Comparative analysis of plasmids in the genus Listeria.

<h4>Background</h4>We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolut...

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Autores principales:	Carsten Kuenne, Sonja Voget, Jordan Pischimarov, Sebastian Oehm, Alexander Goesmann, Rolf Daniel, Torsten Hain, Trinad Chakraborty
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/572e6c86c85f479ca7b3db4030780c37
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spelling	oai:doaj.org-article:572e6c86c85f479ca7b3db4030780c372021-11-18T06:35:29ZComparative analysis of plasmids in the genus Listeria.1932-620310.1371/journal.pone.0012511https://doaj.org/article/572e6c86c85f479ca7b3db4030780c372010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20824078/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolutionary background.<h4>Principal findings</h4>All analysed plasmids share a common replicon-type related to theta-replicating plasmid pAMbeta1. Nonetheless plasmids could be broadly divided into two distinct groups based on replicon diversity and the genetic content of the respective plasmid groups. Listeria plasmids are characterized by the presence of a large number of diverse mobile genetic elements and a commonly occurring translesion DNA polymerase both of which have probably contributed to the evolution of these plasmids. We detected small non-coding RNAs on some plasmids that were homologous to those present on the chromosome of L. monocytogenes EGD-e. Multiple genes involved in heavy metal resistance (cadmium, copper, arsenite) as well as multidrug efflux (MDR, SMR, MATE) were detected on all listerial plasmids. These factors promote bacterial growth and survival in the environment and may have been acquired as a result of selective pressure due to the use of disinfectants in food processing environments. MDR efflux pumps have also recently been shown to promote transport of cyclic diadenosine monophosphate (c-di-AMP) as a secreted molecule able to trigger a cytosolic host immune response following infection.<h4>Conclusions</h4>The comparative analysis of 14 plasmids of genus Listeria implied the existence of a common ancestor. Ubiquitously-occurring MDR genes on plasmids and their role in listerial infection now deserve further attention.Carsten KuenneSonja VogetJordan PischimarovSebastian OehmAlexander GoesmannRolf DanielTorsten HainTrinad ChakrabortyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carsten Kuenne Sonja Voget Jordan Pischimarov Sebastian Oehm Alexander Goesmann Rolf Daniel Torsten Hain Trinad Chakraborty Comparative analysis of plasmids in the genus Listeria.
description	<h4>Background</h4>We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolutionary background.<h4>Principal findings</h4>All analysed plasmids share a common replicon-type related to theta-replicating plasmid pAMbeta1. Nonetheless plasmids could be broadly divided into two distinct groups based on replicon diversity and the genetic content of the respective plasmid groups. Listeria plasmids are characterized by the presence of a large number of diverse mobile genetic elements and a commonly occurring translesion DNA polymerase both of which have probably contributed to the evolution of these plasmids. We detected small non-coding RNAs on some plasmids that were homologous to those present on the chromosome of L. monocytogenes EGD-e. Multiple genes involved in heavy metal resistance (cadmium, copper, arsenite) as well as multidrug efflux (MDR, SMR, MATE) were detected on all listerial plasmids. These factors promote bacterial growth and survival in the environment and may have been acquired as a result of selective pressure due to the use of disinfectants in food processing environments. MDR efflux pumps have also recently been shown to promote transport of cyclic diadenosine monophosphate (c-di-AMP) as a secreted molecule able to trigger a cytosolic host immune response following infection.<h4>Conclusions</h4>The comparative analysis of 14 plasmids of genus Listeria implied the existence of a common ancestor. Ubiquitously-occurring MDR genes on plasmids and their role in listerial infection now deserve further attention.
format	article
author	Carsten Kuenne Sonja Voget Jordan Pischimarov Sebastian Oehm Alexander Goesmann Rolf Daniel Torsten Hain Trinad Chakraborty
author_facet	Carsten Kuenne Sonja Voget Jordan Pischimarov Sebastian Oehm Alexander Goesmann Rolf Daniel Torsten Hain Trinad Chakraborty
author_sort	Carsten Kuenne
title	Comparative analysis of plasmids in the genus Listeria.
title_short	Comparative analysis of plasmids in the genus Listeria.
title_full	Comparative analysis of plasmids in the genus Listeria.
title_fullStr	Comparative analysis of plasmids in the genus Listeria.
title_full_unstemmed	Comparative analysis of plasmids in the genus Listeria.
title_sort	comparative analysis of plasmids in the genus listeria.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/572e6c86c85f479ca7b3db4030780c37
work_keys_str_mv	AT carstenkuenne comparativeanalysisofplasmidsinthegenuslisteria AT sonjavoget comparativeanalysisofplasmidsinthegenuslisteria AT jordanpischimarov comparativeanalysisofplasmidsinthegenuslisteria AT sebastianoehm comparativeanalysisofplasmidsinthegenuslisteria AT alexandergoesmann comparativeanalysisofplasmidsinthegenuslisteria AT rolfdaniel comparativeanalysisofplasmidsinthegenuslisteria AT torstenhain comparativeanalysisofplasmidsinthegenuslisteria AT trinadchakraborty comparativeanalysisofplasmidsinthegenuslisteria
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Comparative analysis of plasmids in the genus Listeria.

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