GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana

Abstract Drought transcriptome analysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize (Zea mays), several genes, including a G-...

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Autores principales:	Venkategowda Ramegowda, Upinder Singh Gill, Palaiyur Nanjappan Sivalingam, Aarti Gupta, Chirag Gupta, Geetha Govind, Karaba N. Nataraja, Andy Pereira, Makarla Udayakumar, Kirankumar S. Mysore, Muthappa Senthil-Kumar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5737a61a5c28424b9eacea4bbdb48801
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spelling	oai:doaj.org-article:5737a61a5c28424b9eacea4bbdb488012021-12-02T12:30:44ZGBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana10.1038/s41598-017-09542-12045-2322https://doaj.org/article/5737a61a5c28424b9eacea4bbdb488012017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09542-1https://doaj.org/toc/2045-2322Abstract Drought transcriptome analysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize (Zea mays), several genes, including a G-BOX BINDING FACTOR 3 (GBF3) were identified as candidate drought stress response genes and the role of GBF3 in drought tolerance was studied in Arabidopsis thaliana. Overexpression of both EcGBF3 and AtGBF3 in A. thaliana resulted in improved tolerance to osmotic stress, salinity and drought stress in addition to conferring insensitivity to ABA. Conversely, loss of function of this gene increased the sensitivity of A. thaliana plants to drought stress. EcGBF3 transgenic A. thaliana results also suggest that drought tolerance of sensitive plants can be improved by transferring genes from far related crops like finger millet. Our results demonstrate the role of GBF3 in imparting drought tolerance in A. thaliana and indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant species.Venkategowda RamegowdaUpinder Singh GillPalaiyur Nanjappan SivalingamAarti GuptaChirag GuptaGeetha GovindKaraba N. NatarajaAndy PereiraMakarla UdayakumarKirankumar S. MysoreMuthappa Senthil-KumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Venkategowda Ramegowda Upinder Singh Gill Palaiyur Nanjappan Sivalingam Aarti Gupta Chirag Gupta Geetha Govind Karaba N. Nataraja Andy Pereira Makarla Udayakumar Kirankumar S. Mysore Muthappa Senthil-Kumar GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
description	Abstract Drought transcriptome analysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize (Zea mays), several genes, including a G-BOX BINDING FACTOR 3 (GBF3) were identified as candidate drought stress response genes and the role of GBF3 in drought tolerance was studied in Arabidopsis thaliana. Overexpression of both EcGBF3 and AtGBF3 in A. thaliana resulted in improved tolerance to osmotic stress, salinity and drought stress in addition to conferring insensitivity to ABA. Conversely, loss of function of this gene increased the sensitivity of A. thaliana plants to drought stress. EcGBF3 transgenic A. thaliana results also suggest that drought tolerance of sensitive plants can be improved by transferring genes from far related crops like finger millet. Our results demonstrate the role of GBF3 in imparting drought tolerance in A. thaliana and indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant species.
format	article
author	Venkategowda Ramegowda Upinder Singh Gill Palaiyur Nanjappan Sivalingam Aarti Gupta Chirag Gupta Geetha Govind Karaba N. Nataraja Andy Pereira Makarla Udayakumar Kirankumar S. Mysore Muthappa Senthil-Kumar
author_facet	Venkategowda Ramegowda Upinder Singh Gill Palaiyur Nanjappan Sivalingam Aarti Gupta Chirag Gupta Geetha Govind Karaba N. Nataraja Andy Pereira Makarla Udayakumar Kirankumar S. Mysore Muthappa Senthil-Kumar
author_sort	Venkategowda Ramegowda
title	GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
title_short	GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
title_full	GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
title_fullStr	GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
title_full_unstemmed	GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
title_sort	gbf3 transcription factor imparts drought tolerance in arabidopsis thaliana
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5737a61a5c28424b9eacea4bbdb48801
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GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana

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