Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes

Objective To explore the mechanism of annexin A6 (AnxA6) regulating neural progenitor cells (NPCs) to differentiate into oligodendrocytes (OLs). Methods AnxA6 gene knockout and AnxA6 overexpression viral vectors were constructed in freshly isolated and cultured NPCs, and then qPCR, Western blotting,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	LE Yifan, FAN Ruicheng, XU Yang, LI Hongli
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Office of Journal of Third Military Medical University 2021
Materias:	annexina6 neural progenitor cells oligodendrocytes ca2+ cell differentiation myelination Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/571a9650ee2c4a6180655995297426c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:571a9650ee2c4a6180655995297426c3
record_format	dspace
spelling	oai:doaj.org-article:571a9650ee2c4a6180655995297426c32021-11-12T04:55:31ZMechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes10.16016/j.1000-5404.2021071021000-5404https://doaj.org/article/571a9650ee2c4a6180655995297426c32021-11-01T00:00:00Zhttp://aammt.tmmu.edu.cn/Upload/rhtml/202107102.htmhttps://doaj.org/toc/1000-5404Objective To explore the mechanism of annexin A6 (AnxA6) regulating neural progenitor cells (NPCs) to differentiate into oligodendrocytes (OLs). Methods AnxA6 gene knockout and AnxA6 overexpression viral vectors were constructed in freshly isolated and cultured NPCs, and then qPCR, Western blotting, immunofluorescence staining and calcium imaging in living cells were used to detect the changes of AnxA6 knockout and overexpression on the expression of transcription factors Olig2, Hes1 and Ascl1 in NPCs differentiation in vitro, to observe the dynamic changes of intracellular Ca2+, and to evaluate the effect of AnxA6 on NPC differentiation and maturation. Results Compared with the normal control group, there was no obvious positive band of AnxA6 protein in the knockdown group (P < 0.05), but the OD value of the protein band in the overexpression group was significantly increased (P < 0.01). Under the resting state of NPCs (differentiation for 1 d), the frequency and amplitude of spontaneous oscillation of intracellular calcium ions were higher than those in the control group and the knockdown group. In the NPCs cells differentiated for 3 d, the mRNA level of Olig2 was increased and those of Hes1 and Ascl1 were decreased in the AnxA6 overexpression group. The percentage of Olig2 positive cells and the immunofluorescence signal intensity of CNPase were higher than those in the control group and knockdown group, and the up-regulated expression of the myelin formation-related factor Vimentin and Fyn mRNA was also elevated (P < 0.05). Conclusion Over expression of AnxA6 not only enhances the dynamic changes of Ca2+ in NPCs at the early stage of differentiation, but also promotes the expression of directed transcription factors in OLs and accelerates the formation and myelin sheath.LE YifanFAN RuichengXU YangLI HongliLI HongliEditorial Office of Journal of Third Military Medical Universityarticleannexina6neural progenitor cellsoligodendrocytesca2+cell differentiationmyelinationMedicine (General)R5-920ZHDi-san junyi daxue xuebao, Vol 43, Iss 21, Pp 2343-2349 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	annexina6 neural progenitor cells oligodendrocytes ca2+ cell differentiation myelination Medicine (General) R5-920
spellingShingle	annexina6 neural progenitor cells oligodendrocytes ca2+ cell differentiation myelination Medicine (General) R5-920 LE Yifan FAN Ruicheng XU Yang LI Hongli LI Hongli Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
description	Objective To explore the mechanism of annexin A6 (AnxA6) regulating neural progenitor cells (NPCs) to differentiate into oligodendrocytes (OLs). Methods AnxA6 gene knockout and AnxA6 overexpression viral vectors were constructed in freshly isolated and cultured NPCs, and then qPCR, Western blotting, immunofluorescence staining and calcium imaging in living cells were used to detect the changes of AnxA6 knockout and overexpression on the expression of transcription factors Olig2, Hes1 and Ascl1 in NPCs differentiation in vitro, to observe the dynamic changes of intracellular Ca2+, and to evaluate the effect of AnxA6 on NPC differentiation and maturation. Results Compared with the normal control group, there was no obvious positive band of AnxA6 protein in the knockdown group (P < 0.05), but the OD value of the protein band in the overexpression group was significantly increased (P < 0.01). Under the resting state of NPCs (differentiation for 1 d), the frequency and amplitude of spontaneous oscillation of intracellular calcium ions were higher than those in the control group and the knockdown group. In the NPCs cells differentiated for 3 d, the mRNA level of Olig2 was increased and those of Hes1 and Ascl1 were decreased in the AnxA6 overexpression group. The percentage of Olig2 positive cells and the immunofluorescence signal intensity of CNPase were higher than those in the control group and knockdown group, and the up-regulated expression of the myelin formation-related factor Vimentin and Fyn mRNA was also elevated (P < 0.05). Conclusion Over expression of AnxA6 not only enhances the dynamic changes of Ca2+ in NPCs at the early stage of differentiation, but also promotes the expression of directed transcription factors in OLs and accelerates the formation and myelin sheath.
format	article
author	LE Yifan FAN Ruicheng XU Yang LI Hongli LI Hongli
author_facet	LE Yifan FAN Ruicheng XU Yang LI Hongli LI Hongli
author_sort	LE Yifan
title	Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
title_short	Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
title_full	Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
title_fullStr	Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
title_full_unstemmed	Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
title_sort	mechanism of annexin a6 promoting differentiation and development of neural progenitor cells into oligodendrocytes
publisher	Editorial Office of Journal of Third Military Medical University
publishDate	2021
url	https://doaj.org/article/571a9650ee2c4a6180655995297426c3
work_keys_str_mv	AT leyifan mechanismofannexina6promotingdifferentiationanddevelopmentofneuralprogenitorcellsintooligodendrocytes AT fanruicheng mechanismofannexina6promotingdifferentiationanddevelopmentofneuralprogenitorcellsintooligodendrocytes AT xuyang mechanismofannexina6promotingdifferentiationanddevelopmentofneuralprogenitorcellsintooligodendrocytes AT lihongli mechanismofannexina6promotingdifferentiationanddevelopmentofneuralprogenitorcellsintooligodendrocytes AT lihongli mechanismofannexina6promotingdifferentiationanddevelopmentofneuralprogenitorcellsintooligodendrocytes
_version_	1718431169340506112

Mechanism of annexin A6 promoting differentiation and development of neural progenitor cells into oligodendrocytes

Ejemplares similares