Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. E...

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Autores principales:	Jolanta Lis-Kuberka, Paulina Kubik, Agnieszka Chrobak, Jarosław Pająk, Anna Chełmońska-Soyta, Magdalena Orczyk-Pawiłowicz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometriosis fertility disorders molecular status of fibronectin fibrin Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/57333a0be7f04b398f534741996659c4
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id	oai:doaj.org-article:57333a0be7f04b398f534741996659c4
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spelling	oai:doaj.org-article:57333a0be7f04b398f534741996659c42021-11-11T16:53:18ZFibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders10.3390/ijms2221114101422-00671661-6596https://doaj.org/article/57333a0be7f04b398f534741996659c42021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11410https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. ELISA and SDS-agarose immunoblotting were applied to determine the fibronectin concentration and presence and occurrence of soluble FN-fibrin complexes in the blood plasma of women with endometriosis (n = 38), fertility disorders (n = 28) and the healthy group (n = 25). The concentration of fibronectin in the blood plasma of women with endometriosis (292.61 ± 96.17 mg/L) and fertility disorders (287.53 ± 122.68 mg/L) was significantly higher than in the normal group (226.55 ± 91.98 mg/L). The presence of FN-fibrin complexes of 750, 1000, 1300, 1600 and 1900 kDa in the plasma of women with endometriosis and fertility disorders was shown. The presence of FN-fibrin complexes with a molecular mass of more than 1300 kDa in women with endometriosis and infertility and the complete absence of these complexes in healthy women may indicate an increased and chronic activation of coagulation mechanisms in these patients. The presence of complexes of high molecular mass may be one of the biomarkers of fertility disorders in women.Jolanta Lis-KuberkaPaulina KubikAgnieszka ChrobakJarosław PająkAnna Chełmońska-SoytaMagdalena Orczyk-PawiłowiczMDPI AGarticleendometriosisfertility disordersmolecular status of fibronectinfibrinBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11410, p 11410 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometriosis fertility disorders molecular status of fibronectin fibrin Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	endometriosis fertility disorders molecular status of fibronectin fibrin Biology (General) QH301-705.5 Chemistry QD1-999 Jolanta Lis-Kuberka Paulina Kubik Agnieszka Chrobak Jarosław Pająk Anna Chełmońska-Soyta Magdalena Orczyk-Pawiłowicz Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
description	The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. ELISA and SDS-agarose immunoblotting were applied to determine the fibronectin concentration and presence and occurrence of soluble FN-fibrin complexes in the blood plasma of women with endometriosis (n = 38), fertility disorders (n = 28) and the healthy group (n = 25). The concentration of fibronectin in the blood plasma of women with endometriosis (292.61 ± 96.17 mg/L) and fertility disorders (287.53 ± 122.68 mg/L) was significantly higher than in the normal group (226.55 ± 91.98 mg/L). The presence of FN-fibrin complexes of 750, 1000, 1300, 1600 and 1900 kDa in the plasma of women with endometriosis and fertility disorders was shown. The presence of FN-fibrin complexes with a molecular mass of more than 1300 kDa in women with endometriosis and infertility and the complete absence of these complexes in healthy women may indicate an increased and chronic activation of coagulation mechanisms in these patients. The presence of complexes of high molecular mass may be one of the biomarkers of fertility disorders in women.
format	article
author	Jolanta Lis-Kuberka Paulina Kubik Agnieszka Chrobak Jarosław Pająk Anna Chełmońska-Soyta Magdalena Orczyk-Pawiłowicz
author_facet	Jolanta Lis-Kuberka Paulina Kubik Agnieszka Chrobak Jarosław Pająk Anna Chełmońska-Soyta Magdalena Orczyk-Pawiłowicz
author_sort	Jolanta Lis-Kuberka
title	Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
title_short	Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
title_full	Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
title_fullStr	Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
title_full_unstemmed	Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders
title_sort	fibronectin molecular status in plasma of women with endometriosis and fertility disorders
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/57333a0be7f04b398f534741996659c4
work_keys_str_mv	AT jolantaliskuberka fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders AT paulinakubik fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders AT agnieszkachrobak fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders AT jarosławpajak fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders AT annachełmonskasoyta fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders AT magdalenaorczykpawiłowicz fibronectinmolecularstatusinplasmaofwomenwithendometriosisandfertilitydisorders
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Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

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