Identification of cbiO Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis

The colonization of Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), has a detrimental effect on the respiratory care of pediatric patients with cystic fibrosis (CF). In addition to being resistant to multiple antibiotics, S. au...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying Liu, Junshu Yang, Michelle Ji, James Phillips, Mark Wylam, Yinduo Ji
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> MRSA biofilm formation <i>cbiO</i> copper ions Medicine R
Acceso en línea:	https://doaj.org/article/5739aaa053d140b2b5a4a2087df98037
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5739aaa053d140b2b5a4a2087df98037
record_format	dspace
spelling	oai:doaj.org-article:5739aaa053d140b2b5a4a2087df980372021-11-25T18:37:38ZIdentification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis10.3390/pathogens101113632076-0817https://doaj.org/article/5739aaa053d140b2b5a4a2087df980372021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1363https://doaj.org/toc/2076-0817The colonization of <i>Staphylococcus aureus</i>, especially methicillin-resistant <i>S. aureus</i> (MRSA), has a detrimental effect on the respiratory care of pediatric patients with cystic fibrosis (CF). In addition to being resistant to multiple antibiotics, <i>S. aureus</i> also has the ability to form biofilms, which makes the infection more difficult to treat and eradicate. In this study, we examined the ability of <i>S. aureus</i> strains isolated from pediatric patients with CF to form biofilms. We screened a transposon mutant library of MRSA and identified a putative cobalt transporter ATP binding domain (<i>cbiO</i>) that is required for biofilm formation. We discovered that deleting <i>cbiO</i> creating a <i>cbiO</i> null mutant in CFSa36 (an MRSA strain isolated from a patient with cystic fibrosis) significantly hinders the ability of CFSa36 to form biofilm. The complementation of <i>cbiO</i> restored the ability of the <i>cbiO</i> deletion mutant to generate biofilm. Interestingly, we revealed that incorporating extra copper ions to the chemically defined medium (CDM) complemented the function of <i>cbiO</i> for biofilm formation in a dose-dependent manner, while the addition of extra iron ions in CDM enhanced the effect of <i>cbiO</i> null mutation on biofilm formation. In addition, neither the addition of certain extra amounts of copper ions nor iron ions in CDM had an impact on bacterial growth. Taken together, our findings suggest that <i>cbiO</i> mediates biofilm formation by affecting the transportation of copper ions in the MRSA CFSa36 strain. This study provides new insights into the molecular basis of biofilm formation by <i>S. aureus</i>.Ying LiuJunshu YangMichelle JiJames PhillipsMark WylamYinduo JiMDPI AGarticle<i>Staphylococcus aureus</i>MRSAbiofilm formation<i>cbiO</i>copper ionsMedicineRENPathogens, Vol 10, Iss 1363, p 1363 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> MRSA biofilm formation <i>cbiO</i> copper ions Medicine R
spellingShingle	<i>Staphylococcus aureus</i> MRSA biofilm formation <i>cbiO</i> copper ions Medicine R Ying Liu Junshu Yang Michelle Ji James Phillips Mark Wylam Yinduo Ji Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
description	The colonization of <i>Staphylococcus aureus</i>, especially methicillin-resistant <i>S. aureus</i> (MRSA), has a detrimental effect on the respiratory care of pediatric patients with cystic fibrosis (CF). In addition to being resistant to multiple antibiotics, <i>S. aureus</i> also has the ability to form biofilms, which makes the infection more difficult to treat and eradicate. In this study, we examined the ability of <i>S. aureus</i> strains isolated from pediatric patients with CF to form biofilms. We screened a transposon mutant library of MRSA and identified a putative cobalt transporter ATP binding domain (<i>cbiO</i>) that is required for biofilm formation. We discovered that deleting <i>cbiO</i> creating a <i>cbiO</i> null mutant in CFSa36 (an MRSA strain isolated from a patient with cystic fibrosis) significantly hinders the ability of CFSa36 to form biofilm. The complementation of <i>cbiO</i> restored the ability of the <i>cbiO</i> deletion mutant to generate biofilm. Interestingly, we revealed that incorporating extra copper ions to the chemically defined medium (CDM) complemented the function of <i>cbiO</i> for biofilm formation in a dose-dependent manner, while the addition of extra iron ions in CDM enhanced the effect of <i>cbiO</i> null mutation on biofilm formation. In addition, neither the addition of certain extra amounts of copper ions nor iron ions in CDM had an impact on bacterial growth. Taken together, our findings suggest that <i>cbiO</i> mediates biofilm formation by affecting the transportation of copper ions in the MRSA CFSa36 strain. This study provides new insights into the molecular basis of biofilm formation by <i>S. aureus</i>.
format	article
author	Ying Liu Junshu Yang Michelle Ji James Phillips Mark Wylam Yinduo Ji
author_facet	Ying Liu Junshu Yang Michelle Ji James Phillips Mark Wylam Yinduo Ji
author_sort	Ying Liu
title	Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
title_short	Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
title_full	Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
title_fullStr	Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
title_full_unstemmed	Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
title_sort	identification of <i>cbio</i> gene critical for biofilm formation by mrsa cfsa36 strain isolated from pediatric patient with cystic fibrosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5739aaa053d140b2b5a4a2087df98037
work_keys_str_mv	AT yingliu identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis AT junshuyang identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis AT michelleji identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis AT jamesphillips identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis AT markwylam identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis AT yinduoji identificationoficbioigenecriticalforbiofilmformationbymrsacfsa36strainisolatedfrompediatricpatientwithcysticfibrosis
_version_	1718410891933777920

Identification of <i>cbiO</i> Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis

Ejemplares similares