Neural regulation in tooth regeneration of Ambystoma mexicanum

Abstract The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here,...

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Autores principales:	Aki Makanae, Yuki Tajika, Koki Nishimura, Nanami Saito, Jun-ichi Tanaka, Akira Satoh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5732d5ad08784ac8b667310b1567d290
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spelling	oai:doaj.org-article:5732d5ad08784ac8b667310b1567d2902021-12-02T17:52:43ZNeural regulation in tooth regeneration of Ambystoma mexicanum10.1038/s41598-020-66142-22045-2322https://doaj.org/article/5732d5ad08784ac8b667310b1567d2902020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66142-2https://doaj.org/toc/2045-2322Abstract The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.Aki MakanaeYuki TajikaKoki NishimuraNanami SaitoJun-ichi TanakaAkira SatohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aki Makanae Yuki Tajika Koki Nishimura Nanami Saito Jun-ichi Tanaka Akira Satoh Neural regulation in tooth regeneration of Ambystoma mexicanum
description	Abstract The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.
format	article
author	Aki Makanae Yuki Tajika Koki Nishimura Nanami Saito Jun-ichi Tanaka Akira Satoh
author_facet	Aki Makanae Yuki Tajika Koki Nishimura Nanami Saito Jun-ichi Tanaka Akira Satoh
author_sort	Aki Makanae
title	Neural regulation in tooth regeneration of Ambystoma mexicanum
title_short	Neural regulation in tooth regeneration of Ambystoma mexicanum
title_full	Neural regulation in tooth regeneration of Ambystoma mexicanum
title_fullStr	Neural regulation in tooth regeneration of Ambystoma mexicanum
title_full_unstemmed	Neural regulation in tooth regeneration of Ambystoma mexicanum
title_sort	neural regulation in tooth regeneration of ambystoma mexicanum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5732d5ad08784ac8b667310b1567d290
work_keys_str_mv	AT akimakanae neuralregulationintoothregenerationofambystomamexicanum AT yukitajika neuralregulationintoothregenerationofambystomamexicanum AT kokinishimura neuralregulationintoothregenerationofambystomamexicanum AT nanamisaito neuralregulationintoothregenerationofambystomamexicanum AT junichitanaka neuralregulationintoothregenerationofambystomamexicanum AT akirasatoh neuralregulationintoothregenerationofambystomamexicanum
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Neural regulation in tooth regeneration of Ambystoma mexicanum

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