Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification

There is a need to further improve the automation of cryo-EM sample preparation to make it more easily accessible for non-specialists, reduce sample waste and increase reproducibility. Here, the authors present VitroJet, a single device, where sub-nl volumes of samples are deposited by pin printing...

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Autores principales:	Raimond B. G. Ravelli, Frank J. T. Nijpels, Rene J. M. Henderikx, Giulia Weissenberger, Sanne Thewessem, Abril Gijsbers, Bart W. A. M. M. Beulen, Carmen López-Iglesias, Peter J. Peters
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/576baa0f76434676a7786827d52416e0
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id	oai:doaj.org-article:576baa0f76434676a7786827d52416e0
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spelling	oai:doaj.org-article:576baa0f76434676a7786827d52416e02021-12-02T15:52:52ZCryo-EM structures from sub-nl volumes using pin-printing and jet vitrification10.1038/s41467-020-16392-52041-1723https://doaj.org/article/576baa0f76434676a7786827d52416e02020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16392-5https://doaj.org/toc/2041-1723There is a need to further improve the automation of cryo-EM sample preparation to make it more easily accessible for non-specialists, reduce sample waste and increase reproducibility. Here, the authors present VitroJet, a single device, where sub-nl volumes of samples are deposited by pin printing thus eliminating the need for sample blotting, which is followed by jet vitrification, and they show that high-resolution structures can be obtained using four standard proteins.Raimond B. G. RavelliFrank J. T. NijpelsRene J. M. HenderikxGiulia WeissenbergerSanne ThewessemAbril GijsbersBart W. A. M. M. BeulenCarmen López-IglesiasPeter J. PetersNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Raimond B. G. Ravelli Frank J. T. Nijpels Rene J. M. Henderikx Giulia Weissenberger Sanne Thewessem Abril Gijsbers Bart W. A. M. M. Beulen Carmen López-Iglesias Peter J. Peters Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
description	There is a need to further improve the automation of cryo-EM sample preparation to make it more easily accessible for non-specialists, reduce sample waste and increase reproducibility. Here, the authors present VitroJet, a single device, where sub-nl volumes of samples are deposited by pin printing thus eliminating the need for sample blotting, which is followed by jet vitrification, and they show that high-resolution structures can be obtained using four standard proteins.
format	article
author	Raimond B. G. Ravelli Frank J. T. Nijpels Rene J. M. Henderikx Giulia Weissenberger Sanne Thewessem Abril Gijsbers Bart W. A. M. M. Beulen Carmen López-Iglesias Peter J. Peters
author_facet	Raimond B. G. Ravelli Frank J. T. Nijpels Rene J. M. Henderikx Giulia Weissenberger Sanne Thewessem Abril Gijsbers Bart W. A. M. M. Beulen Carmen López-Iglesias Peter J. Peters
author_sort	Raimond B. G. Ravelli
title	Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
title_short	Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
title_full	Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
title_fullStr	Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
title_full_unstemmed	Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
title_sort	cryo-em structures from sub-nl volumes using pin-printing and jet vitrification
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/576baa0f76434676a7786827d52416e0
work_keys_str_mv	AT raimondbgravelli cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT frankjtnijpels cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT renejmhenderikx cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT giuliaweissenberger cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT sannethewessem cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT abrilgijsbers cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT bartwammbeulen cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT carmenlopeziglesias cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification AT peterjpeters cryoemstructuresfromsubnlvolumesusingpinprintingandjetvitrification
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Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification

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