Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.

The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then 'scratched' with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration an...

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Autores principales:	Mohammadali E Alishahedani, Manoj Yadav, Katelyn J McCann, Portia Gough, Carlos R Castillo, Jobel Matriz, Ian A Myles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/572782209e2e45908963862d4bbe19fc
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spelling	oai:doaj.org-article:572782209e2e45908963862d4bbe19fc2021-12-02T20:10:21ZTherapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.1932-620310.1371/journal.pone.0253669https://doaj.org/article/572782209e2e45908963862d4bbe19fc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253669https://doaj.org/toc/1932-6203The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then 'scratched' with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to 'heal' the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.Mohammadali E AlishahedaniManoj YadavKatelyn J McCannPortia GoughCarlos R CastilloJobel MatrizIan A MylesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253669 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammadali E Alishahedani Manoj Yadav Katelyn J McCann Portia Gough Carlos R Castillo Jobel Matriz Ian A Myles Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
description	The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then 'scratched' with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to 'heal' the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.
format	article
author	Mohammadali E Alishahedani Manoj Yadav Katelyn J McCann Portia Gough Carlos R Castillo Jobel Matriz Ian A Myles
author_facet	Mohammadali E Alishahedani Manoj Yadav Katelyn J McCann Portia Gough Carlos R Castillo Jobel Matriz Ian A Myles
author_sort	Mohammadali E Alishahedani
title	Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
title_short	Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
title_full	Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
title_fullStr	Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
title_full_unstemmed	Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
title_sort	therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/572782209e2e45908963862d4bbe19fc
work_keys_str_mv	AT mohammadaliealishahedani therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT manojyadav therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT katelynjmccann therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT portiagough therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT carlosrcastillo therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT jobelmatriz therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing AT ianamyles therapeuticcandidatesforkeloidscarsidentifiedbyqualitativereviewofscratchassayresearchforwoundhealing
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Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.

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