Population imaging of synaptically released glutamate in mouse hippocampal slices

Summary: Glutamatergic neurotransmission is a widespread form of synaptic excitation in the mammalian brain. The development of genetically encoded fluorescent glutamate sensors allows monitoring synaptic signaling in living brain tissue in real time. Here, we describe single- and two-photon imaging...

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Autores principales:	Felix Unger, Arthur Konnerth, Benedikt Zott
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microscopy Model Organisms Neuroscience Molecular/Chemical Probes Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/56f50b6d49c7431eaa8ad434bc6d51c3
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spelling	oai:doaj.org-article:56f50b6d49c7431eaa8ad434bc6d51c32021-11-10T04:41:24ZPopulation imaging of synaptically released glutamate in mouse hippocampal slices2666-166710.1016/j.xpro.2021.100877https://doaj.org/article/56f50b6d49c7431eaa8ad434bc6d51c32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721005839https://doaj.org/toc/2666-1667Summary: Glutamatergic neurotransmission is a widespread form of synaptic excitation in the mammalian brain. The development of genetically encoded fluorescent glutamate sensors allows monitoring synaptic signaling in living brain tissue in real time. Here, we describe single- and two-photon imaging of synaptically evoked glutamatergic population signals in acute hippocampal slices expressing the fluorescent glutamate sensor SF-iGluSnFR.A184S in CA1 or CA3 pyramidal neurons. The protocol can be readily used to study defective synaptic glutamate signaling in mouse models of neuropsychiatric disorders, such as Alzheimer disease.For complete details on the use and execution of this protocol, please refer to Zott et al. (2019).Felix UngerArthur KonnerthBenedikt ZottElsevierarticleMicroscopyModel OrganismsNeuroscienceMolecular/Chemical ProbesScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100877- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microscopy Model Organisms Neuroscience Molecular/Chemical Probes Science (General) Q1-390
spellingShingle	Microscopy Model Organisms Neuroscience Molecular/Chemical Probes Science (General) Q1-390 Felix Unger Arthur Konnerth Benedikt Zott Population imaging of synaptically released glutamate in mouse hippocampal slices
description	Summary: Glutamatergic neurotransmission is a widespread form of synaptic excitation in the mammalian brain. The development of genetically encoded fluorescent glutamate sensors allows monitoring synaptic signaling in living brain tissue in real time. Here, we describe single- and two-photon imaging of synaptically evoked glutamatergic population signals in acute hippocampal slices expressing the fluorescent glutamate sensor SF-iGluSnFR.A184S in CA1 or CA3 pyramidal neurons. The protocol can be readily used to study defective synaptic glutamate signaling in mouse models of neuropsychiatric disorders, such as Alzheimer disease.For complete details on the use and execution of this protocol, please refer to Zott et al. (2019).
format	article
author	Felix Unger Arthur Konnerth Benedikt Zott
author_facet	Felix Unger Arthur Konnerth Benedikt Zott
author_sort	Felix Unger
title	Population imaging of synaptically released glutamate in mouse hippocampal slices
title_short	Population imaging of synaptically released glutamate in mouse hippocampal slices
title_full	Population imaging of synaptically released glutamate in mouse hippocampal slices
title_fullStr	Population imaging of synaptically released glutamate in mouse hippocampal slices
title_full_unstemmed	Population imaging of synaptically released glutamate in mouse hippocampal slices
title_sort	population imaging of synaptically released glutamate in mouse hippocampal slices
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/56f50b6d49c7431eaa8ad434bc6d51c3
work_keys_str_mv	AT felixunger populationimagingofsynapticallyreleasedglutamateinmousehippocampalslices AT arthurkonnerth populationimagingofsynapticallyreleasedglutamateinmousehippocampalslices AT benediktzott populationimagingofsynapticallyreleasedglutamateinmousehippocampalslices
_version_	1718440557164888064

Population imaging of synaptically released glutamate in mouse hippocampal slices

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