An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.

Plant growth and development are acutely sensitive to high ambient temperature caused in part due to climate change. However, the mechanism of high ambient temperature signaling is not well defined. Here, we show that HECATEs (HEC1 and HEC2), two helix-loop-helix transcription factors, inhibit therm...

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Autores principales:	Sanghwa Lee, Ling Zhu, Enamul Huq
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5705272425734bb7af0327bb49a0bacc
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spelling	oai:doaj.org-article:5705272425734bb7af0327bb49a0bacc2021-12-02T20:02:43ZAn autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.1553-73901553-740410.1371/journal.pgen.1009595https://doaj.org/article/5705272425734bb7af0327bb49a0bacc2021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009595https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Plant growth and development are acutely sensitive to high ambient temperature caused in part due to climate change. However, the mechanism of high ambient temperature signaling is not well defined. Here, we show that HECATEs (HEC1 and HEC2), two helix-loop-helix transcription factors, inhibit thermomorphogenesis. While the expression of HEC1 and HEC2 is increased and HEC2 protein is stabilized at high ambient temperature, hec1hec2 double mutant showed exaggerated thermomorphogenesis. Analyses of the four PHYTOCHROME INTERACTING FACTOR (PIF1, PIF3, PIF4 and PIF5) mutants and overexpression lines showed that they all contribute to promote thermomorphogenesis. Furthermore, genetic analysis showed that pifQ is epistatic to hec1hec2. HECs and PIFs oppositely control the expression of many genes in response to high ambient temperature. PIFs activate the expression of HECs in response to high ambient temperature. HEC2 in turn interacts with PIF4 both in yeast and in vivo. In the absence of HECs, PIF4 binding to its own promoter as well as the target gene promoters was enhanced, indicating that HECs control PIF4 activity via heterodimerization. Overall, these data suggest that PIF4-HEC forms an autoregulatory composite negative feedback loop that controls growth genes to modulate thermomorphogenesis.Sanghwa LeeLing ZhuEnamul HuqPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 6, p e1009595 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Sanghwa Lee Ling Zhu Enamul Huq An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
description	Plant growth and development are acutely sensitive to high ambient temperature caused in part due to climate change. However, the mechanism of high ambient temperature signaling is not well defined. Here, we show that HECATEs (HEC1 and HEC2), two helix-loop-helix transcription factors, inhibit thermomorphogenesis. While the expression of HEC1 and HEC2 is increased and HEC2 protein is stabilized at high ambient temperature, hec1hec2 double mutant showed exaggerated thermomorphogenesis. Analyses of the four PHYTOCHROME INTERACTING FACTOR (PIF1, PIF3, PIF4 and PIF5) mutants and overexpression lines showed that they all contribute to promote thermomorphogenesis. Furthermore, genetic analysis showed that pifQ is epistatic to hec1hec2. HECs and PIFs oppositely control the expression of many genes in response to high ambient temperature. PIFs activate the expression of HECs in response to high ambient temperature. HEC2 in turn interacts with PIF4 both in yeast and in vivo. In the absence of HECs, PIF4 binding to its own promoter as well as the target gene promoters was enhanced, indicating that HECs control PIF4 activity via heterodimerization. Overall, these data suggest that PIF4-HEC forms an autoregulatory composite negative feedback loop that controls growth genes to modulate thermomorphogenesis.
format	article
author	Sanghwa Lee Ling Zhu Enamul Huq
author_facet	Sanghwa Lee Ling Zhu Enamul Huq
author_sort	Sanghwa Lee
title	An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
title_short	An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
title_full	An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
title_fullStr	An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
title_full_unstemmed	An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.
title_sort	autoregulatory negative feedback loop controls thermomorphogenesis in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5705272425734bb7af0327bb49a0bacc
work_keys_str_mv	AT sanghwalee anautoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis AT lingzhu anautoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis AT enamulhuq anautoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis AT sanghwalee autoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis AT lingzhu autoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis AT enamulhuq autoregulatorynegativefeedbackloopcontrolsthermomorphogenesisinarabidopsis
_version_	1718375660468043776

An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis.

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