The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat

The fungus <i>F. pseudograminearum</i> can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regu...

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Autores principales:	Tianci Wu, Feilong Guo, Gangbiao Xu, Jinfeng Yu, Li Zhang, Xuening Wei, Xiuliang Zhu, Zengyan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antifungal activity receptor-like kinase with DUF26 domain-containing Fusarium crown rot resistance <i>Fusarium pseudograminearum</i> wheat (<i>Triticum aestivum</i>) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/57768c61a7ef482c953f7d1565184819
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spelling	oai:doaj.org-article:57768c61a7ef482c953f7d15651848192021-11-25T16:47:12ZThe Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat10.3390/biology101111222079-7737https://doaj.org/article/57768c61a7ef482c953f7d15651848192021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1122https://doaj.org/toc/2079-7737The fungus <i>F. pseudograminearum</i> can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regulatory function of the cysteine-rich repeat receptor-like kinase TaCRK-7A in the defense against <i>F. pseudograminearum</i> in wheat. Antifungal assays showed that the purified TaCRK-7A protein inhibited the growth of <i>F. pseudograminearum</i>. <i>TaCRK-7A</i> transcript abundance was elevated after <i>F. pseudograminearum</i> attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of <i>TaCRK-7A</i> transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. Furthermore, the transcript abundances of <i>TaCRK-7A</i> and its modulated-defense genes were responsive to exogenous jasmonate treatment. Taken together, these results suggest that TaCRK-7A can directly inhibit <i>F. pseudograminearum</i> growth and mediates FCR-resistance by promoting the expression of wheat defense genes in the jasmonate pathway. Thus, <i>TaCRK-7A</i> is a potential gene resource in FCR-resistant wheat breeding program.Tianci WuFeilong GuoGangbiao XuJinfeng YuLi ZhangXuening WeiXiuliang ZhuZengyan ZhangMDPI AGarticleantifungal activityreceptor-like kinase with DUF26 domain-containingFusarium crown rot resistance<i>Fusarium pseudograminearum</i>wheat (<i>Triticum aestivum</i>)Biology (General)QH301-705.5ENBiology, Vol 10, Iss 1122, p 1122 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antifungal activity receptor-like kinase with DUF26 domain-containing Fusarium crown rot resistance <i>Fusarium pseudograminearum</i> wheat (<i>Triticum aestivum</i>) Biology (General) QH301-705.5
spellingShingle	antifungal activity receptor-like kinase with DUF26 domain-containing Fusarium crown rot resistance <i>Fusarium pseudograminearum</i> wheat (<i>Triticum aestivum</i>) Biology (General) QH301-705.5 Tianci Wu Feilong Guo Gangbiao Xu Jinfeng Yu Li Zhang Xuening Wei Xiuliang Zhu Zengyan Zhang The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
description	The fungus <i>F. pseudograminearum</i> can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regulatory function of the cysteine-rich repeat receptor-like kinase TaCRK-7A in the defense against <i>F. pseudograminearum</i> in wheat. Antifungal assays showed that the purified TaCRK-7A protein inhibited the growth of <i>F. pseudograminearum</i>. <i>TaCRK-7A</i> transcript abundance was elevated after <i>F. pseudograminearum</i> attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of <i>TaCRK-7A</i> transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. Furthermore, the transcript abundances of <i>TaCRK-7A</i> and its modulated-defense genes were responsive to exogenous jasmonate treatment. Taken together, these results suggest that TaCRK-7A can directly inhibit <i>F. pseudograminearum</i> growth and mediates FCR-resistance by promoting the expression of wheat defense genes in the jasmonate pathway. Thus, <i>TaCRK-7A</i> is a potential gene resource in FCR-resistant wheat breeding program.
format	article
author	Tianci Wu Feilong Guo Gangbiao Xu Jinfeng Yu Li Zhang Xuening Wei Xiuliang Zhu Zengyan Zhang
author_facet	Tianci Wu Feilong Guo Gangbiao Xu Jinfeng Yu Li Zhang Xuening Wei Xiuliang Zhu Zengyan Zhang
author_sort	Tianci Wu
title	The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
title_short	The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
title_full	The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
title_fullStr	The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
title_full_unstemmed	The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat
title_sort	receptor-like kinase tacrk-7a inhibits <i>fusarium pseudograminearum</i> growth and mediates resistance to fusarium crown rot in wheat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/57768c61a7ef482c953f7d1565184819
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The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat

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