Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

This study aimed to highlight the relationship between diet, animal performance and mucosal adherent gut microbiota (anterior intestine) in fish fed plant-based diets supplemented with an egg white hydrolysate (EWH) with antioxidant and anti-obesogenic activity in obese rats. The feeding trial with...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fernando Naya-Català, Giulia A. Wiggers, M. Carla Piazzon, Manuel I. López-Martínez, Itziar Estensoro, Josep A. Calduch-Giner, M. Carmen Martínez-Cuesta, Teresa Requena, Ariadna Sitjà-Bobadilla, Marta Miguel, Jaume Pérez-Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioactive peptide egg white hydrolysate gut microbiota bile salts lipid metabolism Sparus aurata Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/574abfd406f8446e91807af14e059c70
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:574abfd406f8446e91807af14e059c70
record_format	dspace
spelling	oai:doaj.org-article:574abfd406f8446e91807af14e059c702021-12-01T07:41:33ZModulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism2296-774510.3389/fmars.2021.698484https://doaj.org/article/574abfd406f8446e91807af14e059c702021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.698484/fullhttps://doaj.org/toc/2296-7745This study aimed to highlight the relationship between diet, animal performance and mucosal adherent gut microbiota (anterior intestine) in fish fed plant-based diets supplemented with an egg white hydrolysate (EWH) with antioxidant and anti-obesogenic activity in obese rats. The feeding trial with juveniles of gilthead sea bream (Sparus aurata) lasted 8 weeks. Fish were fed near to visual satiety with a fish meal (FM)/fish oil (FO) based diet (CTRL) or a plant-based diet with/without EWH supplementation. Specific growth rate decreased gradually from 2.16% in CTRL fish to 1.88% in EWH fish due to a reduced feed intake, and a slight impairment of feed conversion ratio. Plant-based diets feeding triggered a hyperplasic inflammation of the anterior intestine regardless of EWH supplementation. However, EWH ameliorated the goblet cell depletion, and the hepatic and intestinal lipid accumulation induced by FM/FO replacement. Illumina sequencing of gut mucosal microbiota yielded a mean of 136,252 reads per sample assigned to 2,117 OTUs at 97% identity threshold. The bacterial diversity was similar in all groups, but a significantly lower richness was found in EWH fish. At the phylum level, Proteobacteria reached the highest proportion in CTRL and EWH fish, whereas Firmicutes were decreased and Actinobacteria increased with the FM/FO replacement. The proportion of Actinobacteria was restored by dietary EWH supplementation, which also triggered a highest amount of Bacteroidetes and Spirochaetes. At a closer look, a widespread presence of Lactobacillales among groups was found. Otherwise, polysaccharide hydrolases secretors represented by Corynebacterium and Nocardioides were increased by the FM/FO replacement, whereas the mucin-degrading Streptococcus was only raised in fish fed the plant-based diet without EWH. In addition, in EWH fish, a higher abundance of Propionibacterium was related to an increased concentration of intestinal propionate. The antagonism of gut health-promoting propionate with cholesterol could explain the inferred underrepresentation of primary bile acid biosynthesis and steroid degradation pathways in the EWH fish microbiota. Altogether, these results reinforce the central role of gut microbiota in the regulation of host metabolism and lipid metabolism in particular, suggesting a role of the bioactive EWH peptides as an anti-obesity and/or satiety factor in fish.Fernando Naya-CatalàGiulia A. WiggersM. Carla PiazzonManuel I. López-MartínezItziar EstensoroJosep A. Calduch-GinerM. Carmen Martínez-CuestaTeresa RequenaAriadna Sitjà-BobadillaMarta MiguelJaume Pérez-SánchezFrontiers Media S.A.articlebioactive peptideegg white hydrolysategut microbiotabile saltslipid metabolismSparus aurataScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive peptide egg white hydrolysate gut microbiota bile salts lipid metabolism Sparus aurata Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	bioactive peptide egg white hydrolysate gut microbiota bile salts lipid metabolism Sparus aurata Science Q General. Including nature conservation, geographical distribution QH1-199.5 Fernando Naya-Català Giulia A. Wiggers M. Carla Piazzon Manuel I. López-Martínez Itziar Estensoro Josep A. Calduch-Giner M. Carmen Martínez-Cuesta Teresa Requena Ariadna Sitjà-Bobadilla Marta Miguel Jaume Pérez-Sánchez Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
