QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.

<h4>Background</h4>The human QKI gene, called quaking homolog, KH domain RNA binding (mouse), is a candidate gene for schizophrenia encoding an RNA-binding protein. This gene was shown to be essential for myelination in oligodendrocytes. QKI is also highly expressed in astrocytes, but it...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lin Jiang, Peter Saetre, Katarzyna J Radomska, Elena Jazin, Eva Lindholm Carlström
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5731ee0d823345e5a308daa7c60dc01a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5731ee0d823345e5a308daa7c60dc01a
record_format	dspace
spelling	oai:doaj.org-article:5731ee0d823345e5a308daa7c60dc01a2021-11-18T07:03:51ZQKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.1932-620310.1371/journal.pone.0013079https://doaj.org/article/5731ee0d823345e5a308daa7c60dc01a2010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20927331/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The human QKI gene, called quaking homolog, KH domain RNA binding (mouse), is a candidate gene for schizophrenia encoding an RNA-binding protein. This gene was shown to be essential for myelination in oligodendrocytes. QKI is also highly expressed in astrocytes, but its function in these cells is not known.<h4>Methods/principal findings</h4>We studied the effect of small interference RNA (siRNA)-mediated QKI depletion on global gene expression in human astrocyte glioma cells. Microarray measurements were confirmed with real-time quantitative polymerase chain reaction (qPCR). The presence of QKI binding sites (QRE) was assessed by a bioinformatic approach. Viability and cell morphology were also studied. The most significant alteration after QKI silencing was the decreased expression of genes involved in interferon (IFN) induction (P = 6.3E-10), including IFIT1, IFIT2, MX1, MX2, G1P2, G1P3, GBP1 and IFIH1. All eight genes were down-regulated after silencing of the splice variant QKI-7, but were not affected by QKI-5 silencing. Interestingly, four of them were up-regulated after treatment with the antipsychotic agent haloperidol that also resulted in increased QKI-7 mRNA levels.<h4>Conclusions/significance</h4>The coordinated expression of QKI-7 splice variant and IFN-related genes supports the idea that this particular splice variant has specific functions in astrocytes. Furthermore, a role of QKI-7 as a regulator of an inflammatory gene pathway in astrocytes is suggested. This hypothesis is well in line with growing experimental evidence on the role of inflammatory components in schizophrenia.Lin JiangPeter SaetreKatarzyna J RadomskaElena JazinEva Lindholm CarlströmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Jiang Peter Saetre Katarzyna J Radomska Elena Jazin Eva Lindholm Carlström QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
description	<h4>Background</h4>The human QKI gene, called quaking homolog, KH domain RNA binding (mouse), is a candidate gene for schizophrenia encoding an RNA-binding protein. This gene was shown to be essential for myelination in oligodendrocytes. QKI is also highly expressed in astrocytes, but its function in these cells is not known.<h4>Methods/principal findings</h4>We studied the effect of small interference RNA (siRNA)-mediated QKI depletion on global gene expression in human astrocyte glioma cells. Microarray measurements were confirmed with real-time quantitative polymerase chain reaction (qPCR). The presence of QKI binding sites (QRE) was assessed by a bioinformatic approach. Viability and cell morphology were also studied. The most significant alteration after QKI silencing was the decreased expression of genes involved in interferon (IFN) induction (P = 6.3E-10), including IFIT1, IFIT2, MX1, MX2, G1P2, G1P3, GBP1 and IFIH1. All eight genes were down-regulated after silencing of the splice variant QKI-7, but were not affected by QKI-5 silencing. Interestingly, four of them were up-regulated after treatment with the antipsychotic agent haloperidol that also resulted in increased QKI-7 mRNA levels.<h4>Conclusions/significance</h4>The coordinated expression of QKI-7 splice variant and IFN-related genes supports the idea that this particular splice variant has specific functions in astrocytes. Furthermore, a role of QKI-7 as a regulator of an inflammatory gene pathway in astrocytes is suggested. This hypothesis is well in line with growing experimental evidence on the role of inflammatory components in schizophrenia.
format	article
author	Lin Jiang Peter Saetre Katarzyna J Radomska Elena Jazin Eva Lindholm Carlström
author_facet	Lin Jiang Peter Saetre Katarzyna J Radomska Elena Jazin Eva Lindholm Carlström
author_sort	Lin Jiang
title	QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
title_short	QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
title_full	QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
title_fullStr	QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
title_full_unstemmed	QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
title_sort	qki-7 regulates expression of interferon-related genes in human astrocyte glioma cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/5731ee0d823345e5a308daa7c60dc01a
work_keys_str_mv	AT linjiang qki7regulatesexpressionofinterferonrelatedgenesinhumanastrocytegliomacells AT petersaetre qki7regulatesexpressionofinterferonrelatedgenesinhumanastrocytegliomacells AT katarzynajradomska qki7regulatesexpressionofinterferonrelatedgenesinhumanastrocytegliomacells AT elenajazin qki7regulatesexpressionofinterferonrelatedgenesinhumanastrocytegliomacells AT evalindholmcarlstrom qki7regulatesexpressionofinterferonrelatedgenesinhumanastrocytegliomacells
_version_	1718423977597075456

QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.

Ejemplares similares