Measuring and mitigating PCR bias in microbiota datasets.
PCR amplification plays an integral role in the measurement of mixed microbial communities via high-throughput DNA sequencing of the 16S ribosomal RNA (rRNA) gene. Yet PCR is also known to introduce multiple forms of bias in 16S rRNA studies. Here we present a paired modeling and experimental approa...
Guardado en:
Autores principales: | Justin D Silverman, Rachael J Bloom, Sharon Jiang, Heather K Durand, Eric Dallow, Sayan Mukherjee, Lawrence A David |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/571577d34c1f4bfa89db5707c223535e |
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