Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model

Abstract Multibipolar radiofrequency ablation (RFA) is an advanced ablation technique for early stage hepatocellular carcinoma and liver metastases. Vessel cooling in multibipolar RFA has not been systematically investigated. The objective of this study was to evaluate the presence of perivascular v...

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Autores principales:	F. G. M. Poch, C. A. Neizert, B. Geyer, O. Gemeinhardt, S. M. Niehues, J. L. Vahldiek, K. K. Bressem, K. S. Lehmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5765e94f758e4070ba3b3822ccaa7d64
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spelling	oai:doaj.org-article:5765e94f758e4070ba3b3822ccaa7d642021-12-02T15:39:50ZPerivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model10.1038/s41598-021-93406-22045-2322https://doaj.org/article/5765e94f758e4070ba3b3822ccaa7d642021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93406-2https://doaj.org/toc/2045-2322Abstract Multibipolar radiofrequency ablation (RFA) is an advanced ablation technique for early stage hepatocellular carcinoma and liver metastases. Vessel cooling in multibipolar RFA has not been systematically investigated. The objective of this study was to evaluate the presence of perivascular vital cells within the ablation zone after multibipolar RFA. Multibipolar RFA were performed in domestic pigs in vivo. Three internally cooled bipolar RFA applicators were used simultaneously. Three experimental settings were planned: (1) inter-applicator-distance: 15 mm; (2) inter-applicator-distance: 20 mm; (3) inter-applicator-distance: 20 mm with hepatic inflow occlusion (Pringle maneuver). A vitality staining was used to analyze liver cell vitality around all vessels in the ablation center with a diameter > 0.5 mm histologically. 771 vessels were identified. No vital tissue was seen around 423 out of 429 vessels (98.6%) situated within the central white zone. Vital cells could be observed around major hepatic vessels situated adjacent to the ablation center. Vessel diameter (> 3.0 mm; p < 0.05) and low vessel-to-ablation-center distance (< 0.2 mm; p < 0.05) were identified as risk factors for incomplete ablation adjacent to hepatic vessels. The vast majority of vessels, which were localized in the clinically relevant white zone, showed no vital perivascular cells, regardless of vessel diameter and vessel type. However, there was a risk of incomplete ablation around major hepatic vessels situated directly within the ablation center. A Pringle maneuver could avoid incomplete ablations.F. G. M. PochC. A. NeizertB. GeyerO. GemeinhardtS. M. NiehuesJ. L. VahldiekK. K. BressemK. S. LehmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. G. M. Poch C. A. Neizert B. Geyer O. Gemeinhardt S. M. Niehues J. L. Vahldiek K. K. Bressem K. S. Lehmann Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
description	Abstract Multibipolar radiofrequency ablation (RFA) is an advanced ablation technique for early stage hepatocellular carcinoma and liver metastases. Vessel cooling in multibipolar RFA has not been systematically investigated. The objective of this study was to evaluate the presence of perivascular vital cells within the ablation zone after multibipolar RFA. Multibipolar RFA were performed in domestic pigs in vivo. Three internally cooled bipolar RFA applicators were used simultaneously. Three experimental settings were planned: (1) inter-applicator-distance: 15 mm; (2) inter-applicator-distance: 20 mm; (3) inter-applicator-distance: 20 mm with hepatic inflow occlusion (Pringle maneuver). A vitality staining was used to analyze liver cell vitality around all vessels in the ablation center with a diameter > 0.5 mm histologically. 771 vessels were identified. No vital tissue was seen around 423 out of 429 vessels (98.6%) situated within the central white zone. Vital cells could be observed around major hepatic vessels situated adjacent to the ablation center. Vessel diameter (> 3.0 mm; p < 0.05) and low vessel-to-ablation-center distance (< 0.2 mm; p < 0.05) were identified as risk factors for incomplete ablation adjacent to hepatic vessels. The vast majority of vessels, which were localized in the clinically relevant white zone, showed no vital perivascular cells, regardless of vessel diameter and vessel type. However, there was a risk of incomplete ablation around major hepatic vessels situated directly within the ablation center. A Pringle maneuver could avoid incomplete ablations.
format	article
author	F. G. M. Poch C. A. Neizert B. Geyer O. Gemeinhardt S. M. Niehues J. L. Vahldiek K. K. Bressem K. S. Lehmann
author_facet	F. G. M. Poch C. A. Neizert B. Geyer O. Gemeinhardt S. M. Niehues J. L. Vahldiek K. K. Bressem K. S. Lehmann
author_sort	F. G. M. Poch
title	Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
title_short	Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
title_full	Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
title_fullStr	Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
title_full_unstemmed	Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
title_sort	perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5765e94f758e4070ba3b3822ccaa7d64
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Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model

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