Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ra...

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Autores principales:	Diming Zhang, Emily Redington, Yiyang Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/57531aeaa6ef4b899399919ecc2a2943
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spelling	oai:doaj.org-article:57531aeaa6ef4b899399919ecc2a29432021-12-02T16:24:51ZRational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins10.1038/s42003-021-02452-z2399-3642https://doaj.org/article/57531aeaa6ef4b899399919ecc2a29432021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02452-zhttps://doaj.org/toc/2399-3642Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ratiometric calcium sensors in brightness, photobleaching, and signal fidelity metrics.Diming ZhangEmily RedingtonYiyang GongNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Diming Zhang Emily Redington Yiyang Gong Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
description	Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ratiometric calcium sensors in brightness, photobleaching, and signal fidelity metrics.
format	article
author	Diming Zhang Emily Redington Yiyang Gong
author_facet	Diming Zhang Emily Redington Yiyang Gong
author_sort	Diming Zhang
title	Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
title_short	Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
title_full	Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
title_fullStr	Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
title_full_unstemmed	Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
title_sort	rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/57531aeaa6ef4b899399919ecc2a2943
work_keys_str_mv	AT dimingzhang rationalengineeringofratiometriccalciumsensorswithbrightgreenandredfluorescentproteins AT emilyredington rationalengineeringofratiometriccalciumsensorswithbrightgreenandredfluorescentproteins AT yiyanggong rationalengineeringofratiometriccalciumsensorswithbrightgreenandredfluorescentproteins
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Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

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