Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.

Staphylococcus aureus is a successful human pathogen that has developed several approaches to evade the immune system, including resistance strategies to prevent oxidative killing by immune cells. One mechanism by which this evasion occurs is by production of superoxide dismutase enzymes, which requ...

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Autores principales:	Luke D Handke, Julio C Hawkins, Alita A Miller, Kathrin U Jansen, Annaliesa S Anderson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/572d78d3434a4dd195238bdfef613cb6
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spelling	oai:doaj.org-article:572d78d3434a4dd195238bdfef613cb62021-11-18T08:49:15ZRegulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.1932-620310.1371/journal.pone.0077874https://doaj.org/article/572d78d3434a4dd195238bdfef613cb62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24205007/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Staphylococcus aureus is a successful human pathogen that has developed several approaches to evade the immune system, including resistance strategies to prevent oxidative killing by immune cells. One mechanism by which this evasion occurs is by production of superoxide dismutase enzymes, which require manganese as a cofactor. Manganese is acquired by the manganese transporter MntABC. One component of this operon, MntC, has been proposed as a potential vaccine candidate due to its early in vivo expression and its ability to provide protection in preclinical models of staphylococcal infection. In the current study, we interrogate the role of this protein in protecting S. aureus from oxidative stress. We demonstrate that mutation of mntC in a number of invasive S. aureus clinical isolates results in increased sensitivity to oxidative stress. In addition, we show that while downregulation of mntC transcription is triggered upon exposure to physiological concentrations of manganese, MntC protein is still present on the bacterial surface at these same concentrations. Taken together, these results provide insight into the role of this antigen for the pathogen.Luke D HandkeJulio C HawkinsAlita A MillerKathrin U JansenAnnaliesa S AndersonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77874 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luke D Handke Julio C Hawkins Alita A Miller Kathrin U Jansen Annaliesa S Anderson Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
description	Staphylococcus aureus is a successful human pathogen that has developed several approaches to evade the immune system, including resistance strategies to prevent oxidative killing by immune cells. One mechanism by which this evasion occurs is by production of superoxide dismutase enzymes, which require manganese as a cofactor. Manganese is acquired by the manganese transporter MntABC. One component of this operon, MntC, has been proposed as a potential vaccine candidate due to its early in vivo expression and its ability to provide protection in preclinical models of staphylococcal infection. In the current study, we interrogate the role of this protein in protecting S. aureus from oxidative stress. We demonstrate that mutation of mntC in a number of invasive S. aureus clinical isolates results in increased sensitivity to oxidative stress. In addition, we show that while downregulation of mntC transcription is triggered upon exposure to physiological concentrations of manganese, MntC protein is still present on the bacterial surface at these same concentrations. Taken together, these results provide insight into the role of this antigen for the pathogen.
format	article
author	Luke D Handke Julio C Hawkins Alita A Miller Kathrin U Jansen Annaliesa S Anderson
author_facet	Luke D Handke Julio C Hawkins Alita A Miller Kathrin U Jansen Annaliesa S Anderson
author_sort	Luke D Handke
title	Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
title_short	Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
title_full	Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
title_fullStr	Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
title_full_unstemmed	Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.
title_sort	regulation of staphylococcus aureus mntc expression and its role in response to oxidative stress.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/572d78d3434a4dd195238bdfef613cb6
work_keys_str_mv	AT lukedhandke regulationofstaphylococcusaureusmntcexpressionanditsroleinresponsetooxidativestress AT juliochawkins regulationofstaphylococcusaureusmntcexpressionanditsroleinresponsetooxidativestress AT alitaamiller regulationofstaphylococcusaureusmntcexpressionanditsroleinresponsetooxidativestress AT kathrinujansen regulationofstaphylococcusaureusmntcexpressionanditsroleinresponsetooxidativestress AT annaliesasanderson regulationofstaphylococcusaureusmntcexpressionanditsroleinresponsetooxidativestress
_version_	1718421262040039424

Regulation of Staphylococcus aureus MntC expression and its role in response to oxidative stress.

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