Spatio-Temporal Profiling of Metarhizium anisopliae—Responsive microRNAs Involved in Modulation of Plutella xylostella Immunity and Development

Metarhizium anisopliae, a ubiquitous pathogenic fungus, regulates a wide array of the insect pest population. The fungus has been employed to control Plutella xylostella, an insecticide-resistant destructive lepidopteran pest, which causes substantial economic l...

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Autores principales:	Junaid Zafar, Yuxin Zhang, Junlin Huang, Shoaib Freed, Rana Fartab Shoukat, Xiaoxia Xu, Fengliang Jin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biocontrol midgut small RNA gene silencing diamond back moth Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5703de44d8924f318294109d3e67fc33
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spelling	oai:doaj.org-article:5703de44d8924f318294109d3e67fc332021-11-25T18:06:00ZSpatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development10.3390/jof71109422309-608Xhttps://doaj.org/article/5703de44d8924f318294109d3e67fc332021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/942https://doaj.org/toc/2309-608X<i>Metarhizium anisopliae</i>, a ubiquitous pathogenic fungus, regulates a wide array of the insect pest population. The fungus has been employed to control <i>Plutella xylostella</i>, an insecticide-resistant destructive lepidopteran pest, which causes substantial economic losses in crops worldwide. Integration of modern gene-silencing technologies in pest control strategies has become more crucial to counter pesticide-resistant insects. MicroRNAs (miRNA) play essential roles in the various biological process via post-transcriptional gene regulation. In the present study, RNA-seq analysis of control (CK36h, CK72h) and fungal-infected (T36h, T72h) midguts was performed to reveal underlying molecular mechanisms occurring in larval midgut at different time courses. We aimed at exploring <i>M. anisopliae</i>-responsive miRNAs and their target genes involved in development and immunity. After data filtration, a combined set of 170 miRNAs were identified from all libraries. Interestingly, miR-281, miR-263, miR-1, miR-6094 and miR-8 were listed among the most abundantly expressed conserved miRNAs. Furthermore, we experimentally studied the role of differentially expressed miR-11912-5p in regulating corresponding target trypsin-like serine proteinase (<i>Px_TLSP</i>). The luciferase assay (in vitro) revealed that miRNA-11912-5p significantly downregulated its target gene, suggesting it might play a crucial role in defense mechanism of <i>P. xylostella</i> against <i>M.+ anisopliae</i> infection. We used synthetic miRNA mimic/inhibitor (in vivo), to overexpress/silence miRNA, which showed harmful effects on larval duration, survival and adult fecundity. Additionally, fungal application in the presence of mimics revealed enhanced sensitivity of <i>P. xylostella</i> to infection. Our finding provides an insight into the relatively obscure molecular mechanisms involved in insect midgut during the fungal infection.Junaid ZafarYuxin ZhangJunlin HuangShoaib FreedRana Fartab ShoukatXiaoxia XuFengliang JinMDPI AGarticlebiocontrolmidgutsmall RNAgene silencingdiamond back mothBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 942, p 942 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocontrol midgut small RNA gene silencing diamond back moth Biology (General) QH301-705.5
spellingShingle	biocontrol midgut small RNA gene silencing diamond back moth Biology (General) QH301-705.5 Junaid Zafar Yuxin Zhang Junlin Huang Shoaib Freed Rana Fartab Shoukat Xiaoxia Xu Fengliang Jin Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
description	<i>Metarhizium anisopliae</i>, a ubiquitous pathogenic fungus, regulates a wide array of the insect pest population. The fungus has been employed to control <i>Plutella xylostella</i>, an insecticide-resistant destructive lepidopteran pest, which causes substantial economic losses in crops worldwide. Integration of modern gene-silencing technologies in pest control strategies has become more crucial to counter pesticide-resistant insects. MicroRNAs (miRNA) play essential roles in the various biological process via post-transcriptional gene regulation. In the present study, RNA-seq analysis of control (CK36h, CK72h) and fungal-infected (T36h, T72h) midguts was performed to reveal underlying molecular mechanisms occurring in larval midgut at different time courses. We aimed at exploring <i>M. anisopliae</i>-responsive miRNAs and their target genes involved in development and immunity. After data filtration, a combined set of 170 miRNAs were identified from all libraries. Interestingly, miR-281, miR-263, miR-1, miR-6094 and miR-8 were listed among the most abundantly expressed conserved miRNAs. Furthermore, we experimentally studied the role of differentially expressed miR-11912-5p in regulating corresponding target trypsin-like serine proteinase (<i>Px_TLSP</i>). The luciferase assay (in vitro) revealed that miRNA-11912-5p significantly downregulated its target gene, suggesting it might play a crucial role in defense mechanism of <i>P. xylostella</i> against <i>M.+ anisopliae</i> infection. We used synthetic miRNA mimic/inhibitor (in vivo), to overexpress/silence miRNA, which showed harmful effects on larval duration, survival and adult fecundity. Additionally, fungal application in the presence of mimics revealed enhanced sensitivity of <i>P. xylostella</i> to infection. Our finding provides an insight into the relatively obscure molecular mechanisms involved in insect midgut during the fungal infection.
format	article
author	Junaid Zafar Yuxin Zhang Junlin Huang Shoaib Freed Rana Fartab Shoukat Xiaoxia Xu Fengliang Jin
author_facet	Junaid Zafar Yuxin Zhang Junlin Huang Shoaib Freed Rana Fartab Shoukat Xiaoxia Xu Fengliang Jin
author_sort	Junaid Zafar
title	Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
title_short	Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
title_full	Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
title_fullStr	Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
title_full_unstemmed	Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development
title_sort	spatio-temporal profiling of <i>metarhizium anisopliae</i>—responsive micrornas involved in modulation of <i>plutella xylostella</i> immunity and development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5703de44d8924f318294109d3e67fc33
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Spatio-Temporal Profiling of <i>Metarhizium anisopliae</i>—Responsive microRNAs Involved in Modulation of <i>Plutella xylostella</i> Immunity and Development

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