The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
The fungus <i>F. pseudograminearum</i> can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regu...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/57768c61a7ef482c953f7d1565184819 |
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| Sumario: | The fungus <i>F. pseudograminearum</i> can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regulatory function of the cysteine-rich repeat receptor-like kinase TaCRK-7A in the defense against <i>F. pseudograminearum</i> in wheat. Antifungal assays showed that the purified TaCRK-7A protein inhibited the growth of <i>F. pseudograminearum</i>. <i>TaCRK-7A</i> transcript abundance was elevated after <i>F. pseudograminearum</i> attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of <i>TaCRK-7A</i> transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. Furthermore, the transcript abundances of <i>TaCRK-7A</i> and its modulated-defense genes were responsive to exogenous jasmonate treatment. Taken together, these results suggest that TaCRK-7A can directly inhibit <i>F. pseudograminearum</i> growth and mediates FCR-resistance by promoting the expression of wheat defense genes in the jasmonate pathway. Thus, <i>TaCRK-7A</i> is a potential gene resource in FCR-resistant wheat breeding program. |
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