Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.

Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to...

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Autores principales:	Dionysios V Chartoumpekis, Apostolos Zaravinos, Panos G Ziros, Ralitsa P Iskrenova, Agathoklis I Psyrogiannis, Venetsana E Kyriazopoulou, Ioannis G Habeos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/570c2d09f4ba4b4e994bc22bc6e327cd
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spelling	oai:doaj.org-article:570c2d09f4ba4b4e994bc22bc6e327cd2021-11-18T07:23:10ZDifferential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.1932-620310.1371/journal.pone.0034872https://doaj.org/article/570c2d09f4ba4b4e994bc22bc6e327cd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22496873/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to the identification of novel biomarkers and therapeutic targets for the development of anti-obesity drugs. MicroRNAs (miRNAs) have been shown to play regulatory roles in several biological processes. They have become a growing research field and consist of promising pharmaceutical targets in various fields such as cancer, metabolism, etc. The present study investigated the possible implication of miRNAs in adipose tissue during the development of obesity using as a model the C57BLJ6 mice fed a high-fat diet.C57BLJ6 wild type male mice were fed either a standard (SD) or a high-fat diet (HFD) for 5 months. Total RNA was prepared from white adipose tissue and was used for microRNA profiling and qPCR.Twenty-two of the most differentially expressed miRNAs, as identified by the microRNA profiling were validated using qPCR. The results of the present study confirmed previous results. The up-regulation of mmu-miR-222 and the down-regulation of mmu-miR-200b, mmu-miR-200c, mmu-miR-204, mmu-miR-30a, mmu-miR-193, mmu-miR-378 and mmu-miR-30e after HFD feeding has also been previously reported. On the other hand, we show for the first time the up-regulation of mmu-miR-342-3p, mmu-miR-142-3p, mmu-miR-142-5p, mmu-miR-21, mmu-miR-146a, mmu-miR-146b, mmu-miR-379 and the down-regulation of mmu-miR-122, mmu-miR-133b, mmu-miR-1, mmu-miR-30a*, mmu-miR-192 and mmu-miR-203 during the development of obesity. However, future studies are warranted in order to understand the exact role that miRNAs play in adipogenesis and obesity.Dionysios V ChartoumpekisApostolos ZaravinosPanos G ZirosRalitsa P IskrenovaAgathoklis I PsyrogiannisVenetsana E KyriazopoulouIoannis G HabeosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34872 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dionysios V Chartoumpekis Apostolos Zaravinos Panos G Ziros Ralitsa P Iskrenova Agathoklis I Psyrogiannis Venetsana E Kyriazopoulou Ioannis G Habeos Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
description	Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to the identification of novel biomarkers and therapeutic targets for the development of anti-obesity drugs. MicroRNAs (miRNAs) have been shown to play regulatory roles in several biological processes. They have become a growing research field and consist of promising pharmaceutical targets in various fields such as cancer, metabolism, etc. The present study investigated the possible implication of miRNAs in adipose tissue during the development of obesity using as a model the C57BLJ6 mice fed a high-fat diet.C57BLJ6 wild type male mice were fed either a standard (SD) or a high-fat diet (HFD) for 5 months. Total RNA was prepared from white adipose tissue and was used for microRNA profiling and qPCR.Twenty-two of the most differentially expressed miRNAs, as identified by the microRNA profiling were validated using qPCR. The results of the present study confirmed previous results. The up-regulation of mmu-miR-222 and the down-regulation of mmu-miR-200b, mmu-miR-200c, mmu-miR-204, mmu-miR-30a, mmu-miR-193, mmu-miR-378 and mmu-miR-30e after HFD feeding has also been previously reported. On the other hand, we show for the first time the up-regulation of mmu-miR-342-3p, mmu-miR-142-3p, mmu-miR-142-5p, mmu-miR-21, mmu-miR-146a, mmu-miR-146b, mmu-miR-379 and the down-regulation of mmu-miR-122, mmu-miR-133b, mmu-miR-1, mmu-miR-30a*, mmu-miR-192 and mmu-miR-203 during the development of obesity. However, future studies are warranted in order to understand the exact role that miRNAs play in adipogenesis and obesity.
format	article
author	Dionysios V Chartoumpekis Apostolos Zaravinos Panos G Ziros Ralitsa P Iskrenova Agathoklis I Psyrogiannis Venetsana E Kyriazopoulou Ioannis G Habeos
author_facet	Dionysios V Chartoumpekis Apostolos Zaravinos Panos G Ziros Ralitsa P Iskrenova Agathoklis I Psyrogiannis Venetsana E Kyriazopoulou Ioannis G Habeos
author_sort	Dionysios V Chartoumpekis
title	Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_short	Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_full	Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_fullStr	Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_full_unstemmed	Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_sort	differential expression of micrornas in adipose tissue after long-term high-fat diet-induced obesity in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/570c2d09f4ba4b4e994bc22bc6e327cd
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Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.

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