Automated single-molecule imaging in living cells

Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR dynamics in response to various stimuli.

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Detalles Bibliográficos
Autores principales:	Masato Yasui, Michio Hiroshima, Jun Kozuka, Yasushi Sako, Masahiro Ueda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5723c18bb18a45c18246cb484856bf35
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spelling	oai:doaj.org-article:5723c18bb18a45c18246cb484856bf352021-12-02T17:31:16ZAutomated single-molecule imaging in living cells10.1038/s41467-018-05524-72041-1723https://doaj.org/article/5723c18bb18a45c18246cb484856bf352018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05524-7https://doaj.org/toc/2041-1723Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR dynamics in response to various stimuli.Masato YasuiMichio HiroshimaJun KozukaYasushi SakoMasahiro UedaNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Masato Yasui Michio Hiroshima Jun Kozuka Yasushi Sako Masahiro Ueda Automated single-molecule imaging in living cells
description	Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR dynamics in response to various stimuli.
format	article
author	Masato Yasui Michio Hiroshima Jun Kozuka Yasushi Sako Masahiro Ueda
author_facet	Masato Yasui Michio Hiroshima Jun Kozuka Yasushi Sako Masahiro Ueda
author_sort	Masato Yasui
title	Automated single-molecule imaging in living cells
title_short	Automated single-molecule imaging in living cells
title_full	Automated single-molecule imaging in living cells
title_fullStr	Automated single-molecule imaging in living cells
title_full_unstemmed	Automated single-molecule imaging in living cells
title_sort	automated single-molecule imaging in living cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5723c18bb18a45c18246cb484856bf35
work_keys_str_mv	AT masatoyasui automatedsinglemoleculeimaginginlivingcells AT michiohiroshima automatedsinglemoleculeimaginginlivingcells AT junkozuka automatedsinglemoleculeimaginginlivingcells AT yasushisako automatedsinglemoleculeimaginginlivingcells AT masahiroueda automatedsinglemoleculeimaginginlivingcells
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Automated single-molecule imaging in living cells

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