Purpurin suppresses Candida albicans biofilm formation and hyphal development.

A striking and clinically relevant virulence trait of the human fungal pathogen Candida albicans is its ability to grow and switch reversibly among different morphological forms. Inhibition of yeast-to-hypha transition in C. albicans represents a new paradigm for antifungal intervention. We have pre...

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Autores principales:	Paul Wai-Kei Tsang, H M H N Bandara, Wing-Ping Fong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5749182c56224b22ab26ec479f0ebf35
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spelling	oai:doaj.org-article:5749182c56224b22ab26ec479f0ebf352021-11-18T08:06:40ZPurpurin suppresses Candida albicans biofilm formation and hyphal development.1932-620310.1371/journal.pone.0050866https://doaj.org/article/5749182c56224b22ab26ec479f0ebf352012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226409/?tool=EBIhttps://doaj.org/toc/1932-6203A striking and clinically relevant virulence trait of the human fungal pathogen Candida albicans is its ability to grow and switch reversibly among different morphological forms. Inhibition of yeast-to-hypha transition in C. albicans represents a new paradigm for antifungal intervention. We have previously demonstrated the novel antifungal activity of purpurin against Candida fungi. In this study, we extended our investigation by examining the in vitro effect of purpurin on C. albicans morphogenesis and biofilms. The susceptibility of C. albicans biofilms to purpurin was examined quantitatively by 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide reduction assay. Hyphal formation and biofilm ultrastructure were examined qualitatively by scanning electron microscopy (SEM). Quantitative reverse transcription-PCR (qRT-PCR) was used to evaluate the expression of hypha-specific genes and hyphal regulator in purpurin-treated fungal cells. The results showed that, at sub-lethal concentration (3 µg/ml), purpurin blocked the yeast-to-hypha transition under hypha-inducing conditions. Purpurin also inhibited C. albicans biofilm formation and reduced the metabolic activity of mature biofilms in a concentration-dependent manner. SEM images showed that purpurin-treated C. albicans biofilms were scanty and exclusively consisted of aggregates of blastospores. qRT-PCR analyses indicated that purpurin downregulated the expression of hypha-specific genes (ALS3, ECE1, HWP1, HYR1) and the hyphal regulator RAS1. The data strongly suggested that purpurin suppressed C. albicans morphogenesis and caused distorted biofilm formation. By virtue of the ability to block these two virulence traits in C. albicans, purpurin may represent a potential candidate that deserves further investigations in the development of antifungal strategies against this notorious human fungal pathogen in vivo.Paul Wai-Kei TsangH M H N BandaraWing-Ping FongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50866 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paul Wai-Kei Tsang H M H N Bandara Wing-Ping Fong Purpurin suppresses Candida albicans biofilm formation and hyphal development.
description	A striking and clinically relevant virulence trait of the human fungal pathogen Candida albicans is its ability to grow and switch reversibly among different morphological forms. Inhibition of yeast-to-hypha transition in C. albicans represents a new paradigm for antifungal intervention. We have previously demonstrated the novel antifungal activity of purpurin against Candida fungi. In this study, we extended our investigation by examining the in vitro effect of purpurin on C. albicans morphogenesis and biofilms. The susceptibility of C. albicans biofilms to purpurin was examined quantitatively by 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide reduction assay. Hyphal formation and biofilm ultrastructure were examined qualitatively by scanning electron microscopy (SEM). Quantitative reverse transcription-PCR (qRT-PCR) was used to evaluate the expression of hypha-specific genes and hyphal regulator in purpurin-treated fungal cells. The results showed that, at sub-lethal concentration (3 µg/ml), purpurin blocked the yeast-to-hypha transition under hypha-inducing conditions. Purpurin also inhibited C. albicans biofilm formation and reduced the metabolic activity of mature biofilms in a concentration-dependent manner. SEM images showed that purpurin-treated C. albicans biofilms were scanty and exclusively consisted of aggregates of blastospores. qRT-PCR analyses indicated that purpurin downregulated the expression of hypha-specific genes (ALS3, ECE1, HWP1, HYR1) and the hyphal regulator RAS1. The data strongly suggested that purpurin suppressed C. albicans morphogenesis and caused distorted biofilm formation. By virtue of the ability to block these two virulence traits in C. albicans, purpurin may represent a potential candidate that deserves further investigations in the development of antifungal strategies against this notorious human fungal pathogen in vivo.
format	article
author	Paul Wai-Kei Tsang H M H N Bandara Wing-Ping Fong
author_facet	Paul Wai-Kei Tsang H M H N Bandara Wing-Ping Fong
author_sort	Paul Wai-Kei Tsang
title	Purpurin suppresses Candida albicans biofilm formation and hyphal development.
title_short	Purpurin suppresses Candida albicans biofilm formation and hyphal development.
title_full	Purpurin suppresses Candida albicans biofilm formation and hyphal development.
title_fullStr	Purpurin suppresses Candida albicans biofilm formation and hyphal development.
title_full_unstemmed	Purpurin suppresses Candida albicans biofilm formation and hyphal development.
title_sort	purpurin suppresses candida albicans biofilm formation and hyphal development.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5749182c56224b22ab26ec479f0ebf35
work_keys_str_mv	AT paulwaikeitsang purpurinsuppressescandidaalbicansbiofilmformationandhyphaldevelopment AT hmhnbandara purpurinsuppressescandidaalbicansbiofilmformationandhyphaldevelopment AT wingpingfong purpurinsuppressescandidaalbicansbiofilmformationandhyphaldevelopment
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Purpurin suppresses Candida albicans biofilm formation and hyphal development.

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