Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Pierre-Alexis Mouthuy,1,2 Maja Somogyi &Scaron;koc,3 Ana Čipak Ga&scaron;parović,1 Lidija Milković,1 Andrew J Carr,2 Neven Žarković1 1Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Zagreb, Croatia; 2Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and M...

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Autores principales:	Mouthuy PA, Somogyi Škoc M, Čipak Gašparović A, Milković L, Carr AJ, Žarković N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	electrospinning electrospun filaments poly-p-dioxanone PDO curcumin human fibroblasts Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/57793779016f43919f2ff34899c3b2f5
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spelling	oai:doaj.org-article:57793779016f43919f2ff34899c3b2f52021-12-02T05:10:27ZInvestigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications1178-2013https://doaj.org/article/57793779016f43919f2ff34899c3b2f52017-05-01T00:00:00Zhttps://www.dovepress.com/investigating-the-use-of-curcumin-loaded-electrospun-filaments-for-sof-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pierre-Alexis Mouthuy,1,2 Maja Somogyi &Scaron;koc,3 Ana Čipak Ga&scaron;parović,1 Lidija Milković,1 Andrew J Carr,2 Neven Žarković1 1Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Zagreb, Croatia; 2Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Medical Science Division, University of Oxford, Oxford, UK; 3Department of Materials, Fibres and Textile Testing, University of Zagreb, Zagreb, Croatia Abstract: Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 µM, respectively), which are near or above the known toxicity threshold of curcumin (~10 µM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters. Keywords: electrospinning, electrospun filaments, polydioxanone, PDO, curcumin, human fibroblastsMouthuy PASomogyi Škoc MČipak Gašparović AMilković LCarr AJŽarković NDove Medical Pressarticleelectrospinningelectrospun filamentspoly-p-dioxanonePDOcurcuminhuman fibroblastsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3977-3991 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrospinning electrospun filaments poly-p-dioxanone PDO curcumin human fibroblasts Medicine (General) R5-920
spellingShingle	electrospinning electrospun filaments poly-p-dioxanone PDO curcumin human fibroblasts Medicine (General) R5-920 Mouthuy PA Somogyi Škoc M Čipak Gašparović A Milković L Carr AJ Žarković N Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
description	Pierre-Alexis Mouthuy,1,2 Maja Somogyi &Scaron;koc,3 Ana Čipak Ga&scaron;parović,1 Lidija Milković,1 Andrew J Carr,2 Neven Žarković1 1Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Zagreb, Croatia; 2Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Medical Science Division, University of Oxford, Oxford, UK; 3Department of Materials, Fibres and Textile Testing, University of Zagreb, Zagreb, Croatia Abstract: Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 µM, respectively), which are near or above the known toxicity threshold of curcumin (~10 µM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters. Keywords: electrospinning, electrospun filaments, polydioxanone, PDO, curcumin, human fibroblasts
format	article
author	Mouthuy PA Somogyi Škoc M Čipak Gašparović A Milković L Carr AJ Žarković N
author_facet	Mouthuy PA Somogyi Škoc M Čipak Gašparović A Milković L Carr AJ Žarković N
author_sort	Mouthuy PA
title	Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
title_short	Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
title_full	Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
title_fullStr	Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
title_full_unstemmed	Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
title_sort	investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/57793779016f43919f2ff34899c3b2f5
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_version_	1718400530097635328

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

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