MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites

The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measu...

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Autores principales:	Claire Shyu, Sonja Elsaid, Peter Truong, Sofia Chavez, Bernard Le Foll
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	insula gamma-aminobutyric acid GABA LCModel MEGA-PRESS PRESS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/5734595edaeb47c59e6e84174fe60112
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spelling	oai:doaj.org-article:5734595edaeb47c59e6e84174fe601122021-11-25T16:59:17ZMR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites10.3390/brainsci111115382076-3425https://doaj.org/article/5734595edaeb47c59e6e84174fe601122021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3425/11/11/1538https://doaj.org/toc/2076-3425The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measurements in the insula on a 3T scanner. We measure N-acetylaspartate + N-acetylaspartylglutamate (tNAA), creatine + phosphocreatine (tCr), glycerophosphocholine + phosphocholine (tCho), myo-inositol (Ins), glutamate + glutamine (Glx), and γ-aminobutyric acid (GABA) in one cohort using a j-edited MEGA-PRESS sequence. We measure tNAA, tCr, tCho, Ins, and Glx in another cohort with a standard short-TE PRESS sequence as a reference for the reproducibility metrics. All participants were scanned 4 times identically: 2 back-to-back scans each day, on 2 days. Preprocessing was done using LCModel and Gannet. Reproducibility was determined using Pearson’s <i>r</i>, intraclass-correlation coefficients (ICC), coefficients of variation (CV%), and Bland–Altman plots. A MEGA-PRESS protocol requiring averaged results over two 6:45-min scans yielded reproducible GABA measurements (CV% = 7.15%). This averaging also yielded reproducibility metrics comparable to those from PRESS for the other metabolites. Voxel placement inconsistencies did not affect reproducibility, and no sex differences were found. The data suggest that MEGA-PRESS can reliably measure standard metabolites and GABA in the insula.Claire ShyuSonja ElsaidPeter TruongSofia ChavezBernard Le FollMDPI AGarticleinsulagamma-aminobutyric acidGABALCModelMEGA-PRESSPRESSNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBrain Sciences, Vol 11, Iss 1538, p 1538 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	insula gamma-aminobutyric acid GABA LCModel MEGA-PRESS PRESS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	insula gamma-aminobutyric acid GABA LCModel MEGA-PRESS PRESS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
description	The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measurements in the insula on a 3T scanner. We measure N-acetylaspartate + N-acetylaspartylglutamate (tNAA), creatine + phosphocreatine (tCr), glycerophosphocholine + phosphocholine (tCho), myo-inositol (Ins), glutamate + glutamine (Glx), and γ-aminobutyric acid (GABA) in one cohort using a j-edited MEGA-PRESS sequence. We measure tNAA, tCr, tCho, Ins, and Glx in another cohort with a standard short-TE PRESS sequence as a reference for the reproducibility metrics. All participants were scanned 4 times identically: 2 back-to-back scans each day, on 2 days. Preprocessing was done using LCModel and Gannet. Reproducibility was determined using Pearson’s <i>r</i>, intraclass-correlation coefficients (ICC), coefficients of variation (CV%), and Bland–Altman plots. A MEGA-PRESS protocol requiring averaged results over two 6:45-min scans yielded reproducible GABA measurements (CV% = 7.15%). This averaging also yielded reproducibility metrics comparable to those from PRESS for the other metabolites. Voxel placement inconsistencies did not affect reproducibility, and no sex differences were found. The data suggest that MEGA-PRESS can reliably measure standard metabolites and GABA in the insula.
format	article
author	Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll
author_facet	Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll
author_sort	Claire Shyu
title	MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
title_short	MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
title_full	MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
title_fullStr	MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
title_full_unstemmed	MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
title_sort	mr spectroscopy of the insula: within- and between-session reproducibility of mega-press measurements of gaba+ and other metabolites
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5734595edaeb47c59e6e84174fe60112
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MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites

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