implant surface, as well as a remarkably reduced percentage of soft tissue intervention between bone and the implant surface. In contrast, bone volume outside the 50 &micro;m border was lower around HA-coated implants. Thus, this study demonstrated that the addition of pure nanopolymorphic crystalline HA to microroughened titanium not only accelerates but also enhances the level of bone&ndash;implant integration and identified the specific tissue morphogenesis parameters modulated by HA coating. In particular, the nanocrystalline HA was proven to be drastic in increasing osteoconductivity and inhibiting soft tissue infiltration, but the effect was limited to the immediate microenvironment surrounding the implant.Keywords: osseointegration, dental and orthopedic implant, nanotechnology, bone&ndash;implant integration, HA, calcium phosphate
format	article
author	Suzuki T Hori N Nakagawa K Ikeda T Tsukimura N Ueno T Yamada M Ogawa T
author_facet	Suzuki T Hori N Nakagawa K Ikeda T Tsukimura N Ueno T Yamada M Ogawa T
author_sort	Suzuki T
title	Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
title_short	Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
title_full	Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
title_fullStr	Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
title_full_unstemmed	Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
title_sort	bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/56f15cbaa70745e98138eb11efc97d94
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_version_	1718403866686390272

Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

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