Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran

Abstract The purpose of this study was to determine the mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from biopsy samples that were collected from the endoscopic ward of Shahrekord Hajar teaching Hospital and also to study the frequency of virulence fact...

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Autores principales:	Razieh Sadat Hosseini, Ghorbanali Rahimian, Mohammad Hadi Shafigh, Majid Validi, Mansoor Khaledi, Abolfazl Gholipour
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Helicobacter pylori Antibiotic resistance Real-time PCR Clarithromycin Virulence factors Biotechnology TP248.13-248.65 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5703f4e79aaa4187a2fad549f1f4929d
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spelling	oai:doaj.org-article:5703f4e79aaa4187a2fad549f1f4929d2021-11-07T12:21:57ZCorrelation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran10.1186/s13568-021-01310-92191-0855https://doaj.org/article/5703f4e79aaa4187a2fad549f1f4929d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13568-021-01310-9https://doaj.org/toc/2191-0855Abstract The purpose of this study was to determine the mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from biopsy samples that were collected from the endoscopic ward of Shahrekord Hajar teaching Hospital and also to study the frequency of virulence factor and their correlation and pathological findings with clarithromycin resistance during the years 2019–2020. In this cross-sectional descriptive study, 152 patients with Helicobacter pylori infection were considered, and then, two common A2142G and A2143G mutations in the 23SrRNA gene associated with resistance were analyzed by Real-time PCR (Taq man). The presence of vacA, iceA1, iceA2, cagA, babA2, and oipA virulence genes was investigated by PCR and electrophoresis in 8% polyacrylamide gel. Then, data were analyzed using the relevant statistical tests. In this study, the frequency of Helicobacter pylori was 76% and the frequency of mutant isolates was 57.2%. The frequencies of A2142G and A2143G point mutations were 42.1% and 28.3%. There was a significant correlation among oipA, vacA, and iceA1 virulence factors, type of disease, chronic inflammatory score, and glandular atrophy with the antibiotic resistance to clarithromycin. There was no significant correlation between the age and sex of the patients with antibiotic resistance. According to the results of this study, it seems that the use of clarithromycin to combat this bacterium should be limited.Razieh Sadat HosseiniGhorbanali RahimianMohammad Hadi ShafighMajid ValidiMansoor KhalediAbolfazl GholipourSpringerOpenarticleHelicobacter pyloriAntibiotic resistanceReal-time PCRClarithromycinVirulence factorsBiotechnologyTP248.13-248.65MicrobiologyQR1-502ENAMB Express, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Helicobacter pylori Antibiotic resistance Real-time PCR Clarithromycin Virulence factors Biotechnology TP248.13-248.65 Microbiology QR1-502
spellingShingle	Helicobacter pylori Antibiotic resistance Real-time PCR Clarithromycin Virulence factors Biotechnology TP248.13-248.65 Microbiology QR1-502 Razieh Sadat Hosseini Ghorbanali Rahimian Mohammad Hadi Shafigh Majid Validi Mansoor Khaledi Abolfazl Gholipour Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
description	Abstract The purpose of this study was to determine the mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from biopsy samples that were collected from the endoscopic ward of Shahrekord Hajar teaching Hospital and also to study the frequency of virulence factor and their correlation and pathological findings with clarithromycin resistance during the years 2019–2020. In this cross-sectional descriptive study, 152 patients with Helicobacter pylori infection were considered, and then, two common A2142G and A2143G mutations in the 23SrRNA gene associated with resistance were analyzed by Real-time PCR (Taq man). The presence of vacA, iceA1, iceA2, cagA, babA2, and oipA virulence genes was investigated by PCR and electrophoresis in 8% polyacrylamide gel. Then, data were analyzed using the relevant statistical tests. In this study, the frequency of Helicobacter pylori was 76% and the frequency of mutant isolates was 57.2%. The frequencies of A2142G and A2143G point mutations were 42.1% and 28.3%. There was a significant correlation among oipA, vacA, and iceA1 virulence factors, type of disease, chronic inflammatory score, and glandular atrophy with the antibiotic resistance to clarithromycin. There was no significant correlation between the age and sex of the patients with antibiotic resistance. According to the results of this study, it seems that the use of clarithromycin to combat this bacterium should be limited.
format	article
author	Razieh Sadat Hosseini Ghorbanali Rahimian Mohammad Hadi Shafigh Majid Validi Mansoor Khaledi Abolfazl Gholipour
author_facet	Razieh Sadat Hosseini Ghorbanali Rahimian Mohammad Hadi Shafigh Majid Validi Mansoor Khaledi Abolfazl Gholipour
author_sort	Razieh Sadat Hosseini
title	Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
title_short	Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
title_full	Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
title_fullStr	Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
title_full_unstemmed	Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran
title_sort	correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in helicobacter pylori infected patients in shahrekord, southwest iran
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/5703f4e79aaa4187a2fad549f1f4929d
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Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran

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