description	This study aimed to highlight the relationship between diet, animal performance and mucosal adherent gut microbiota (anterior intestine) in fish fed plant-based diets supplemented with an egg white hydrolysate (EWH) with antioxidant and anti-obesogenic activity in obese rats. The feeding trial with juveniles of gilthead sea bream (Sparus aurata) lasted 8 weeks. Fish were fed near to visual satiety with a fish meal (FM)/fish oil (FO) based diet (CTRL) or a plant-based diet with/without EWH supplementation. Specific growth rate decreased gradually from 2.16% in CTRL fish to 1.88% in EWH fish due to a reduced feed intake, and a slight impairment of feed conversion ratio. Plant-based diets feeding triggered a hyperplasic inflammation of the anterior intestine regardless of EWH supplementation. However, EWH ameliorated the goblet cell depletion, and the hepatic and intestinal lipid accumulation induced by FM/FO replacement. Illumina sequencing of gut mucosal microbiota yielded a mean of 136,252 reads per sample assigned to 2,117 OTUs at 97% identity threshold. The bacterial diversity was similar in all groups, but a significantly lower richness was found in EWH fish. At the phylum level, Proteobacteria reached the highest proportion in CTRL and EWH fish, whereas Firmicutes were decreased and Actinobacteria increased with the FM/FO replacement. The proportion of Actinobacteria was restored by dietary EWH supplementation, which also triggered a highest amount of Bacteroidetes and Spirochaetes. At a closer look, a widespread presence of Lactobacillales among groups was found. Otherwise, polysaccharide hydrolases secretors represented by Corynebacterium and Nocardioides were increased by the FM/FO replacement, whereas the mucin-degrading Streptococcus was only raised in fish fed the plant-based diet without EWH. In addition, in EWH fish, a higher abundance of Propionibacterium was related to an increased concentration of intestinal propionate. The antagonism of gut health-promoting propionate with cholesterol could explain the inferred underrepresentation of primary bile acid biosynthesis and steroid degradation pathways in the EWH fish microbiota. Altogether, these results reinforce the central role of gut microbiota in the regulation of host metabolism and lipid metabolism in particular, suggesting a role of the bioactive EWH peptides as an anti-obesity and/or satiety factor in fish.
format	article
author	Fernando Naya-Català Giulia A. Wiggers M. Carla Piazzon Manuel I. López-Martínez Itziar Estensoro Josep A. Calduch-Giner M. Carmen Martínez-Cuesta Teresa Requena Ariadna Sitjà-Bobadilla Marta Miguel Jaume Pérez-Sánchez
author_facet	Fernando Naya-Català Giulia A. Wiggers M. Carla Piazzon Manuel I. López-Martínez Itziar Estensoro Josep A. Calduch-Giner M. Carmen Martínez-Cuesta Teresa Requena Ariadna Sitjà-Bobadilla Marta Miguel Jaume Pérez-Sánchez
author_sort	Fernando Naya-Català
title	Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
title_short	Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
title_full	Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
title_fullStr	Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
title_full_unstemmed	Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
title_sort	modulation of gilthead sea bream gut microbiota by a bioactive egg white hydrolysate: interactions between bacteria and host lipid metabolism
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/574abfd406f8446e91807af14e059c70
work_keys_str_mv	AT fernandonayacatala modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT giuliaawiggers modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT mcarlapiazzon modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT manuelilopezmartinez modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT itziarestensoro modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT josepacalduchginer modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT mcarmenmartinezcuesta modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT teresarequena modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT ariadnasitjabobadilla modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT martamiguel modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism AT jaumeperezsanchez modulationofgiltheadseabreamgutmicrobiotabyabioactiveeggwhitehydrolysateinteractionsbetweenbacteriaandhostlipidmetabolism
_version_	1718405412981571584

Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

Ejemplares similares