AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.

The main functions of spermatozoa required for fertilization are dependent on the energy status and metabolism. AMP-activated kinase, AMPK, acts a sensor and regulator of cell metabolism. As AMPK studies have been focused on somatic cells, our aim was to investigate the expression of AMPK protein in...

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Autores principales:	Ana Hurtado de Llera, David Martin-Hidalgo, María C Gil, Luis J Garcia-Marin, María J Bragado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5700c31b657442548d14cb3221ae6bae
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spelling	oai:doaj.org-article:5700c31b657442548d14cb3221ae6bae2021-11-18T07:15:30ZAMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.1932-620310.1371/journal.pone.0038840https://doaj.org/article/5700c31b657442548d14cb3221ae6bae2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22719961/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The main functions of spermatozoa required for fertilization are dependent on the energy status and metabolism. AMP-activated kinase, AMPK, acts a sensor and regulator of cell metabolism. As AMPK studies have been focused on somatic cells, our aim was to investigate the expression of AMPK protein in spermatozoa and its possible role in regulating motility. Spermatozoa from boar ejaculates were isolated and incubated under different conditions (38,5°C or 17°C, basal medium TBM or medium with Ca(2+) and bicarbonate TCM, time from 1-24 hours) in presence or absence of AMPK inhibitor, compound C (CC, 30 µM). Western blotting reveals that AMPK is expressed in boar spermatozoa at relatively higher levels than in somatic cells. AMPK phosphorylation (activation) in spermatozoa is temperature-dependent, as it is undetectable at semen preservation temperature (17°C) and increases at 38,5°C in a time-dependent manner. AMPK phosphorylation is independent of the presence of Ca(2+) and/or bicarbonate in the medium. We confirm that CC effectively blocks AMPK phosphorylation in boar spermatozoa. Analysis of spermatozoa motility by CASA shows that CC treatment either in TBM or in TCM causes a significant reduction of any spermatozoa motility parameter in a time-dependent manner. Thus, AMPK inhibition significantly decreases the percentages of motile and rapid spermatozoa, significantly reduces spermatozoa velocities VAP, VCL and affects other motility parameters and coefficients. CC treatment does not cause additional side effects in spermatozoa that might lead to a lower viability even at 24 h incubation. Our results show that AMPK is expressed in spermatozoa at high levels and is phosphorylated under physiological conditions. Moreover, our study suggests that AMPK regulates a relevant function of spermatozoa, motility, which is essential for their ultimate role of fertilization.Ana Hurtado de LleraDavid Martin-HidalgoMaría C GilLuis J Garcia-MarinMaría J BragadoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38840 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Hurtado de Llera David Martin-Hidalgo María C Gil Luis J Garcia-Marin María J Bragado AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
description	The main functions of spermatozoa required for fertilization are dependent on the energy status and metabolism. AMP-activated kinase, AMPK, acts a sensor and regulator of cell metabolism. As AMPK studies have been focused on somatic cells, our aim was to investigate the expression of AMPK protein in spermatozoa and its possible role in regulating motility. Spermatozoa from boar ejaculates were isolated and incubated under different conditions (38,5°C or 17°C, basal medium TBM or medium with Ca(2+) and bicarbonate TCM, time from 1-24 hours) in presence or absence of AMPK inhibitor, compound C (CC, 30 µM). Western blotting reveals that AMPK is expressed in boar spermatozoa at relatively higher levels than in somatic cells. AMPK phosphorylation (activation) in spermatozoa is temperature-dependent, as it is undetectable at semen preservation temperature (17°C) and increases at 38,5°C in a time-dependent manner. AMPK phosphorylation is independent of the presence of Ca(2+) and/or bicarbonate in the medium. We confirm that CC effectively blocks AMPK phosphorylation in boar spermatozoa. Analysis of spermatozoa motility by CASA shows that CC treatment either in TBM or in TCM causes a significant reduction of any spermatozoa motility parameter in a time-dependent manner. Thus, AMPK inhibition significantly decreases the percentages of motile and rapid spermatozoa, significantly reduces spermatozoa velocities VAP, VCL and affects other motility parameters and coefficients. CC treatment does not cause additional side effects in spermatozoa that might lead to a lower viability even at 24 h incubation. Our results show that AMPK is expressed in spermatozoa at high levels and is phosphorylated under physiological conditions. Moreover, our study suggests that AMPK regulates a relevant function of spermatozoa, motility, which is essential for their ultimate role of fertilization.
format	article
author	Ana Hurtado de Llera David Martin-Hidalgo María C Gil Luis J Garcia-Marin María J Bragado
author_facet	Ana Hurtado de Llera David Martin-Hidalgo María C Gil Luis J Garcia-Marin María J Bragado
author_sort	Ana Hurtado de Llera
title	AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
title_short	AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
title_full	AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
title_fullStr	AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
title_full_unstemmed	AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.
title_sort	amp-activated kinase ampk is expressed in boar spermatozoa and regulates motility.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5700c31b657442548d14cb3221ae6bae
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_version_	1718423725815103488

AMP-activated kinase AMPK is expressed in boar spermatozoa and regulates motility.

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