Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization

ABSTRACT Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mech...

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Autores principales:	Volker Winstel, Petra Kühner, Ferdinand Salomon, Jesper Larsen, Robert Skov, Wolfgang Hoffmann, Andreas Peschel, Christopher Weidenmaier
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5743d590b37e449ebd3f858f2fe094c9
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spelling	oai:doaj.org-article:5743d590b37e449ebd3f858f2fe094c92021-11-15T15:41:26ZWall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization10.1128/mBio.00632-152150-7511https://doaj.org/article/5743d590b37e449ebd3f858f2fe094c92015-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00632-15https://doaj.org/toc/2150-7511ABSTRACT Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that WTA GlcNAcylation is a pivotal requirement for WTA-dependent attachment of community-acquired methicillin-resistant S. aureus (MRSA) and emerging livestock-associated MRSA to human nasal epithelial cells, even under conditions simulating the nutrient composition and dynamic flow of nasal secretions. Depending on the S. aureus strain, WTA O-GlcNAcylation occurs in either α or β configuration, which have similar capacities to mediate attachment to human nasal epithelial cells, suggesting that many S. aureus strains maintain redundant pathways to ensure appropriate WTA glycosylation. Strikingly, a lack of WTA glycosylation significantly abrogated the ability of MRSA to colonize cotton rat nares in vivo. These results indicate that WTA glycosylation modulates S. aureus nasal colonization and may help to develop new strategies for eradicating S. aureus nasal colonization in the future. IMPORTANCE Nasal colonization by the major human pathogen Staphylococcus aureus is a risk factor for severe endogenous infections and contributes to the spread of this microbe in hospitals and the community. Here, we show that wall teichoic acid (WTA) O-GlcNAcylation is a key factor required for S. aureus nasal colonization. These data provide a mechanistic explanation for the capacity of WTA to modulate S. aureus nasal colonization and may stimulate research activities to establish valuable strategies to eradicate S. aureus nasal colonization in high-risk hospitalized patients and in the general community.Volker WinstelPetra KühnerFerdinand SalomonJesper LarsenRobert SkovWolfgang HoffmannAndreas PeschelChristopher WeidenmaierAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 4 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Volker Winstel Petra Kühner Ferdinand Salomon Jesper Larsen Robert Skov Wolfgang Hoffmann Andreas Peschel Christopher Weidenmaier Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
description	ABSTRACT Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that WTA GlcNAcylation is a pivotal requirement for WTA-dependent attachment of community-acquired methicillin-resistant S. aureus (MRSA) and emerging livestock-associated MRSA to human nasal epithelial cells, even under conditions simulating the nutrient composition and dynamic flow of nasal secretions. Depending on the S. aureus strain, WTA O-GlcNAcylation occurs in either α or β configuration, which have similar capacities to mediate attachment to human nasal epithelial cells, suggesting that many S. aureus strains maintain redundant pathways to ensure appropriate WTA glycosylation. Strikingly, a lack of WTA glycosylation significantly abrogated the ability of MRSA to colonize cotton rat nares in vivo. These results indicate that WTA glycosylation modulates S. aureus nasal colonization and may help to develop new strategies for eradicating S. aureus nasal colonization in the future. IMPORTANCE Nasal colonization by the major human pathogen Staphylococcus aureus is a risk factor for severe endogenous infections and contributes to the spread of this microbe in hospitals and the community. Here, we show that wall teichoic acid (WTA) O-GlcNAcylation is a key factor required for S. aureus nasal colonization. These data provide a mechanistic explanation for the capacity of WTA to modulate S. aureus nasal colonization and may stimulate research activities to establish valuable strategies to eradicate S. aureus nasal colonization in high-risk hospitalized patients and in the general community.
format	article
author	Volker Winstel Petra Kühner Ferdinand Salomon Jesper Larsen Robert Skov Wolfgang Hoffmann Andreas Peschel Christopher Weidenmaier
author_facet	Volker Winstel Petra Kühner Ferdinand Salomon Jesper Larsen Robert Skov Wolfgang Hoffmann Andreas Peschel Christopher Weidenmaier
author_sort	Volker Winstel
title	Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
title_short	Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
title_full	Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
title_fullStr	Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
title_full_unstemmed	Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization
title_sort	wall teichoic acid glycosylation governs <named-content content-type="genus-species">staphylococcus aureus</named-content> nasal colonization
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/5743d590b37e449ebd3f858f2fe094c9
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Wall Teichoic Acid Glycosylation Governs <named-content content-type="genus-species">Staphylococcus aureus</named-content> Nasal Colonization

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