Single-site glycine-specific labeling of proteins

Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.

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Autores principales:	Landa Purushottam, Srinivasa Rao Adusumalli, Usha Singh, V. B. Unnikrishnan, Dattatraya Gautam Rawale, Mansi Gujrati, Ram Kumar Mishra, Vishal Rai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/574d051de9164761bceab016ed906ff0
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spelling	oai:doaj.org-article:574d051de9164761bceab016ed906ff02021-12-02T14:40:01ZSingle-site glycine-specific labeling of proteins10.1038/s41467-019-10503-72041-1723https://doaj.org/article/574d051de9164761bceab016ed906ff02019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10503-7https://doaj.org/toc/2041-1723Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.Landa PurushottamSrinivasa Rao AdusumalliUsha SinghV. B. UnnikrishnanDattatraya Gautam RawaleMansi GujratiRam Kumar MishraVishal RaiNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Landa Purushottam Srinivasa Rao Adusumalli Usha Singh V. B. Unnikrishnan Dattatraya Gautam Rawale Mansi Gujrati Ram Kumar Mishra Vishal Rai Single-site glycine-specific labeling of proteins
description	Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.
format	article
author	Landa Purushottam Srinivasa Rao Adusumalli Usha Singh V. B. Unnikrishnan Dattatraya Gautam Rawale Mansi Gujrati Ram Kumar Mishra Vishal Rai
author_facet	Landa Purushottam Srinivasa Rao Adusumalli Usha Singh V. B. Unnikrishnan Dattatraya Gautam Rawale Mansi Gujrati Ram Kumar Mishra Vishal Rai
author_sort	Landa Purushottam
title	Single-site glycine-specific labeling of proteins
title_short	Single-site glycine-specific labeling of proteins
title_full	Single-site glycine-specific labeling of proteins
title_fullStr	Single-site glycine-specific labeling of proteins
title_full_unstemmed	Single-site glycine-specific labeling of proteins
title_sort	single-site glycine-specific labeling of proteins
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/574d051de9164761bceab016ed906ff0
work_keys_str_mv	AT landapurushottam singlesiteglycinespecificlabelingofproteins AT srinivasaraoadusumalli singlesiteglycinespecificlabelingofproteins AT ushasingh singlesiteglycinespecificlabelingofproteins AT vbunnikrishnan singlesiteglycinespecificlabelingofproteins AT dattatrayagautamrawale singlesiteglycinespecificlabelingofproteins AT mansigujrati singlesiteglycinespecificlabelingofproteins AT ramkumarmishra singlesiteglycinespecificlabelingofproteins AT vishalrai singlesiteglycinespecificlabelingofproteins
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Single-site glycine-specific labeling of proteins

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