Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.

Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to...

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Autores principales: Dionysios V Chartoumpekis, Apostolos Zaravinos, Panos G Ziros, Ralitsa P Iskrenova, Agathoklis I Psyrogiannis, Venetsana E Kyriazopoulou, Ioannis G Habeos
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:570c2d09f4ba4b4e994bc22bc6e327cd2021-11-18T07:23:10ZDifferential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.1932-620310.1371/journal.pone.0034872https://doaj.org/article/570c2d09f4ba4b4e994bc22bc6e327cd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22496873/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to the identification of novel biomarkers and therapeutic targets for the development of anti-obesity drugs. MicroRNAs (miRNAs) have been shown to play regulatory roles in several biological processes. They have become a growing research field and consist of promising pharmaceutical targets in various fields such as cancer, metabolism, etc. The present study investigated the possible implication of miRNAs in adipose tissue during the development of obesity using as a model the C57BLJ6 mice fed a high-fat diet.C57BLJ6 wild type male mice were fed either a standard (SD) or a high-fat diet (HFD) for 5 months. Total RNA was prepared from white adipose tissue and was used for microRNA profiling and qPCR.Twenty-two of the most differentially expressed miRNAs, as identified by the microRNA profiling were validated using qPCR. The results of the present study confirmed previous results. The up-regulation of mmu-miR-222 and the down-regulation of mmu-miR-200b, mmu-miR-200c, mmu-miR-204, mmu-miR-30a*, mmu-miR-193, mmu-miR-378 and mmu-miR-30e* after HFD feeding has also been previously reported. On the other hand, we show for the first time the up-regulation of mmu-miR-342-3p, mmu-miR-142-3p, mmu-miR-142-5p, mmu-miR-21, mmu-miR-146a, mmu-miR-146b, mmu-miR-379 and the down-regulation of mmu-miR-122, mmu-miR-133b, mmu-miR-1, mmu-miR-30a*, mmu-miR-192 and mmu-miR-203 during the development of obesity. However, future studies are warranted in order to understand the exact role that miRNAs play in adipogenesis and obesity.Dionysios V ChartoumpekisApostolos ZaravinosPanos G ZirosRalitsa P IskrenovaAgathoklis I PsyrogiannisVenetsana E KyriazopoulouIoannis G HabeosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34872 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Dionysios V Chartoumpekis
Apostolos Zaravinos
Panos G Ziros
Ralitsa P Iskrenova
Agathoklis I Psyrogiannis
Venetsana E Kyriazopoulou
Ioannis G Habeos
Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
description Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to the identification of novel biomarkers and therapeutic targets for the development of anti-obesity drugs. MicroRNAs (miRNAs) have been shown to play regulatory roles in several biological processes. They have become a growing research field and consist of promising pharmaceutical targets in various fields such as cancer, metabolism, etc. The present study investigated the possible implication of miRNAs in adipose tissue during the development of obesity using as a model the C57BLJ6 mice fed a high-fat diet.C57BLJ6 wild type male mice were fed either a standard (SD) or a high-fat diet (HFD) for 5 months. Total RNA was prepared from white adipose tissue and was used for microRNA profiling and qPCR.Twenty-two of the most differentially expressed miRNAs, as identified by the microRNA profiling were validated using qPCR. The results of the present study confirmed previous results. The up-regulation of mmu-miR-222 and the down-regulation of mmu-miR-200b, mmu-miR-200c, mmu-miR-204, mmu-miR-30a*, mmu-miR-193, mmu-miR-378 and mmu-miR-30e* after HFD feeding has also been previously reported. On the other hand, we show for the first time the up-regulation of mmu-miR-342-3p, mmu-miR-142-3p, mmu-miR-142-5p, mmu-miR-21, mmu-miR-146a, mmu-miR-146b, mmu-miR-379 and the down-regulation of mmu-miR-122, mmu-miR-133b, mmu-miR-1, mmu-miR-30a*, mmu-miR-192 and mmu-miR-203 during the development of obesity. However, future studies are warranted in order to understand the exact role that miRNAs play in adipogenesis and obesity.
format article
author Dionysios V Chartoumpekis
Apostolos Zaravinos
Panos G Ziros
Ralitsa P Iskrenova
Agathoklis I Psyrogiannis
Venetsana E Kyriazopoulou
Ioannis G Habeos
author_facet Dionysios V Chartoumpekis
Apostolos Zaravinos
Panos G Ziros
Ralitsa P Iskrenova
Agathoklis I Psyrogiannis
Venetsana E Kyriazopoulou
Ioannis G Habeos
author_sort Dionysios V Chartoumpekis
title Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_short Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_full Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_fullStr Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_full_unstemmed Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
title_sort differential expression of micrornas in adipose tissue after long-term high-fat diet-induced obesity in mice.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/570c2d09f4ba4b4e994bc22bc6e327cd
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