A novel 3D in vitro model of the human gut microbiota

Abstract Clinical trials and animal studies on the gut microbiota are often limited by the difficult access to the gut, restricted possibility of in vivo monitoring, and ethical issues. An easily accessible and monitorable in vitro model of the gut microbiota represents a valid tool for a wider comp...

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Autores principales:	Francesco Biagini, Marco Calvigioni, Anna Lapomarda, Alessandra Vecchione, Chiara Magliaro, Carmelo De Maria, Francesca Montemurro, Francesco Celandroni, Diletta Mazzantini, Monica Mattioli-Belmonte, Emilia Ghelardi, Giovanni Vozzi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57634081c08e498d89d82c508ad377d6
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spelling	oai:doaj.org-article:57634081c08e498d89d82c508ad377d62021-12-02T16:18:04ZA novel 3D in vitro model of the human gut microbiota10.1038/s41598-020-78591-w2045-2322https://doaj.org/article/57634081c08e498d89d82c508ad377d62020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78591-whttps://doaj.org/toc/2045-2322Abstract Clinical trials and animal studies on the gut microbiota are often limited by the difficult access to the gut, restricted possibility of in vivo monitoring, and ethical issues. An easily accessible and monitorable in vitro model of the gut microbiota represents a valid tool for a wider comprehension of the mechanisms by which microbes interact with the host and with each other. Herein, we present a novel and reliable system for culturing the human gut microbiota in vitro. An electrospun gelatin structure was biofabricated as scaffold for microbial growth. The efficiency of this structure in supporting microbial proliferation and biofilm formation was initially assessed for five microbes commonly inhabiting the human gut. The human fecal microbiota was then cultured on the scaffolds and microbial biofilms monitored by confocal laser and scanning electron microscopy and quantified over time. Metagenomic analyses and Real-Time qPCRs were performed to evaluate the stability of the cultured microbiota in terms of qualitative and quantitative composition. Our results reveal the three-dimensionality of the scaffold-adhered microbial consortia that maintain the bacterial biodiversity and richness found in the original sample. These findings demonstrate the validity of the developed electrospun gelatin-based system for in vitro culturing the human gut microbiota.Francesco BiaginiMarco CalvigioniAnna LapomardaAlessandra VecchioneChiara MagliaroCarmelo De MariaFrancesca MontemurroFrancesco CelandroniDiletta MazzantiniMonica Mattioli-BelmonteEmilia GhelardiGiovanni VozziNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesco Biagini Marco Calvigioni Anna Lapomarda Alessandra Vecchione Chiara Magliaro Carmelo De Maria Francesca Montemurro Francesco Celandroni Diletta Mazzantini Monica Mattioli-Belmonte Emilia Ghelardi Giovanni Vozzi A novel 3D in vitro model of the human gut microbiota
description	Abstract Clinical trials and animal studies on the gut microbiota are often limited by the difficult access to the gut, restricted possibility of in vivo monitoring, and ethical issues. An easily accessible and monitorable in vitro model of the gut microbiota represents a valid tool for a wider comprehension of the mechanisms by which microbes interact with the host and with each other. Herein, we present a novel and reliable system for culturing the human gut microbiota in vitro. An electrospun gelatin structure was biofabricated as scaffold for microbial growth. The efficiency of this structure in supporting microbial proliferation and biofilm formation was initially assessed for five microbes commonly inhabiting the human gut. The human fecal microbiota was then cultured on the scaffolds and microbial biofilms monitored by confocal laser and scanning electron microscopy and quantified over time. Metagenomic analyses and Real-Time qPCRs were performed to evaluate the stability of the cultured microbiota in terms of qualitative and quantitative composition. Our results reveal the three-dimensionality of the scaffold-adhered microbial consortia that maintain the bacterial biodiversity and richness found in the original sample. These findings demonstrate the validity of the developed electrospun gelatin-based system for in vitro culturing the human gut microbiota.
format	article
author	Francesco Biagini Marco Calvigioni Anna Lapomarda Alessandra Vecchione Chiara Magliaro Carmelo De Maria Francesca Montemurro Francesco Celandroni Diletta Mazzantini Monica Mattioli-Belmonte Emilia Ghelardi Giovanni Vozzi
author_facet	Francesco Biagini Marco Calvigioni Anna Lapomarda Alessandra Vecchione Chiara Magliaro Carmelo De Maria Francesca Montemurro Francesco Celandroni Diletta Mazzantini Monica Mattioli-Belmonte Emilia Ghelardi Giovanni Vozzi
author_sort	Francesco Biagini
title	A novel 3D in vitro model of the human gut microbiota
title_short	A novel 3D in vitro model of the human gut microbiota
title_full	A novel 3D in vitro model of the human gut microbiota
title_fullStr	A novel 3D in vitro model of the human gut microbiota
title_full_unstemmed	A novel 3D in vitro model of the human gut microbiota
title_sort	novel 3d in vitro model of the human gut microbiota
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/57634081c08e498d89d82c508ad377d6
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A novel 3D in vitro model of the human gut microbiota

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