BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation

Objectives. Some DNA viruses, such as BKPyV, are capable of inducing neoplastic transformation in human tissues through still unclear mechanisms. The goal of this study is to investigate the carcinogenic potential of BK polyomavirus (BKPyV) in human embryonic kidney 293 (Hek293) cells, dissecting th...

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Autores principales:	Sara Baldelli, Dolores Limongi, Cristiana Coni, Fabio Ciccarone, Marco Ciotti, Paola Checconi, Anna Teresa Palamara, Maria Rosa Ciriolo
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/56f2289e96dc45a3b073ce54552f9df8
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id	oai:doaj.org-article:56f2289e96dc45a3b073ce54552f9df8
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spelling	oai:doaj.org-article:56f2289e96dc45a3b073ce54552f9df82021-11-29T00:55:33ZBK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation1942-099410.1155/2021/9176993https://doaj.org/article/56f2289e96dc45a3b073ce54552f9df82021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9176993https://doaj.org/toc/1942-0994Objectives. Some DNA viruses, such as BKPyV, are capable of inducing neoplastic transformation in human tissues through still unclear mechanisms. The goal of this study is to investigate the carcinogenic potential of BK polyomavirus (BKPyV) in human embryonic kidney 293 (Hek293) cells, dissecting the molecular mechanism that determines the neoplastic transformation. Materials and Methods. BKPyV, isolated from urine samples of infected patients, was used to infect monolayers of Hek293 cells. Subsequently, intracellular redox changes, GSH/GSSH concentration by HPLC, and reactive oxygen/nitrogen species (ROS/RNS) production were monitored. Moreover, to understand the signaling pathway underlying the neoplastic transformation, the redox-sensitive HFS1-Hsp27 molecular axis was examined using the flavonoid quercetin and polishort hairpin RNA technologies. Results. The data obtained show that while BKPyV replication is closely linked to the transcription factor p53, the increase in Hek293 cell proliferation is due to the activation of the signaling pathway mediated by HSF1-Hsp27. In fact, its inhibition blocks viral replication and cell growth, respectively. Conclusions. The HSF1-Hsp27 signaling pathway is involved in BKPyV infection and cellular replication and its activation, which could be involved in cell transformation.Sara BaldelliDolores LimongiCristiana ConiFabio CiccaroneMarco CiottiPaola ChecconiAnna Teresa PalamaraMaria Rosa CirioloHindawi LimitedarticleCytologyQH573-671ENOxidative Medicine and Cellular Longevity, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Sara Baldelli Dolores Limongi Cristiana Coni Fabio Ciccarone Marco Ciotti Paola Checconi Anna Teresa Palamara Maria Rosa Ciriolo BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
description	Objectives. Some DNA viruses, such as BKPyV, are capable of inducing neoplastic transformation in human tissues through still unclear mechanisms. The goal of this study is to investigate the carcinogenic potential of BK polyomavirus (BKPyV) in human embryonic kidney 293 (Hek293) cells, dissecting the molecular mechanism that determines the neoplastic transformation. Materials and Methods. BKPyV, isolated from urine samples of infected patients, was used to infect monolayers of Hek293 cells. Subsequently, intracellular redox changes, GSH/GSSH concentration by HPLC, and reactive oxygen/nitrogen species (ROS/RNS) production were monitored. Moreover, to understand the signaling pathway underlying the neoplastic transformation, the redox-sensitive HFS1-Hsp27 molecular axis was examined using the flavonoid quercetin and polishort hairpin RNA technologies. Results. The data obtained show that while BKPyV replication is closely linked to the transcription factor p53, the increase in Hek293 cell proliferation is due to the activation of the signaling pathway mediated by HSF1-Hsp27. In fact, its inhibition blocks viral replication and cell growth, respectively. Conclusions. The HSF1-Hsp27 signaling pathway is involved in BKPyV infection and cellular replication and its activation, which could be involved in cell transformation.
format	article
author	Sara Baldelli Dolores Limongi Cristiana Coni Fabio Ciccarone Marco Ciotti Paola Checconi Anna Teresa Palamara Maria Rosa Ciriolo
author_facet	Sara Baldelli Dolores Limongi Cristiana Coni Fabio Ciccarone Marco Ciotti Paola Checconi Anna Teresa Palamara Maria Rosa Ciriolo
author_sort	Sara Baldelli
title	BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
title_short	BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
title_full	BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
title_fullStr	BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
title_full_unstemmed	BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation
title_sort	bk polyomavirus activates hsf1 stimulating human kidney hek293 cell proliferation
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/56f2289e96dc45a3b073ce54552f9df8
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_version_	1718407768960925696

BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation

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