Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ra...

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Autores principales: Diming Zhang, Emily Redington, Yiyang Gong
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/57531aeaa6ef4b899399919ecc2a2943
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Sumario:Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ratiometric calcium sensors in brightness, photobleaching, and signal fidelity metrics.