Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.

The calcium ion (Ca(2+)) is an important messenger for signal transduction, and the intracellular Ca(2+) concentration ([Ca(2+)](i)) changes in response to an excitation of the cell. To reveal the spatiotemporal properties of the propagation of an excitatory signal with action potentials in the prim...

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Autores principales:	Makoto Osanai, Satoshi Tanaka, Yusuke Takeno, Shouta Takimoto, Tetsuya Yagi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56f5b18dc73541e29b783d3af5f6d0d4
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spelling	oai:doaj.org-article:56f5b18dc73541e29b783d3af5f6d0d42021-11-18T07:02:40ZSpatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.1932-620310.1371/journal.pone.0013738https://doaj.org/article/56f5b18dc73541e29b783d3af5f6d0d42010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21060776/?tool=EBIhttps://doaj.org/toc/1932-6203The calcium ion (Ca(2+)) is an important messenger for signal transduction, and the intracellular Ca(2+) concentration ([Ca(2+)](i)) changes in response to an excitation of the cell. To reveal the spatiotemporal properties of the propagation of an excitatory signal with action potentials in the primary visual cortical circuit, we conducted a Ca(2+) imaging study on slices of the mouse visual cortex. Electrical stimulation of layer 4 evoked [Ca(2+)](i) transients around the stimulus electrode. Subsequently, the high [Ca(2+)](i) region mainly propagated perpendicular to the cortical layer (vertical propagation), with horizontal propagation being restricted. When the excitatory synaptic transmission was blocked, only weak and concentric [Ca(2+)](i) transients were observed. When the action potential was blocked, the [Ca(2+)](i) transients disappeared almost completely. These results suggested that the action potential contributed to the induction of the [Ca(2+)](i) transients, and that excitatory synaptic connections were involved in the propagation of the high [Ca(2+)](i) region in the primary visual cortical circuit. To elucidate the involvement of inhibitory synaptic connections in signal propagation in the primary visual cortex, the GABA(A) receptor inhibitor bicuculline was applied. In this case, the evoked signal propagated from layer 4 to the entire field of view, and the prolonged [Ca(2+)](i) transients were observed compared with the control condition. Our results suggest that excitatory neurons are widely connected to each other over the entire primary visual cortex with recurrent synapses, and inhibitory neurons play a fundamental role in the organization of functional sub-networks by restricting the propagation of excitation signals.Makoto OsanaiSatoshi TanakaYusuke TakenoShouta TakimotoTetsuya YagiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13738 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Makoto Osanai Satoshi Tanaka Yusuke Takeno Shouta Takimoto Tetsuya Yagi Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
description	The calcium ion (Ca(2+)) is an important messenger for signal transduction, and the intracellular Ca(2+) concentration ([Ca(2+)](i)) changes in response to an excitation of the cell. To reveal the spatiotemporal properties of the propagation of an excitatory signal with action potentials in the primary visual cortical circuit, we conducted a Ca(2+) imaging study on slices of the mouse visual cortex. Electrical stimulation of layer 4 evoked [Ca(2+)](i) transients around the stimulus electrode. Subsequently, the high [Ca(2+)](i) region mainly propagated perpendicular to the cortical layer (vertical propagation), with horizontal propagation being restricted. When the excitatory synaptic transmission was blocked, only weak and concentric [Ca(2+)](i) transients were observed. When the action potential was blocked, the [Ca(2+)](i) transients disappeared almost completely. These results suggested that the action potential contributed to the induction of the [Ca(2+)](i) transients, and that excitatory synaptic connections were involved in the propagation of the high [Ca(2+)](i) region in the primary visual cortical circuit. To elucidate the involvement of inhibitory synaptic connections in signal propagation in the primary visual cortex, the GABA(A) receptor inhibitor bicuculline was applied. In this case, the evoked signal propagated from layer 4 to the entire field of view, and the prolonged [Ca(2+)](i) transients were observed compared with the control condition. Our results suggest that excitatory neurons are widely connected to each other over the entire primary visual cortex with recurrent synapses, and inhibitory neurons play a fundamental role in the organization of functional sub-networks by restricting the propagation of excitation signals.
format	article
author	Makoto Osanai Satoshi Tanaka Yusuke Takeno Shouta Takimoto Tetsuya Yagi
author_facet	Makoto Osanai Satoshi Tanaka Yusuke Takeno Shouta Takimoto Tetsuya Yagi
author_sort	Makoto Osanai
title	Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
title_short	Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
title_full	Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
title_fullStr	Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
title_full_unstemmed	Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
title_sort	spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/56f5b18dc73541e29b783d3af5f6d0d4
work_keys_str_mv	AT makotoosanai spatiotemporalpropertiesoftheactionpotentialpropagationinthemousevisualcorticalsliceanalyzedbycalciumimaging AT satoshitanaka spatiotemporalpropertiesoftheactionpotentialpropagationinthemousevisualcorticalsliceanalyzedbycalciumimaging AT yusuketakeno spatiotemporalpropertiesoftheactionpotentialpropagationinthemousevisualcorticalsliceanalyzedbycalciumimaging AT shoutatakimoto spatiotemporalpropertiesoftheactionpotentialpropagationinthemousevisualcorticalsliceanalyzedbycalciumimaging AT tetsuyayagi spatiotemporalpropertiesoftheactionpotentialpropagationinthemousevisualcorticalsliceanalyzedbycalciumimaging
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Spatiotemporal properties of the action potential propagation in the mouse visual cortical slice analyzed by calcium imaging.

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